scholarly journals Sensitive and specific detection of Xanthomonas campestris pv. vesicatoria by PCR using pathovar-specific primers based on rhs family gene sequences

2009 ◽  
Vol 164 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Dong Suk Park ◽  
Jae Kyung Shim ◽  
Jung Sun Kim ◽  
Chun Keun Lim ◽  
Rosemary Shrestha ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Gene Sequences ◽  
Specific Detection ◽  
Specific Primers ◽  
Family Gene

scholarly journals Sensitive and specific detection of Xanthomonas axonopodis pv. citri by PCR using pathovar specific primers based on hrpW gene sequences

2006 ◽  
Vol 161 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Dong Suk Park ◽  
Jae Wook Hyun ◽  
Young Jin Park ◽  
Jung Sun Kim ◽  
Hee Wan Kang ◽  
...  
Keyword(s):  
Gene Sequences ◽  
Specific Detection ◽  
Specific Primers ◽  
Xanthomonas Axonopodis

Sensitive and specific detection of Xanthomonas campestris pv. zinniae by PCR using pathovar-specific primers

2019 ◽  
Vol 156 (2) ◽  
pp. 491-500
Author(s):  
Yu-ting Zhao ◽  
George W. Sundin ◽  
Xiao-yan Zhang ◽  
Bao-hui Lu ◽  
Jie Gao
Keyword(s):  
Xanthomonas Campestris ◽  
Specific Detection ◽  
Specific Primers

scholarly journals PCR-based sensitive and specific detection of Pectobacterium atrosepticum using primers based on Rhs family gene sequences

2006 ◽  
Vol 55 (5) ◽  
pp. 625-629 ◽  
Author(s):  
D. S. Park ◽  
J. K. Shim ◽  
J. S. Kim ◽  
B. Y. Kim ◽  
M. J. Kang ◽  
...  
Keyword(s):  
Gene Sequences ◽  
Specific Detection ◽  
Pectobacterium Atrosepticum ◽  
Family Gene

scholarly journals The Termite Group I Phylum Is Highly Diverse and Widespread in the Environment

2007 ◽  
Vol 73 (20) ◽  
pp. 6682-6685 ◽  
Author(s):  
Daniel P. R. Herlemann ◽  
Oliver Geissinger ◽  
Andreas Brune
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Specific Primers ◽  
Environmental Distribution ◽  
Data Set ◽  
Group I

ABSTRACT The bacterial candidate phylum Termite Group I (TG-1) presently consists mostly of “Endomicrobia,” which are endosymbionts of flagellate protists occurring exclusively in the hindguts of termites and wood-feeding cockroaches. Here, we show that public databases contain many, mostly undocumented 16S rRNA gene sequences from other habitats that are affiliated with the TG-1 phylum but are only distantly related to “Endomicrobia.” Phylogenetic analysis of the expanded data set revealed several diverse and deeply branching lineages comprising clones from many different habitats. In addition, we designed specific primers to explore the diversity and environmental distribution of bacteria in the TG-1 phylum.

scholarly journals Specific Detection of Arcobacter and Campylobacter Strains in Water and Sewage by PCR and Fluorescent In Situ Hybridization

2003 ◽  
Vol 69 (2) ◽  
pp. 1181-1186 ◽  
Author(s):  
Yolanda Moreno ◽  
Salut Botella ◽  
José Luis Alonso ◽  
María A. Ferrús ◽  
Manuel Hernández ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Sequence Data ◽  
23S Rrna ◽  
Specific Detection ◽  
Specific Primers ◽  
Detection Range ◽  
Water And Wastewater ◽  
Wastewater Samples

ABSTRACT The aim of this study was to evaluate PCR and fluorescent in situ hybridization (FISH) techniques for detecting Arcobacter and Campylobacter strains in river water and wastewater samples. Both 16S and 23S rRNA sequence data were used to design specific primers and oligonucleotide probes for PCR and FISH analyses, respectively. In order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on selective media. The detection range of PCR and FISH assays varied between 1 cell/ml (after enrichment) to 103 cells/ml (without enrichment). According to our results, both rRNA-based techniques have the potential to be used as quick and sensitive methods for detection of campylobacters in environmental samples.

scholarly journals Genus-Specific Detection of Salmonellae using the Polymerase Chain Reaction (PCR)

1993 ◽  
Vol 5 (3) ◽  
pp. 368-371 ◽  
Author(s):  
Noah D. Cohen ◽  
Holly L. Neibergs ◽  
Edward D. McGruder ◽  
Howard W. Whitford ◽  
Robert W. Behle ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Anaerobic Bacteria ◽  
Specific Detection ◽  
Chain Reaction ◽  
Specific Primers ◽  
Genetic Sequence ◽  
Negative Results ◽  
The Family ◽  
Polymerase Chain ◽  
Aerobic And Anaerobic

Oligonucleotide primers for the polymerase chain reaction (PCR) that enable genus-specific detection of members of the genus Salmonella were developed. The primers amplify a 496-bp genetic sequence of members of the genus Salmonella. Amplification of DNA extracted from all other genera of the family Enterobacteriaceae and various other gram-positive aerobic and anaerobic bacteria yielded negative results. Applications of the PCR using these genus-specific primers are discussed.

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