Qualitative detection of avian influenza A (H5N1) viruses: A comparative evaluation of four real-time nucleic acid amplification methods

2008 ◽  
Vol 22 (5-6) ◽  
pp. 287-293 ◽  
Author(s):  
Wasun Chantratita ◽  
Chonlaphat Sukasem ◽  
Supaporn Kaewpongsri ◽  
Chutatip Srichunrusami ◽  
Wantanit Pairoj ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Avian Influenza ◽  
Real Time ◽  
Comparative Evaluation ◽  
Influenza A ◽  
Nucleic Acid Amplification ◽  
Qualitative Detection

Evaluation of a rapid isothermal nucleic acid amplification kit, Alere™ i Influenza A&B, for the detection of avian influenza viruses

2019 ◽  
Vol 265 ◽  
pp. 121-125
Author(s):  
Enkhbold Bazarragchaa ◽  
Masatoshi Okamatsu ◽  
Ankhanbaatar Ulaankhuu ◽  
Augustin Tshibwabwa Twabela ◽  
Keita Matsuno ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Avian Influenza ◽  
Influenza A ◽  
Influenza Viruses ◽  
Nucleic Acid Amplification ◽  
Avian Influenza Viruses ◽  
Isothermal Nucleic Acid Amplification

scholarly journals Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses

2021 ◽  
pp. 104063872199481
Author(s):  
Yixin Xiao ◽  
Fan Yang ◽  
Fumin Liu ◽  
Hangping Yao ◽  
Nanping Wu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Influenza A ◽  
Minor Groove Binder ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Pcr Assay ◽  
Infectious Dose ◽  
The Matrix ◽  
Taqman Minor Groove Binder

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50)/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.

scholarly journals Rapid and Highly Sensitive Pathotyping of Avian Influenza A H5N1 Virus by Using Real-Time Reverse Transcription-PCR

2006 ◽  
Vol 45 (2) ◽  
pp. 600-603 ◽  
Author(s):  
B. Hoffmann ◽  
T. Harder ◽  
E. Starick ◽  
K. Depner ◽  
O. Werner ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Real Time ◽  
Reverse Transcription ◽  
Influenza A ◽  
H5n1 Virus ◽  
Reverse Transcription Pcr ◽  
Highly Sensitive

scholarly journals Limited-Resource Preparable Chitosan Magnetic Particles for Extracting Amplification-Ready Zeptomole-Range Nucleic Acid from Complex Biofluid

2021 ◽  
Author(s):  
Sayantan Tripathy ◽  
Arunansu Talukdar ◽  
Goutam Pramanik ◽  
P. V. Rajesh ◽  
Souradyuti Ghosh
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Magnetic Particles ◽  
Limited Resource ◽  
Nucleic Acid Amplification ◽  
Acid Extraction ◽  
Analytical Sensitivity ◽  
Nucleic Acid Extraction ◽  
Spin Column

<b>Layman Summary: </b>Nucleic acid extraction is a key prerequisite for any nucleic acid amplification test (NAAT) or isothermal NAAT (iNAAT) based molecular diagnosis assays.<b> </b>Existing methods utilizes spin column system for nucleic acid extraction which are unsuitable for limited resource settings. Our work explores two methods for chitosan coated magnetic particle preparation that can be executed within 6 h from commonly available chemicals with nothing but a magnetic stirrer and water bath and doable by a minimally trained person. We will also investigated the compatibility of the extracted nucleic acid with downstream NAATs such as real time LAMP, colorimetric LAMP, and real time PCR. In the process, we established the analytical sensitivity of the overall method.<div><br><div><b>Characterization methods</b>: SEM, XRD, EDX, FT-IR</div><div><br></div><div><b>Bioanalytical methods:</b> Real time LAMP, Colorimetric LAMP, Real time PCR</div></div>

Detection of avian influenza A/H7N9/2013 virus by real-time reverse transcription-polymerase chain reaction

2014 ◽  
Vol 206 ◽  
pp. 140-143 ◽  
Author(s):  
Xiaoping Kang ◽  
Weili Wu ◽  
Chuntao Zhang ◽  
Licheng Liu ◽  
Huahua Feng ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Avian Influenza ◽  
Real Time ◽  
Reverse Transcription ◽  
Influenza A ◽  
Chain Reaction ◽  
Polymerase Chain
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