Construction of recombinant herpes simplex virus type I expressing green fluorescent protein without loss of any viral genes

2004 ◽  
Vol 6 (5) ◽  
pp. 485-493 ◽  
Author(s):  
M Tanaka
Keyword(s):  
Herpes Simplex Virus ◽  
Green Fluorescent Protein ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Fluorescent Protein ◽  
Type I ◽  
Viral Genes ◽  
Green Fluorescent ◽  
Simplex Virus

Incorporation of green fluorescent protein into the essential envelope glycoprotein B of herpes simplex virus type 1

2002 ◽  
Vol 105 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Corinne Potel ◽  
Karin Kaelin ◽  
Isabelle Gautier ◽  
Pierre Lebon ◽  
Jacques Coppey ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Green Fluorescent Protein ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Envelope Glycoprotein ◽  
Fluorescent Protein ◽  
Green Fluorescent ◽  
Simplex Virus

Structure-function studies of peptides inhibiting the ribonucleotide reductase activity of herpes simplex virus type I

1992 ◽  
Vol 35 (2) ◽  
pp. 346-350 ◽  
Author(s):  
Pierrette Gaudreau ◽  
Paul Brazeau ◽  
Manon Richer ◽  
Jean Cormier ◽  
Daniel Langlois ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Structure Function ◽  
Virus Type ◽  
Ribonucleotide Reductase ◽  
Herpes Simplex Virus Type ◽  
Reductase Activity ◽  
Type I ◽  
Simplex Virus ◽  
Ribonucleotide Reductase Activity

Crystal structures of the thymidine kinase from herpes simplex virus type-I in complex with deoxythymidine and Ganciclovir

1995 ◽  
Vol 2 (10) ◽  
pp. 876-881 ◽  
Author(s):  
D.G. Brown ◽  
R. Visse ◽  
G. Sandhu ◽  
A. Davies ◽  
P.J. Rizkallah ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Crystal Structures ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Type I ◽  
Simplex Virus

scholarly journals Live-Cell Analysis of a Green Fluorescent Protein-Tagged Herpes Simplex Virus Infection

1999 ◽  
Vol 73 (5) ◽  
pp. 4110-4119 ◽  
Author(s):  
Gillian Elliott ◽  
Peter O’Hare
Keyword(s):  
Herpes Simplex Virus ◽  
Green Fluorescent Protein ◽  
Herpes Simplex ◽  
Fluorescent Protein ◽  
Time Lapse ◽  
Live Cell ◽  
Cell Periphery ◽  
Green Fluorescent ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT Many stages of the herpes simplex virus maturation pathway have not yet been defined. In particular, little is known about the assembly of the virion tegument compartment and its subsequent incorporation into maturing virus particles. Here we describe the construction of a herpes simplex virus type 1 (HSV-1) recombinant in which we have replaced the gene encoding a major tegument protein, VP22, with a gene expressing a green fluorescent protein (GFP)-VP22 fusion protein (GFP-22). We show that this virus has growth properties identical to those of the parental virus and that newly synthesized GFP-22 is detectable in live cells as early as 3 h postinfection. Moreover, we show that GFP-22 is incorporated into the HSV-1 virion as efficiently as VP22, resulting in particles which are visible by fluorescence microscopy. Consequently, we have used time lapse confocal microscopy to monitor GFP-22 in live-cell infection, and we present time lapse animations of GFP-22 localization throughout the virus life cycle. These animations demonstrate that GFP-22 is present in a diffuse cytoplasmic location when it is initially expressed but evolves into particulate material which travels through an exclusively cytoplasmic pathway to the cell periphery. In this way, we have for the first time visualized the trafficking of a herpesvirus structural component within live, infected cells.

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