Leveraging laboratory and clinical studies to design effective antibiotic combination therapy

2021 ◽  
Vol 64 ◽  
pp. 68-75
Author(s):  
Kathleen Davis ◽  
Talia Greenstein ◽  
Roberto Viau Colindres ◽  
Bree B Aldridge
Keyword(s):  
Combination Therapy ◽  
Clinical Studies ◽  
Antibiotic Combination

scholarly journals Quantification of Fosfomycin in Combination with Nine Antibiotics in Human Plasma and Cation-Adjusted Mueller-Hinton II Broth via LCMS

Antibiotics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 54
Author(s):  
Kelvin Kau-Kiat Goh ◽  
Wilson Ghim-Hon Toh ◽  
Daryl Kim-Hor Hee ◽  
Edwin Zhi-Wei Ting ◽  
Nathalie Grace Sy Chua ◽  
...  
Keyword(s):  
Combination Therapy ◽  
Human Plasma ◽  
Limit Of Detection ◽  
Synergistic Activity ◽  
Limit Of Quantification ◽  
Mueller Hinton ◽  
Antibiotic Drug ◽  
Dosing Regimens ◽  
Antibiotic Combination

Fosfomycin-based combination therapy has emerged as an attractive option in our armamentarium due to its synergistic activity against carbapenem-resistant Gram-negative bacteria (CRGNB). The ability to simultaneously measure fosfomycin and other antibiotic drug levels will support in vitro and clinical investigations to develop rational antibiotic combination dosing regimens against CRGNB infections. We developed an analytical assay to measure fosfomycin with nine important antibiotics in human plasma and cation-adjusted Mueller–Hinton II broth (CAMHB). We employed a liquid-chromatography tandem mass spectrometry method and validated the method based on accuracy, precision, matrix effect, limit-of-detection, limit-of-quantification, specificity, carryover, and short-term and long-term stability on U.S. Food & Drug Administration (FDA) guidelines. Assay feasibility was assessed in a pilot clinical study in four patients on antibiotic combination therapy. Simultaneous quantification of fosfomycin, levofloxacin, meropenem, doripenem, aztreonam, piperacillin/tazobactam, ceftolozane/tazobactam, ceftazidime/avibactam, cefepime, and tigecycline in plasma and CAMHB were achieved within 4.5 min. Precision, accuracy, specificity, and carryover were within FDA guidelines. Fosfomycin combined with any of the nine antibiotics were stable in plasma and CAMHB up to 4 weeks at −80 °C. The assay identified and quantified the respective antibiotics administered in the four subjects. Our assay can be a valuable tool for in vitro and clinical applications.

Triple antibiotic combination therapy may improve but not resolve granuloma annulare

2014 ◽  
Vol 27 (6) ◽  
pp. 343-347 ◽  
Author(s):  
Brenda Simpson ◽  
Shannon Foster ◽  
Jennifer H. Ku ◽  
Eric L. Simpson ◽  
Benjamin D. Ehst
Keyword(s):  
Combination Therapy ◽  
Granuloma Annulare ◽  
Antibiotic Combination

Effectiveness of Antibiotic Combination Therapy Targeting Intestinal Bacteria for Ulcerative Colitis

2015 ◽  
Vol 110 ◽  
pp. S813
Author(s):  
Hiroko Kobayashi ◽  
Shintaro Tsukinaga ◽  
Tomoya Kanai ◽  
Shinichiro Takami ◽  
Keisuke Saito ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Combination Therapy ◽  
Intestinal Bacteria ◽  
Antibiotic Combination

The development of combination therapy involving camptothecins: a review of preclinical and early clinical studies

1998 ◽  
Vol 24 (3) ◽  
pp. 205-220 ◽  
Author(s):  
M.J.A. de Jonge ◽  
A. Sparreboom ◽  
J. Verweij
Keyword(s):  
Combination Therapy ◽  
Clinical Studies
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