Prevalence and genetic characterization of Pseudomonas aeruginosa in drinking water in Guangdong Province of China

ABSTRACT Type a flagellins from two strains of Pseudomonas aeruginosa, strains PAK and JJ692, were found to be glycosylated with unique glycan structures. In both cases, two sites of O-linked glycosylation were identified on each monomer, and these sites were localized to the central, surface-exposed domain of the monomer in the assembled filament. The PAK flagellin was modified with a heterogeneous glycan comprising up to 11 monosaccharide units that were O linked through a rhamnose residue to the protein backbone. The flagellin of JJ692 was less complex and had a single rhamnose substitution at each site. The role of the glycosylation island gene cluster in the production of each of these glycosyl moieties was investigated. These studies revealed that the orfA and orfN genes were required for attachment of the heterologous glycan and the proximal rhamnose residue, respectively.

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Genetic Characterization of Carbapenemase-Producing Enterobacter cloacae Complex and Pseudomonas aeruginosa of Food of Animal Origin from Egypt

Microbial Drug Resistance ◽

10.1089/mdr.2019.0405 ◽

2020 ◽

Cited By ~ 3

Author(s):

Mustafa Sadek ◽

Ahmed M. Soliman ◽

Hirofumi Nariya ◽

Toshi Shimamoto ◽

Tadashi Shimamoto

Keyword(s):

Pseudomonas Aeruginosa ◽

Genetic Characterization ◽

Enterobacter Cloacae ◽

Animal Origin ◽

Food Of Animal Origin ◽

Enterobacter Cloacae Complex

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Importance of flagella and enterotoxins for Aeromonas virulence in a mouse model

Canadian Journal of Microbiology ◽

10.1139/w06-095 ◽

2007 ◽

Vol 53 (2) ◽

pp. 261-269 ◽

Cited By ~ 14

Author(s):

Keya Sen ◽

Dennis Lye

Keyword(s):

Drinking Water ◽

Virulence Factor ◽

Genetic Characterization ◽

Aeromonas Veronii ◽

Aeromonas Caviae ◽

Polymerase Chain ◽

Immunocompromised Mice ◽

Immunocompromised Hosts ◽

Virulence Factor Genes

A genetic characterization of eight virulence factor genes, elastase, lipase, polar flagella (flaA/flaB, flaG), lateral flagella (lafA), and the enterotoxins alt, act, and ast, was performed using polymerase chain reaction with 55 drinking water and nine clinical isolates. When 16 Aeromonas hydrophila strains, seven Aeromonas veronii strains, and seven Aeromonas caviae strains exhibiting different combinations of virulence factor genes were tested in immunocompromised mice by intraperitoneal injection, only those strains that had one or more of the enterotoxins flaA, flaB, and either flaG or lafA showed signs of being virulent. The correlation was seen in 97% (29/30) of the strains, which included strains from drinking water. Thus, Aeromonas water isolates have the potential to be pathogenic in immunocompromised hosts.

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