scholarly journals Multicentric Evaluation of a Novel Point of Care Electrochemical ELISA Platform for SARS-CoV-2 Specific IgG and IgM Antibody Assay

2021 ◽  
pp. 114275
Author(s):  
Vinay Kumar ◽  
Kanad Ghosh ◽  
Anagha Chandran ◽  
Sachin Panwar ◽  
Ananthram Bhat ◽  
...  
Keyword(s):  
Point Of Care ◽  
Antibody Assay ◽  
Igm Antibody ◽  
Specific Igg

scholarly journals Age significantly influences the sensitivity of SARS-CoV-2 rapid antibody assays

2021 ◽  
Author(s):  
Natalie Irwin ◽  
Lyle Murray ◽  
Benjamin Ozynski ◽  
Guy A Richards ◽  
Graham Paget ◽  
...  
Keyword(s):  
Point Of Care ◽  
Clinical Context ◽  
Antibody Assay ◽  
Patient Factors ◽  
Assay Sensitivity ◽  
Promising Tool ◽  
Finger Prick ◽  
Antibody Assays ◽  
Specific Igg ◽  
Rapid Antibody

ABSTRACTBACKGROUNDPoint of care serological assays are a promising tool in COVID-19 diagnostics but do have limitations. This study evaluated the sensitivity of five rapid antibody assays and explored factors influencing their sensitivity to detect SARS-CoV-2-specific IgG and IgM antibodies.METHODSFinger-prick blood samples from 102 participants, within two to six weeks of PCR-confirmed COVID-19 diagnosis, were tested for IgG and IgM on five rapid serological assays. The assay sensitivities were compared, and patient factors evaluated in order to investigate potential associations with assay sensitivity.RESULTSSensitivity ranged from 36% to 69% for IgG and 13% to 67% for IgM. Age was the only factor significantly influencing the likelihood of a detectable IgG or IgM response. Individuals aged 40 years and older had an increased likelihood of a detectable IgG or IgM antibody response by rapid antibody assay.CONCLUSIONRapid serological assays demonstrate significant variability when used in a real-world clinical context. There may be limitations in their use for COVID-19 diagnosis amongst the young.

scholarly journals Fetal infection resulting from maternal rubella after the first trimester of pregnancy

1980 ◽  
Vol 85 (3) ◽  
pp. 381-391 ◽  
Author(s):  
J. E. Cradock-Watson ◽  
Margaret K. S. Ridehalgh ◽  
Mary J. Anderson ◽  
J. R. Pattison ◽  
H. O. Kangro
Keyword(s):  
First Trimester ◽  
Menstrual Period ◽  
Last Menstrual Period ◽  
Igg Antibody ◽  
True Rate ◽  
Igm Antibody ◽  
Prenatal Infection ◽  
Fetal Infection ◽  
First Trimester Of Pregnancy ◽  
Specific Igg

SUMMARYWe have tried to measure the incidence of prenatal infection in 304 infants whose mothers had had rubella at various times after the first 12 weeks of pregnancy. Two methods of assessment were used: first, serum obtained soon after birth was tested for specific IgM antibody; secondly, serum obtained after the age of eight months was tested for specific IgG. When maternal rubella occurred 12–16 weeks after the last menstrual period specific IgM antibody was detected in 28 out of 50 infants (56%). The proportion fell progressively to 12% after maternal rubella at 24–28 weeks, rose to 19% after rubella at 28–36 weeks and then to 58% when the illness occurred during the last month of pregnancy. In all, IgM antibody was detected in 77 out of 260 infants (29%). The fetus can thus be infected at any time during the second and third trimesters of pregnancy, but the risk varies at different stages.The figures for the prevalence of IgG antibody were greater throughout, because some infants had IgG who had previously lacked specific IgM. After maternal rubella at 12–16 weeks IgG antibody persisted in 22 out of 31 infants (71%). The proportion fell to 28% after rubella at 24–28 weeks and then increased progressively to 94% after rubella during the last month. In all, IgG antibody persisted in 94 out of 190 infants (49%). The true rate of fetal infection probably lies between the rates estimated from the presence of IgM antibody and the subsequent prevalence of IgG.Infants whose mothers had rubella at any time during pregnancy should be examined regularly for possible evidence of damage.

scholarly journals Regional Difference in Seroprevalence of SARS-CoV-2 in Tokyo: Results from the community point-of-care antibody testing

2020 ◽  
Author(s):  
Morihito Takita ◽  
Tomoko Matsumura ◽  
Kana Yamamoto ◽  
Erika Yamashita ◽  
Kazutaka Hosoda ◽  
...  
Keyword(s):  
Regional Difference ◽  
Point Of Care ◽  
Herd Immunity ◽  
Antibody Testing ◽  
Igg Antibody ◽  
Community Based ◽  
Entire Cohort ◽  
Control And Prevention ◽  
Specific Igg ◽  
Polymerase Chain

