scholarly journals Enterococcus faecalis and Enterococcus faecium in pasteurized milk: Prevalence, genotyping, and characterization of virulence traits

LWT ◽  
2022 ◽  
Vol 153 ◽  
pp. 112452
Author(s):  
Mina Nasiri ◽  
Shahram Hanifian
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Pasteurized Milk ◽  
Virulence Traits

scholarly journals Molecular characterization of antimicrobial-resistant Enterococcus faecalis and Enterococcus faecium isolated from layer parent stock

2019 ◽  
Vol 98 (11) ◽  
pp. 5892-5899 ◽  
Author(s):  
Yeong Bin Kim ◽  
Kwang Won Seo ◽  
Jong Bo Shim ◽  
Se hyun Son ◽  
Eun Bi Noh ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Parent Stock

Heterologous Expression and Characterization of Tyrosine Decarboxylase from Enterococcus faecalis R612Z1 and Enterococcus faecium R615Z1

2014 ◽  
Vol 77 (4) ◽  
pp. 592-598 ◽  
Author(s):  
FANG LIU ◽  
WENJUAN XU ◽  
LIHUI DU ◽  
DAOYING WANG ◽  
YONGZHI ZHU ◽  
...  
Keyword(s):  
Amino Acid ◽  
Heterologous Expression ◽  
Molecular Mass ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Maximal Activity ◽  
Tyrosine Decarboxylase ◽  
Acid Protein ◽  
Amino Acid Protein

Tyrosine decarboxylase (TDC) is responsible for tyramine production and can catalyze phenylalanine to produce β-phenylethylamine. Enterococcus strains are a group of bacteria predominantly producing tyramine and β-phenylethylamine in water-boiled salted duck. In this study, the heterologous expression and characterization of two TDCs from Enterococcus faecalis R612Z1 (612TDC) and Enterococcus faecium R615Z1 (615TDC) were studied. The recombinant putative proteins of 612TDC and 615TDC were heterologously expressed in Escherichia coli. 612TDC is a 620-amino-acid protein with a molecular mass of 70.0 kDa, whereas 615TDC is a 625-amino-acid protein with a molecular mass of 70.3 kDa. Both 612TDC and 615TDC showed an optimum temperature of 25°C for the tyrosine and phenylalanine substrates. However, 612TDC revealed maximal activity at pH 5.5, whereas 615TDC revealed maximal activity at pH 6.0. Kinetic studies showed that 612TDC and 615TDC exhibited higher specificity for tyrosine than for phenylalanine. The catalysis abilities of both 612TDC and 615TDC for phenylalanine were restrained significantly with the increase in NaCl concentration, but this was not the case for tyrosine. This study revealed that the enzyme properties of the purified recombinant 612TDC and 615TDC were similar, although their amino acid sequences had 84% identity.

scholarly journals Characterization of vancomycin resistance in Enterococcus faecium and Enterococcus faecalis.

1989 ◽  
Vol 33 (7) ◽  
pp. 1121-1124 ◽  
Author(s):  
T I Nicas ◽  
C Y Wu ◽  
J N Hobbs ◽  
D A Preston ◽  
N E Allen
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Vancomycin Resistance

scholarly journals Incidence of Virulence Factors and Antibiotic Resistance among Enterococci Isolated from Food

2001 ◽  
Vol 67 (9) ◽  
pp. 4385-4389 ◽  
Author(s):  
Charles M. A. P. Franz ◽  
Albrecht B. Muscholl-Silberhorn ◽  
Nuha M. K. Yousif ◽  
Marc Vancanneyt ◽  
Jean Swings ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Enterococcus Faecalis ◽  
Virulence Factors ◽  
Enterococcus Faecium ◽  
Antibiotic Susceptibility ◽  
Virulence Determinants ◽  
Virulence Traits

ABSTRACT The incidence of virulence factors among 48 Enterococcus faecium and 47 Enterococcus faecalis strains from foods and their antibiotic susceptibility were investigated. No strain was resistant to all antibiotics, and for some strains, multiple resistances were observed. Of E. faecium strains, 10.4% were positive for one or more virulence determinants, compared to 78.7% of E. faecalis strains. Strains exhibiting virulence traits were not necessarily positive for all traits; thus, the incidence of virulence factors may be considered to be strain specific.

scholarly journals Detection of Oxazolidinone Resistance Genes and Characterization of Genetic Environments in Enterococci of Swine Origin, Italy

2020 ◽  
Vol 8 (12) ◽  
pp. 2021
Author(s):  
Simona Fioriti ◽  
Gianluca Morroni ◽  
Sonia Nina Coccitto ◽  
Andrea Brenciani ◽  
Alberto Antonelli ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Enterococcus Faecalis ◽  
Resistance Genes ◽  
Enterococcus Faecium ◽  
Central Italy ◽  
Great Variability ◽  
Linezolid Resistance ◽  
Pig Farms ◽  
Clonality Analysis

