Genetic diversity and phylogeographic analysis of human herpesvirus type 8 (HHV-8) in two distant regions of Argentina: Association with the genetic ancestry of the population

Background: The genetic diversity of persistent infectious agents, such as HHV-8, correlates closely with the migration of modern humans out of East Africa which makes them useful to trace human migrations. However, there is scarce data about the evolutionary history of HHV-8 particularly in multiethnic Latin American populations. Objectives: The aim of this study was to characterize the genetic diversity and the phylogeography of HHV-8 in two distant geographic regions of Argentina and to establish potential associations with the genetic ancestry of the population. Study design: A total of 605 HIV-1 infected subjects, Kaposi Sarcoma (KS) patients and blood donors were recruited in the metropolitan (MET) and north-western regions of Argentina (NWA). After HHV-8 DNA detection, ORF-26 and ORF-K1 were analyzed for subtype assignment. Uniparental and biparental ancestry markers were evaluated in samples in which subtypes could be assigned. Phylogeographic analysis was performed in the ORF-K1 sequences from this study combined with 388 GenBank sequences. Results: HHV-8 was detected in 24.8% of samples. ORF-K1 phylogenetic analyses showed that subtypes A (A1-A5), B1, C (C1-C3) and F were present in 46.9%, 6.25%, 43.75% and 3.1% of cases, respectively. Analyses of ORF-26 fragment revealed that 81.95% of strains were subtypes A/C followed by J, B2, R, and K. Among KS patients, subtype A/C was more commonly detected in MET whereas subtype J was the most frequent in NWA. Subtypes A/C was significantly associated with Native American maternal haplogroups (p=0.004), whereas subtype J was related to non-Native American haplogroups (p<0.0001). Sub-Saharan Africa, Europe and Latin America were the most probable locations from where HHV-8 was introduced to Argentina. Conclusions: These results give evidence of the geographic circulation of HHV-8 in Argentina, provide new insights about its relationship with ancient and modern human migrations and identify the possible origins of this virus in Argentina.

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Triology of Human Herpesvirus Type-8: A Rare and Fatal Case Report of Primary Effusion Lymphoma, Kaposi Sarcoma and Kaposi Sarcoma-associated Herpesvirus Inflammatory Cytokine Syndrome (KICS)

Current Problems in Cancer: Case Reports ◽

10.1016/j.cpccr.2021.100084 ◽

2021 ◽

pp. 100084

Author(s):

Shuai Wang ◽

Lewis Steinberg ◽

Yan Zhang ◽

Carol Harris ◽

Abhishek Kumar

Keyword(s):

Case Report ◽

Inflammatory Cytokine ◽

Primary Effusion Lymphoma ◽

Fatal Case ◽

Human Herpesvirus ◽

Kaposi Sarcoma ◽

Herpesvirus Type ◽

Human Herpesvirus Type

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Early Human Herpesvirus Type 6 Reactivation in Umbilical Cord Blood Allogeneic Stem Cell Transplantation

Biology of Blood and Marrow Transplantation ◽

10.1016/j.bbmt.2013.12.369 ◽

2014 ◽

Vol 20 (2) ◽

pp. S218-S219

Author(s):

Frank Cirrone ◽

Cindy Ippoliti ◽

Koen van Besien

Keyword(s):

Stem Cell ◽

Stem Cell Transplantation ◽

Cord Blood ◽

Umbilical Cord ◽

Allogeneic Stem Cell Transplantation ◽

Umbilical Cord Blood ◽

Human Herpesvirus ◽

Herpesvirus Type ◽

Human Herpesvirus Type ◽

Early Human

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Interaction of Human Immunodeficiency Virus Type 1 and Human Herpesvirus Type 8 Infections on the Incidence of Kaposi's Sarcoma

The Journal of Infectious Diseases ◽

10.1086/315503 ◽

2000 ◽

Vol 181 (6) ◽

pp. 1940-1949 ◽

Cited By ~ 78

Author(s):

L. P. Jacobson ◽

F. J. Jenkins ◽

G. Springer ◽

A. Muñoz ◽

K. V. Shah ◽

...

Keyword(s):

Human Immunodeficiency Virus ◽

Human Immunodeficiency Virus Type ◽

Virus Type ◽

Kaposi's Sarcoma ◽

Human Herpesvirus ◽

Kaposi’S Sarcoma ◽

Herpesvirus Type ◽

Immunodeficiency Virus ◽

Human Herpesvirus Type

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Increased prevalence of human herpesvirus type 8 (HHV-8) genome among blood donors from North-Western Argentina

Journal of Medical Virology ◽

10.1002/jmv.24656 ◽

2016 ◽

Vol 89 (3) ◽

pp. 518-527 ◽

Cited By ~ 3

Author(s):

María Laura Hulaniuk ◽

Oscar Torres ◽

Sonia Bartoli ◽

Lisandro Fortuny ◽

Leandro Burgos Pratx ◽

...

