Genus-Specific Detection of Salmonellae using the Polymerase Chain Reaction (PCR)

Oligonucleotide primers for the polymerase chain reaction (PCR) that enable genus-specific detection of members of the genus Salmonella were developed. The primers amplify a 496-bp genetic sequence of members of the genus Salmonella. Amplification of DNA extracted from all other genera of the family Enterobacteriaceae and various other gram-positive aerobic and anaerobic bacteria yielded negative results. Applications of the PCR using these genus-specific primers are discussed.

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PCR Assay With Host Specific Internal Control for Staphylococcus Aureus From Bovine Milk Samples

Macedonian Veterinary Review ◽

10.14432/j.macvetrev.2015.01.038 ◽

2015 ◽

Vol 38 (1) ◽

pp. 97-100 ◽

Cited By ~ 1

Author(s):

Zafer Cantekin ◽

Yasar Ergun ◽

Hasan Solmaz ◽

Gamze Özge Özmen ◽

Melek Demir ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Polymerase Chain Reaction ◽

False Negative ◽

Bovine Milk ◽

Chain Reaction ◽

Specific Primers ◽

Milk Samples ◽

Negative Results ◽

False Negative Results ◽

Polymerase Chain

Abstract Staphylococcus aureus is considered as one of the most important and common pathogens of bovine mastitis. Polymerase Chain Reaction is frequently proposed in the diagnosis of S. aureus directly from milk samples instead of classical culture. However, false-negative results may occur in the polymerase chain reaction analysis performed directly from clinical material. For the purpose of disclosing the false negative results, the use of internal amplification controls can be beneficial. Therefore, in this study a new polymerase chain reaction technique with host specific internal amplification control was developed by optimizing S. aureus-specific primers in combination with bovine specific primers. The effectiveness of the developed technique in this study was attempted in milk samples from bovine subclinical mastitis. This technique has the potential to detect S. aureus from bovine milk samples or dairy products.

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High-resolution HLA-DQB1 typing using the polymerase chain reaction and sequence-specific primers

Tissue Antigens ◽

10.1111/j.1399-0039.1997.tb02936.x ◽

1997 ◽

Vol 50 (6) ◽

pp. 688-692 ◽

Cited By ~ 14

Author(s):

C.G. Mullighan ◽

M. Bunce ◽

K.I. Welsh

Keyword(s):

Polymerase Chain Reaction ◽

High Resolution ◽

Chain Reaction ◽

Specific Primers ◽

Polymerase Chain

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A msp1α polymerase chain reaction assay for specific detection and differentiation of Anaplasma marginale isolates

Veterinary Microbiology ◽

10.1016/s0378-1135(02)00017-2 ◽

2002 ◽

Vol 86 (4) ◽

pp. 325-335 ◽

Cited By ~ 55

Author(s):

A.E. Lew ◽

R.E. Bock ◽

C.M. Minchin ◽

S. Masaka

Keyword(s):

Polymerase Chain Reaction ◽

Polymerase Chain Reaction Assay ◽

Anaplasma Marginale ◽

Specific Detection ◽

Chain Reaction ◽

Polymerase Chain

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Sensitive and semi-quantitative TaqMan™ real-time polymerase chain reaction systems for the detection of beef (Bos taurus) and the detection of the family Mammalia in food and feed

Meat Science ◽

10.1016/s0309-1740(02)00253-x ◽

2003 ◽

Vol 65 (1) ◽

pp. 599-607 ◽

Cited By ~ 94

Author(s):

Peter D. Brodmann ◽

Dominik Moor

Keyword(s):

Polymerase Chain Reaction ◽

Real Time ◽

Bos Taurus ◽

Chain Reaction ◽

Reaction Systems ◽

The Family ◽

Food And Feed ◽

Polymerase Chain

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A rapid molecular method (polymerase chain reaction with sequence-specific primers) to genotype for ABO blood group and secretor status and its potential for organ transplants

Tissue Antigens ◽

10.1111/j.1399-0039.1997.tb02902.x ◽

1997 ◽

Vol 50 (5) ◽

pp. 475-483 ◽

Cited By ~ 27

Author(s):

J. Procter ◽

J. Crawford ◽

M. Bunce ◽

K.I. Welsh

Keyword(s):

Polymerase Chain Reaction ◽

Blood Group ◽

Abo Blood Group ◽

Organ Transplants ◽

Molecular Method ◽

Chain Reaction ◽

Specific Primers ◽

Secretor Status ◽

Polymerase Chain

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Amplification of human beta-actin gene by the reverse transcriptase-polymerase chain reaction: implications for assessment of RNA from formalin-fixed, paraffin-embedded material.