AbstractThe serosurvey is an alternative way to know the magnitude of the population infected by coronavirus disease 2019 (COVID-19) since the expansion of capacity of the polymerase chain reaction (PCR) to detect the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was delayed. We herein report seroprevalence of COVID-19 accessed in the two community clinics in Tokyo. The point-of-care immunodiagnostic test was implemented to detect the SARS-CoV-2 specific IgG antibody in the peripheral capillary blood. The overall positive percentage of SARS-CoV-2 IgG antibody is 3.83% (95% confidence interval: 2.76-5.16) for the entire cohort (n =1,071). The central Tokyo of 23 special wards exhibited a significantly higher prevalence compared to the other area of Tokyo (p =0.02, 4.68% [95%CI: 3.08-6.79] versus 1.83 [0.68-3.95] in central and suburban Tokyo, respectively). The seroprevalence of the cohort surveyed in this study is low for herd immunity, which suggests the need for robust disease control and prevention. A community-based approach, rather than state or prefectural levels, is of importance to figure out profiles of the SARS-COV-2 outbreak.

Prevalence of Legionella-specific IgG and IgM Antibody in a Dental Clinic Population

1985 ◽  
Vol 64 (12) ◽  
pp. 1382-1385 ◽  
Author(s):  
P.G. Fotos ◽  
H.N. Westfall ◽  
I.S. Snyder ◽  
R.W. Miller ◽  
B.M. Mutchler
Keyword(s):  
Dental Clinic ◽  
Igm Antibody ◽  
Specific Igg ◽  
Clinic Population

scholarly journals P1-S4.30 Point-of-Care HIV Testing with OraQuick Advance HIV-1/2 antibody assay: a systematic review of cost outcomes

2011 ◽  
Vol 87 (Suppl 1) ◽  
pp. A173-A173
Author(s):  
B. Balram ◽  
M. Johri ◽  
D. Ako-Arrey ◽  
G. Lambert ◽  
C. Claessens ◽  
...  
Keyword(s):  
Systematic Review ◽  
Hiv Testing ◽  
Point Of Care ◽  
Antibody Assay ◽  
Cost Outcomes ◽  
Hiv 1

Variation in serum immunoglobulin levels in acute trichinosis

1977 ◽  
Vol 51 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Robert M. Matossian ◽  
Ibrahim Salti ◽  
Edouard Stephan
Keyword(s):  
Trichinella Spiralis ◽  
Serum Immunoglobulin ◽  
Serum Ige ◽  
Clinical Recovery ◽  
Igm Antibody ◽  
Antibody Titres ◽  
Specific Igg ◽  
Early Rise ◽  
Immunoglobulin Levels

ABSTRACTAn early rise in serum IgE levels was observed in five of seven patients with acute primary trichinosis. Parallel increases in the total IgG, IgM and IgA levels, as well as specific IgG and IgM antibody titres to Trichinella spiralis larval antigen, were detected in two. The elevated levels persisted long after the clinical recovery of the patients.

scholarly journals Multicentric Evaluation of a Novel Point of Care Electrochemical ELISA Platform for SARS-CoV-2 Specific IgG and IgM Antibody Assay

2021 ◽  
Author(s):  
Vinay Kumar ◽  
Kanad Ghosh ◽  
Anagha Chandran ◽  
Sachin Panwar ◽  
Ananthram Bhat ◽  
...  
Keyword(s):  
Quantitative Estimation ◽  
Point Of Care ◽  
Clinical Samples ◽  
Antibody Concentration ◽  
Serum Samples ◽  
Vaccination Programs ◽  
Efficient Detection ◽  
Epidemiological Surveys ◽  
Specific Igg ◽  
Electrochemical Redox

New diagnostics technologies for the efficient detection and quantification of SARS-CoV-2 Antibodies is very crucial to manage the COVID-19 pandemic, especially in the context of emerging vaccination paradigms. Herein, we report on a novel point-of-care Electrochemical ELISA platform with disposable screen printed electrodes functionalized with SARS-CoV-2 Spike Glycoprotein S1, to enable fast and accurate quantitative estimation of total antibody concentration (IgG and IgM) in clinical samples. The quantification is performed with a comparison of electrochemical redox current against the current produced by the spiked monoclonal antibodies with known concentration. The assay is validated through multicentric evaluation against 3 different FDA authorized Laboratory standard techniques, using both EDTA whole blood and serum samples. We demonstrate that the proposed assay has excellent sensitivity and specificity, making it a suitable candidate for epidemiological surveys and quantification of antibodies in COVID-19 vaccination programs.

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