Keyword(s):

Blood Donors ◽

Human Herpesvirus ◽

Herpesvirus Type ◽

Human Herpesvirus Type ◽

North Western

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Immunohistochemical detection of the latent nuclear antigen-1 of the human herpesvirus type 8 to differentiate cutaneous epidemic Kaposi sarcoma and its histological simulators

Anais Brasileiros de Dermatologia ◽

10.1590/s0365-05962013000200010 ◽

2013 ◽

Vol 88 (2) ◽

pp. 243-246 ◽

Cited By ~ 6

Author(s):

Patricia Fonseca Pereira ◽

Tullia Cuzzi ◽

Maria Clara Gutierrez Galhardo

Keyword(s):

Kaposi's Sarcoma ◽

Human Herpesvirus ◽

Kaposi Sarcoma ◽

Kaposi’S Sarcoma ◽

Nuclear Antigen ◽

Immunohistochemical Detection ◽

Herpesvirus Type ◽

Human Herpesvirus Type ◽

Skin Biopsies ◽

Latent Nuclear Antigen

Kaposi's sarcoma is the most common neoplasia diagnosed in AIDS patients and the expression of the human herpesvirus-8 (HHV-8) latent nuclear antigen-1 has been useful for its histological diagnosis. The aim of this study is to confirm that immunohistochemistry is a valuable tool for differentiating KS from its simulators in skin biopsies of HIV patients. Immunohistochemical and histological analyses were performed in 49 Kaposi's sarcoma skin biopsies and 60 of its histological simulators. Positivity was present in the 49 Kaposi's sarcoma skin biopsies and no staining was observed in the 60 simulators analyzed, resulting in sensibility and specificity of 100%. HHV-8 immunohistochemical detection is an effective tool for diagnosing Kaposi's sarcoma, especially in early lesions in which neoplastic features are not evident. It also contributes to its histological differential diagnosis.

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Clinical Aspects of Bacterial Corneal Ulcer Prolonged Course, the Role of Herpes Viruses in Its Pathogenesis, Treatment

Ophthalmology in Russia ◽

10.18008/1816-5095-2019-1s-40-44 ◽

2019 ◽

Vol 16 (1S) ◽

pp. 40-44

Author(s):

V. V. Neroev ◽

L. A. Katargina ◽

L. A. Kovaleva ◽

G. I. Krichevskaya ◽

N. V. Balatskaya

Keyword(s):

Corneal Ulcer ◽

Human Herpesvirus ◽

Clinical Aspects ◽

Herpesvirus Type ◽

Corneal Ulcers ◽

Barr Virus ◽

Human Herpesvirus Type ◽

Epstein Barr ◽

Simplex Virus

Purpose: to study the role of human herpesviruses (HHV) in the pathogenesis of prolonged bacterial corneal ulcers. Patients and methods. 117 patients with bacterial corneal ulcer were examined. Two groups were identified: a favorable course-with duration of bacterial corneal ulcer epithelialization for 17 days (62 people) and a prolonged course with a persistent ulcer more than 17 days (55 people). Blood samples (n = 117) and scrapes from corneal ulcer (n = 117) were investigated in polymerase chain reaction (PCR) for the presence of deoxyribonucleic acid (DNA) of Herpes simplex virus (HSV1, 2), Epstein-Barr virus (EBV), Human herpesvirus type 6, 7 (HHV-6, HSV-7). Results. The HSV1, 2 and EBV genomes were detected in the cornea significantly more often in BCU of prolonged course compared with a favorable course (HSV1, 2 p = 0.012; EBV p = 0.012), and HHV-6 was detected not only in the cornea (p = 0.000), but also and in blood (p = 0.007). In patients with HHV DNA in corneal scarps and/or blood, after resorption of purulent infiltrate, corneal epithelialization was absent, and the use of antiherpetic drugs allowed to reduce the completion time of BCU epithelialization. Conclusion. The role of HHV-6, EBV, HSV 1, 2 in the pathogenesis of bacterial corneal ulcer of protracted course was revealed. The expediency of examination of patients with bacterial corneal ulcer on HHV is shown, a method of treatment is proposed, including antiherpetic therapy, which makes it possible to prevent the development of a protracted course.

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