Genetic characterization of Neospora caninum from aborted bovine fetuses in Hokkaido, Japan

2021 ◽  
pp. 104838
Author(s):  
El-Sayed El-Alfy ◽  
Yuma Ohari ◽  
Naomi Shimoda ◽  
Yoshifumi Nishikawa
2020 ◽  
Vol 280 ◽  
pp. 109091
Author(s):  
Marta García-Sánchez ◽  
Javier Moreno-Gonzalo ◽  
Marta González-Warleta ◽  
Mercedes Mezo ◽  
Luis Miguel Ortega-Mora ◽  
...  

Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 36 ◽  
Author(s):  
Weifeng Qian ◽  
Wenchao Yan ◽  
Chaochao Lv ◽  
Rongzhen Bai ◽  
Tianqi Wang

Currently, information on the occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in tissues of rabbits in China is lacking. In this study, brain and heart samples from 470 slaughtered domestic rabbits were collected in Henan Province, Central China. The occurrence rate of T. gondii and N. caninum DNA detected by nested PCR was 2.8% and 2.1%, respectively. There were no significant differences (p > 0.05) in the frequency of the two parasite infections in relation to sex, breed, and region. Three out of 13 T. gondii-positive samples were completely or partially genotyped at 11 genetic markers using PCR-RFLP, and one was identified as ToxoDB genotype #9. For N. caninum, three different sequences at the ITS1 region and two genotypes at the MS5 microsatellite locus were identified. To our knowledge, this is the first genetic characterization of N. caninum isolates from rabbits.


2020 ◽  
Vol 119 (4) ◽  
pp. 1353-1362 ◽  
Author(s):  
Javier Regidor-Cerrillo ◽  
Pilar Horcajo ◽  
Letizia Ceglie ◽  
Eliana Schiavon ◽  
Luis Miguel Ortega-Mora ◽  
...  

2016 ◽  
Vol 228 ◽  
pp. 183-187 ◽  
Author(s):  
Leticia Medina-Esparza ◽  
Javier Regidor-Cerrillo ◽  
Daniel García-Ramos ◽  
Gema Álvarez-García ◽  
Julio Benavides ◽  
...  

Parasitology ◽  
2008 ◽  
Vol 135 (14) ◽  
pp. 1651-1659 ◽  
Author(s):  
J. REGIDOR-CERRILLO ◽  
M. GÓMEZ-BAUTISTA ◽  
J. PEREIRA-BUENO ◽  
G. ADURIZ ◽  
V. NAVARRO-LOZANO ◽  
...  

SUMMARYNeospora caninum is a cyst-forming parasite that causes abortion in cattle. Despite this parasite's ubiquitous distribution and wide host range, the number of N. caninum isolates obtained to date is limited. In vitro isolation of the parasite is arduous and often unsuccessful. In addition, most isolates have been obtained from clinically affected hosts and therefore could be biased towards more virulent isolates. In this report, an improved isolation approach from transplacentally infected newborn calves was undertaken and 9 new isolates were obtained. Moreover, a microsatellite technique was applied to investigate the genetic diversity of these isolates. Most isolates showed specific genetic profiles. However, the Nc-Spain10 isolate was identical to the previously described Nc-Spain1H isolate and Nc-Spain3H was identical to Nc-Spain4H. These isolates were likely to have identical genotypes because they were isolated from distinct calves of the same herd. Future pathogenic characterization of these isolates will contribute to the investigation of the relationship between isolate virulence and the outcome of infection, as well as other epidemiological features, such as transmission.


2007 ◽  
Vol 149 (3-4) ◽  
pp. 158-166 ◽  
Author(s):  
J.P. Dubey ◽  
M.C.B. Vianna ◽  
O.C.H. Kwok ◽  
D.E. Hill ◽  
K.B. Miska ◽  
...  

2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.


Author(s):  
Rita Indirli ◽  
Biagio Cangiano ◽  
Eriselda Profka ◽  
Elena Castellano ◽  
Giovanni Goggi ◽  
...  

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