DNA sequence polymorphisms of the pfmdr1 gene and association of mutations with the pfcrt gene in Indian Plasmodium falciparum isolates

2014 ◽  
Vol 26 ◽  
pp. 213-222 ◽  
Author(s):  
Kshipra Chauhan ◽  
Veena Pande ◽  
Aparup Das
Keyword(s):  
Plasmodium Falciparum ◽  
Dna Sequence ◽  
Pfmdr1 Gene ◽  
Pfcrt Gene ◽  
Sequence Polymorphisms

scholarly journals Efficacy of Artemisinin Base Combination Therapy and Genetic Diversity of Plasmodium falciparum from Uncomplicated Ma-laria Falciparum Patient in District of Pesawaran, Province of Lampung, Indonesia

2019 ◽  
Author(s):  
Jhons Fatriyadi SUWANDI ◽  
Widya ASMARA ◽  
Hari KUSNANTO ◽  
Din SYAFRUDDIN ◽  
Supargiyono SUPARGIYONO
Keyword(s):  
Plasmodium Falciparum ◽  
Genetic Variation ◽  
Falciparum Malaria ◽  
Health Centre ◽  
Sequencing Data ◽  
Single Nucleotide ◽  
Pfmdr1 Gene ◽  
Pcr Product ◽  
Nucleotide Mutation ◽  
Reverse Primer

Background: Malaria is an infectious disease caused by Plasmodium sp., that still prevalence in some part of Indonesia. District of Pesawaran is one of malaria endemic area in the Province of Lampung. The purpose of this study was to evaluate the efficacy of the ACT treatment in the District of Pesawaran Province of Lampung, Indonesia from Dec 2012 to Jul 2013 and the genetic variation of the Plasmodium falciparum also studied. Methods: This study was observational analytic study of falciparum malaria patients treated with ACT and primaquine (DHP-PQ and AAQ-PQ) at Hanura Primary Health Centre (Puskesmas). DNA isolation was done with QIAmp DNA Mini Kit. Amplification of PfMDR1, MSP1, and MSP2 genes was done with appropriate forward and reverse primer and procedures optimized first. PCR Product of PfMDR1 gene was prepared for sequencing. Data analysis was done with MEGA 6 software. Results: The results of this research are DHP-PQ effectiveness was still wellness among falciparum malaria patients in District of Pesawaran, Province of Lampung, Indonesia. There is Single-nucleotide mutation of N86Y of PfMDR1 gene. The dominant alleles found are MAD20 and 3D7 alleles with Multiplicity of Infection (MOI) are low. Conclusion: Therapy of DHP-PQ as an antimalarial falciparum in Pesawaran District, Lampung, Indonesia is still good. The genetic variation found was the SNP on the N86Y PfMDR1 gene, with dominant allele MAD20 and 3D7.

DNA sequence artifacts and the estimation of time to the most recent common ancestor (TMRCA) of Plasmodium falciparum

2003 ◽  
Vol 130 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Alyssa E. Barry ◽  
Aleksandra Leliwa ◽  
Mehee Choi ◽  
Kaare M. Nielsen ◽  
Daniel L. Hartl ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Dna Sequence ◽  
Common Ancestor ◽  
Recent Common Ancestor ◽  
Most Recent Common Ancestor ◽  
Estimation Of Time

scholarly journals Mefloquine resistance in Plasmodium falciparum and increased pfmdr1 gene copy number

The Lancet ◽  
2004 ◽  
Vol 364 (9432) ◽  
pp. 438-447 ◽  
Author(s):  
Ric N Price ◽  
Anne-Catrin Uhlemann ◽  
Alan Brockman ◽  
Rose McGready ◽  
Elizabeth Ashley ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Pfmdr1 Gene

scholarly journals Increasedpfmdr1Copy Number and Sequence Polymorphisms in Plasmodium falciparum Isolates from Sudanese Malaria Patients Treated with Artemether-Lumefantrine

2011 ◽  
Vol 55 (11) ◽  
pp. 5408-5411 ◽  
Author(s):  
Nahla B. Gadalla ◽  
Ishag Adam ◽  
Salah-Eldin Elzaki ◽  
Sahar Bashir ◽  
Izdihar Mukhtar ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Molecular Markers ◽  
Dna Sequencing ◽  
Gene Amplification ◽  
Recurrent Infection ◽  
Efficacy Study ◽  
Content Type ◽  
Sequence Polymorphisms ◽  
Eastern Sudan

ABSTRACTMolecular markers for surveillance ofPlasmodium falciparumresistance to current antimalarials are sorely needed. A 28-day efficacy study of artemether-lumefantrine in eastern Sudan identified 5 treatment failures among 100 evaluable patients; 9 further individuals were parasite positive by PCR during follow-up. Polymorphisms inpfatpase6andpfmdr1were evaluated by DNA sequencing. One individual carried parasites with a novelpfmdr1polymorphism (F1044L).pfmdr1gene amplification in parasites prior to treatment occurred in three individuals who had recurrent infection during follow-up.

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