One hundred forty-five florfenicol-resistant enterococci, isolated from swine fecal samples collected from 76 pig farms, were investigated for the presence of optrA, cfr, and poxtA genes by PCR. Thirty florfenicol-resistant Enterococcus isolates had at least one linezolid resistance gene. optrA was found to be the most widespread linezolid resistance gene (23/30), while cfr and poxtA were detected in 6/30 and 7/30 enterococcal isolates, respectively. WGS analysis also showed the presence of the cfr(D) gene in Enterococcus faecalis (n = 2 isolates) and in Enterococcus avium (n = 1 isolate). The linezolid resistance genes hybridized both on chromosome and plasmids ranging from ~25 to ~240 kb. Twelve isolates were able to transfer linezolid resistance genes to enterococci recipient. WGS analysis displayed a great variability of optrA genetic contexts identical or related to transposons (Tn6628 and Tn6674), plasmids (pE035 and pWo27-9), and chromosomal regions. cfr environments showed identities with Tn6644-like transposon and a region from p12-2300 plasmid; cfr(D) genetic contexts were related to the corresponding region of the plasmid 4 of Enterococcus faecium E8014; poxtA was always found on Tn6657. Circular forms were obtained only for optrA- and poxtA-carrying genetic contexts. Clonality analysis revealed the presence of E. faecalis (ST16, ST27, ST476, and ST585) and E. faecium (ST21) clones previously isolated from humans. These results demonstrate a dissemination of linezolid resistance genes in enterococci of swine origin in Central Italy and confirm the spread of linezolid resistance in animal settings.

Characterization of Enterococcus faecalis and Enterococcus faecium from wild flowers

2011 ◽  
Vol 101 (4) ◽  
pp. 701-711 ◽  
Author(s):  
Antonio Sánchez Valenzuela ◽  
Nabil Benomar ◽  
Hikmate Abriouel ◽  
Rubén Pérez Pulido ◽  
Magdalena Martínez Cañamero ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium

scholarly journals Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France)

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
M. Biendo ◽  
C. Adjidé ◽  
S. Castelain ◽  
M. Belmekki ◽  
F. Rousseau ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecalis ◽  
Genetic Relationship ◽  
Enterococcus Faecium ◽  
Gel Electrophoresis ◽  
Multilocus Sequence Typing ◽  
Pulsed Field Gel Electrophoresis ◽  
Pcr Products ◽  
Sequence Types

We studied 138 glycopeptide-resistant enterococci (GRE) strains, consisting of 131 glycopeptide-resistantEnterococcus faecium(GREfm) and 7 glycopeptide-resistantEnterococcus faecalis(GREfs). The GREfm strains were resistant to penicillin, ampicillin, vancomycin, and teicoplanin, while the GREfs strains were only resistant to vancomycin and teicoplanin. Thevan Agene was the only glycopeptide determinant present in all GRE isolates investigated. Genes coding for Hyl and Hyl+ Esp were detected in 39 (29.8%) and 92 (70.2%) of the 131 GREfm isolates, respectively. Three of the 7 GREfs were positive forgelE+asa 1genes, 3 forgel Egene, and 1 forasa 1gene. The genetic relationship between the 138 GRE was analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). GREfm isolates were clustered in a single genogroup (pulsotype A), and GREfs were clustered in six genogroups (pulsotypes B-G). Among the isolates investigated by MLST, only 18 PCR products were sequenced (12E. faeciumand 6E. faecalis), and 9 sequence types (STs) were identified.

scholarly journals Characterization of the Ends and Target Site of a Novel Tetracycline Resistance-Encoding Conjugative Transposon from Enterococcus faecium 664.1H1

2006 ◽  
Vol 188 (12) ◽  
pp. 4356-4361 ◽  
Author(s):  
Adam P. Roberts ◽  
Ian J. Davis ◽  
Lorna Seville ◽  
Aurelie Villedieu ◽  
Peter Mullany
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Tetracycline Resistance ◽  
Direct Repeat ◽  
Target Site ◽  
Specific Site ◽  
Pathogenicity Islands ◽  
Conjugative Transposon ◽  
Antibiotic Resistant

ABSTRACT Enterococcus faecium 664.1H1 is multiply antibiotic resistant and mercury resistant. In this study, the genetic support for the tetracycline resistance of E. faecium 664.1H1 was characterized. The tet(S) gene is responsible for tetracycline resistance, and this gene is located on the chromosome of E. faecium 664.1H1, on a novel conjugative transposon. The element is transferable to Enterococcus faecalis, where it integrates into a specific site. The element was designated EfcTn1. The integrase of EfcTn1 is related to the integrase proteins found on staphylococcal pathogenicity islands. We show that the transposon is flanked by an 18-bp direct repeat, a copy of which is also present at the target site and at the joint of a circular form, and we propose a mechanism of insertion and excision.

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