Journal of Histochemistry & Cytochemistry ◽

10.1177/44.10.8813086 ◽

1996 ◽

Vol 44 (10) ◽

pp. 1205-1207 ◽

Cited By ~ 18

Author(s):

A Dakhama ◽

V Macek ◽

J C Hogg ◽

R G Hegele

Keyword(s):

Polymerase Chain Reaction ◽

Pcr Amplification ◽

Housekeeping Gene ◽

Chain Reaction ◽

Viral Genomes ◽

Negative Results ◽

Beta Actin ◽

Target Nucleic Acid ◽

Formalin Fixed Paraffin Embedded ◽

Polymerase Chain

The polymerase chain reaction (PCR) is a powerful method that allows enzymatic amplification of rate target nucleic acid sequences. It has been applied to the amplification of viral genomes from paraffin-embedded pathology specimens. However, interpretation of negative results requires amplification of a housekeeping gene such as beta-actin. In the present study we used specific oligonucleotide primers previously designed to amplify both the genomic DNA and the mRNA transcript from paraffin-embedded tissue. These products have predicted sizes of 250 BP and 154 BP, respectively, but our results showed that PCR amplification only (without reverse transcription) unexpectedly generated the 154-BP product. Further investigation of the nature of this product demonstrated that it originated from the amplification of DNA, not RNA. We conclude that the 154-BP product generated by these primers cannot be exclusively considered as beta-actin RNA product and should not be used to assess successful extraction of RNA, to ascertain its integrity, or to normalize for the total amount of RNA assayed by RT-PCR from paraffin-embedded tissue.

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UTILITY OF ANTIGEN DETECTION TEST AND POLYMERASE CHAIN REACTION IN THE DIFFERENTIATION OF TUBERCULOUS AND NON-TUBERCULOUS MYCOBACTERIA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i6.18261 ◽

2017 ◽

Vol 10 (6) ◽

pp. 289

Author(s):

Yogita Singh ◽

Raji Vasanth ◽

Shrikala Baliga ◽

Dhanashree B

Keyword(s):

Polymerase Chain Reaction ◽

False Negative ◽

Diagnostic Methods ◽

Clinical Samples ◽

Chain Reaction ◽

College Hospital ◽

Medical College ◽

Negative Results ◽

Polymerase Chain ◽

Mycobacterium Spp

Objectives: Cultivation and identification of mycobacteria to species level remains difficult and time-consuming. Hence, easy and rapid diagnostic methods are necessary for the differentiation of Mycobacterium tuberculosis (MTB) from non-tuberculous mycobacteria (NTM). The present study aims to detect and differentiate MTB from NTM isolated from clinical samples by immunochromatographic test (ICT) and polymerase chain reaction (PCR). Methods: Over a period of 1 year, clinical samples (n=496) received from suspected cases of TB, at the Department of Microbiology, Kasturba Medical College Hospital, Mangalore were cultured to isolate Mycobacterium spp. Identification of all the isolates was done by conventional biochemical technique, ICT, and PCR. Results: Among the 496 samples processed, 49 (9.87%) were acid-fast bacilli smear positive and 59 (11.89%) samples showed the growth of Mycobacterium spp. Among these, 10 were rapid growers, 49 were slow-growing mycobacteria, out of which 30 were MTB as identified by conventional biochemical reaction. Out of 59 Mycobacterial isolates subjected to ICT for the detection of MPT 64 antigen, only 28 were identified as MTB. However, all the 30 isolates were correctly identified as MTB by PCR. Conclusion: Hence, PCR is essential for rapid differentiation of non-tuberculous Mycobacterium from MTB. False negative results seen with immunochromatographic MPT 64 antigen assay could be due to mutations within the mpt64 gene. Further studies are necessary to characterize these PCR-positive and immunochromatographic assay negative MTB isolates.

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