Salt-induced homogenous solid phase extraction of hydroxylated metabolites of polycyclic aromatic hydrocarbons from urine samples using a deep eutectic solvent as an elution solvent prior to HPLC-FLD analysis

A diatomite supported graphene oxide composite (GO@Dt–NH2) was fabricated and explored as a solid-phase extraction adsorbent coupled with high performance liquid chromatography to determine the trace hydroxyl polycyclic aromatic hydrocarbons (2-hydroxy-naphthalene, 2-hydroxy-fluorene, 1-hydroxy-phenanthrene, and 1-hydroxy-pyrene) in urine samples. The fabricated composites were characterized by X-ray powder diffractometry and scanning electron microscopy. GO@Dt–NH2 offered enhanced adsorption affinity towards the analytes compared with the bare diatomite. The amount of graphene oxide and the factors affecting solid-phase extraction were investigated in detail. Under the optimized conditions, the method gave good linearity (0.30–200 ng/mL) and a low detection limit (0.10–0.15 ng/mL) for the hydroxyl polycyclic aromatic hydrocarbons. The average recovery for spiked urine samples with three levels ranged from 90.6% to 100%. The intra-day and inter-day relative standard deviations were in the range of 1.8–6.4% and 2.7–11.8%, respectively. Besides, the GO@Dt–NH2 provided enrichment factors of 18–20 and superior purification ability. The developed method was successfully applied to the determination of hydroxyl polycyclic aromatic hydrocarbons in urine samples from smoking volunteers.

Download Full-text

Automated micro‐solid‐phase extraction clean‐up of polycyclic aromatic hydrocarbons in food oils for analysis by gas chromatography–orbital ion trap mass spectrometry

Journal of Separation Science ◽

10.1002/jssc.202000720 ◽

2020 ◽

Author(s):

Philipp Eyring ◽

Thomi Preiswerk ◽

Henrik Lauritz Frandsen ◽

Lene Duedahl‐Olesen

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Polycyclic Aromatic Hydrocarbons ◽

Solid Phase Extraction ◽

Aromatic Hydrocarbons ◽

Ion Trap ◽

Solid Phase ◽

Ion Trap Mass Spectrometry ◽

Phase Extraction ◽

Polycyclic Aromatic

Download Full-text

A Correlation Study between Polycyclic Aromatic Hydrocarbons in Urumqi Snow and the Surrounding Environment

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.864-867.1364 ◽

2013 ◽

Vol 864-867 ◽

pp. 1364-1368

Author(s):

Yakup Parida ◽

Eslamjan Diwani

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Solid Phase Extraction ◽

Aromatic Hydrocarbons ◽

Solid Phase ◽

Total Concentration ◽

Correlation Study ◽

Phase Extraction ◽

Purification Method ◽

Surrounding Environment ◽

Polycyclic Aromatic

Based on the principle of solid phase extraction and the purification method using Florida silica, sixteen various kinds of polycyclic aromatic hydrocarbons such as naphthalene, acenaphthene and fluorene were measured in Urumqi snow by means of HPLC. The results showed that the concentration of PAHs was different in different sampling sites in Urumqi. The total concentration of PAHs was in the range of 0.2883 ~ 8.814 µg/mL and the content of PAHs snow was higher than that in water, which indicated that the snow was polluted by organic matters to a certain degree and the pollution was not so serious compared with the groundwater. So the snow has a certain application value for life.

Download Full-text