New perspectives for population genetics of Chagas’disease vectors in the Northeastern Brazil: Isolation of polymorphic microsatellite markers in Triatoma brasiliensis

2009 ◽  
Vol 9 (4) ◽  
pp. 633-637 ◽  
Author(s):  
M. Harry ◽  
L. Dupont ◽  
M. Quartier ◽  
L. Diotaiuti ◽  
A. Walter ◽  
...  
Keyword(s):  
Population Genetics ◽  
Microsatellite Markers ◽  
Northeastern Brazil ◽  
Polymorphic Microsatellite

Isolation and characterisation via 454 sequencing of microsatellites from the tawny frogmouth, Podargus strigoides (Class Aves, Family Podargidae)

2012 ◽  
Vol 60 (2) ◽  
pp. 133
Author(s):  
Fiona E. Hogan ◽  
Marian Weaving ◽  
Gregory R. Johnston
Keyword(s):  
Population Genetics ◽  
Gene Flow ◽  
Microsatellite Markers ◽  
Reproductive Ecology ◽  
454 Sequencing ◽  
Urban Environments ◽  
Shotgun Sequencing ◽  
Target Species ◽  
Single Population ◽  
Polymorphic Microsatellite

We isolated 24 novel polymorphic microsatellite markers from the tawny frogmouth, a nocturnal bird endemic to Australia, which has successfully adapted to urban environments. Initially, 454 shotgun sequencing was used to identify 733 loci with primers designed. Of these, we trialled 30 in the target species of which all amplified a product of expected size. Subsequently, all 30 of these loci were screened for variation in 25 individuals, from a single population in Melbourne, Victoria, Australia. Twenty-eight loci were polymorphic with observed heterozygosity ranging from 0.03 to 0.96 (mean 0.58) and the number of alleles per locus ranged from 2 to 18 (average of 6.5); we confirmed that 24 loci conformed to Hardy–Weinberg expectations. The 24 loci identified here will be sufficient to unequivocally identify individuals and will be useful in understanding the reproductive ecology, population genetics and the gene flow amongst localities in urban environments where this bird thrives.

Novel polymorphic microsatellite markers for population genetics of the endangered Caribbean star coral, Montastraea faveolata

2012 ◽  
Vol 43 (2) ◽  
pp. 167-172 ◽  
Author(s):  
Sarah W. Davies ◽  
Muneeb Rahman ◽  
Eli Meyer ◽  
Elizabeth A. Green ◽  
Emmanual Buschiazzo ◽  
...  
Keyword(s):  
Population Genetics ◽  
Microsatellite Markers ◽  
Montastraea Faveolata ◽  
Polymorphic Microsatellite

Isolation and Characterisation of Novel Polymorphic Microsatellite Markers for the Wedge-Billed Woodcreeper Glyphorynchus Spirurus

2017 ◽  
Vol 10 (1) ◽  
pp. 24-26 ◽  
Author(s):  
Jasmin Unrein ◽  
Juliana Menger ◽  
Anne Weigert ◽  
Klaus Henle ◽  
Martin Schlegel
Keyword(s):  
Population Genetics ◽  
Linkage Disequilibrium ◽  
Microsatellite Markers ◽  
Population Genetic ◽  
Ecological Studies ◽  
Central Amazonia ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite ◽  
Generation Sequencing ◽  
Future Population

In order to study the population genetic structure of the Amazonian understorey bird Glyphorynchus spirurus, we have isolated eight polymorphic microsatellite loci using next-generation sequencing. All loci were screened using 40 samples from central Amazonia. The number of alleles varied from five to 22. The observed heterozygosities ranged from 0.475 to 0.875. No linkage disequilibrium was found among the loci. None of the loci showed significant deviation from Hardy–Weinberg equilibrium. These novel microsatellite markers will be a useful tool for future population genetics and ecological studies of the Wedge-billed Woodcreeper.

