Identification and characterization of novel natural pathogen of Drosophila melanogaster isolated from wild captured Drosophila spp.

2016 ◽  
Vol 18 (12) ◽  
pp. 813-821 ◽  
Author(s):  
Karan Singh ◽  
Mohammad Zulkifli ◽  
N.G. Prasad
Keyword(s):  
Drosophila Melanogaster ◽  
Identification And Characterization

scholarly journals Identification and characterization of DAlk: a novel Drosophila melanogaster RTK which drives ERK activation in vivo

2001 ◽  
Vol 6 (6) ◽  
pp. 531-544 ◽  
Author(s):  
Christina E. Lorén ◽  
Audra Scully ◽  
Caroline Grabbe ◽  
Philip T. Edeen ◽  
John Thomas ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Erk Activation ◽  
Identification And Characterization

scholarly journals Characterization of TbPDE2A, a Novel Cyclic Nucleotide-specific Phosphodiesterase from the Protozoan ParasiteTrypanosoma brucei

2000 ◽  
Vol 276 (15) ◽  
pp. 11559-11566 ◽  
Author(s):  
Roya Zoraghi ◽  
Stefan Kunz ◽  
Kewei Gong ◽  
Thomas Seebeck
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Drosophila Melanogaster ◽  
Trypanosoma Brucei ◽  
Dictyostelium Discoideum ◽  
Cyclic Nucleotide ◽  
Catalytic Domain ◽  
Sequence Identity ◽  
Essential Enzyme ◽  
Identification And Characterization

This study reports the identification and characterization of a cAMP-specific phosphodiesterase from the parasitic hemoflagellateTrypanosoma brucei. TbPDE2A is a class I phosphodiesterase. Its catalytic domain exhibits 30–40% sequence identity with those of all 11 mammalian phosphodiesterase (PDE) families, as well as withPDE2fromSaccharomyces cerevisiae,duncefromDrosophila melanogaster, andregAfromDictyostelium discoideum. The overall structure of TbPDE2A resembles that of human PDE11A in that its N-terminal region contains a single GAF domain. This domain is very similar to those of the mammalian PDE2, -5, -6, -10, and -11, where it constitutes a potential cGMP binding site. TbPDE2A can be expressed inS. cerevisiae, and it complements anS. cerevisiaePDE deletion strain. Recombinant TbPDE2A is specific for cAMP, with aKmof ∼2 μm. It is entirely resistant to the nonselective PDE inhibitor 3-isobutyl-1-methylxanthine, but it is sensitive to trequinsin, dipyridamole, sildenafil, and ethaverine with IC50values of 5.4, 5.9, 9.4, and 14.2 μm, respectively. All four compounds inhibit proliferation of bloodstream form trypanosomes in culture, indicating that TbPDE2A is an essential enzyme.

scholarly journals Identification and characterization of a G protein-coupled receptor for the neuropeptide proctolin in Drosophila melanogaster

2003 ◽  
Vol 100 (10) ◽  
pp. 6198-6203 ◽  
Author(s):  
E. C. Johnson ◽  
S. F. Garczynski ◽  
D. Park ◽  
J. W. Crim ◽  
D. R. Nassel ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
G Protein ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled ◽  
Identification And Characterization
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