Development of polymorphic microsatellite markers for the Tertiary relict tree species Taiwania cryptomerioides (Cupressaceae) in East Asia

2021 ◽  
Vol 48 (3) ◽  
pp. 3031-3036
Author(s):  
Mengyun Qin ◽  
Ningning Zhang ◽  
Shixin Zhu ◽  
Caipeng Yue ◽  
Jinyong Huang ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
East Asia ◽  
Tree Species ◽  
Polymorphic Microsatellite ◽  
Taiwania Cryptomerioides

scholarly journals Insights in genetic diversity of German and Italian grape berry moth (Eupoecilia ambiguella) populations using novel microsatellite markers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Annette Reineke ◽  
Alberto Pozzebon ◽  
Olivia Herczynski ◽  
Carlo Duso
Keyword(s):  
Population Genetics ◽  
Microsatellite Markers ◽  
Isolation By Distance ◽  
Resistance Management ◽  
Grape Berry ◽  
Grape Berries ◽  
Eupoecilia Ambiguella ◽  
Significant Damage ◽  
Significant Divergence ◽  
Lepidopteran Pest

AbstractThe grape berry moth Eupoecilia ambiguella (Lepidoptera: Tortricidae) is causing significant damage to grape berries, however, little is known on population genetics of this lepidopteran pest insect, hindered so far by the lack of suitable molecular markers. Here we report on the development of ten microsatellite markers of which six were used to characterise 21 E. ambiguella populations obtained from two viticultural regions in Germany and Italy. Moths were sampled during two subsequent generations (flights) in the same vineyard as well as in vineyards surrounded by different landscape types. German and Italian populations were genetically differentiated and a significant isolation by distance was evident. No significant divergence was observed among the populations from first or second flight moths, however, inbreeding was higher in first than in second flight populations. Moreover, inbreeding was influenced by habitat composition and complexity of landscape around vineyards, being positively associated with the percentage of area covered by grapevine. Population genetics of E. ambiguella could thus be affected by the presence of alternative host plants in viticultural landscapes, which is important in the light of both insecticide resistance management and sustainable pest management.

Genetic characterization of novel polymorphic microsatellite markers for Epilobium nankotaizanense (Onagraceae), an endemic and threatened herb in Taiwan

2021 ◽  
pp. 1-4
Author(s):  
Yu-Wei Tseng ◽  
Chi-Chun Huang ◽  
Chih-Chiang Wang ◽  
Chiuan-Yu Li ◽  
Kuo-Hsiang Hung
Keyword(s):  
Microsatellite Markers ◽  
Natural Populations ◽  
Conservation Strategy ◽  
Sequencing Data ◽  
Germplasm Collections ◽  
The Family ◽  
Conservation Genetic ◽  
Population Sizes ◽  
Polymorphic Microsatellite

Abstract Epilobium belongs to the family Onagraceae, which consists of approximately 200 species distributed worldwide, and some species have been used as medicinal plants. Epilobium nankotaizanense is an endemic and endangered herb that grows in the high mountains in Taiwan at an elevation of more than 3300 m. Alpine herbs are severely threatened by climate change, which leads to a reduction in their habitats and population sizes. However, only a few studies have addressed genetic diversity and population genetics. In the present study, we developed a new set of microsatellite markers for E. nankotaizanense using high-throughput genome sequencing data. Twenty polymorphic microsatellite markers were developed and tested on 30 individuals collected from three natural populations. These loci were successfully amplified, and polymorphisms were observed in E. nankotaizanense. The number of alleles per locus (A) ranged from 2.000 to 3.000, and the observed (Ho) and expected (He) heterozygosities ranged from 0.000 to 0.929 and from 0.034 to 0.631, respectively. The developed polymorphic microsatellite markers will be useful in future conservation genetic studies of E. nankotaizanense as well as for developing an effective conservation strategy for this species and facilitating germplasm collections and sustainable utilization of other Epilobium species.

Characterization of nine polymorphic microsatellite markers in sprat (Sprattus sprattusL.)

2008 ◽  
Vol 8 (4) ◽  
pp. 861-863 ◽  
Author(s):  
T. DAILIANIS ◽  
M. LIMBORG ◽  
R. HANEL ◽  
D. BEKKEVOLD ◽  
J. LAGNEL ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Polymorphic Microsatellite
Sign in / Sign up

Export Citation Format

Share Document