Immobilized penicillin G acylase on mesoporous silica: The influence of pore size, pore volume and mesophases

2008 ◽  
Vol 114 (1-3) ◽  
pp. 507-510 ◽  
Author(s):  
Yongjun Lü ◽  
Yanglong Guo ◽  
Yanqin Wang ◽  
Xiaohui Liu ◽  
Yunsong Wang ◽  
...  
2000 ◽  
Vol 18 (3) ◽  
pp. 253-258 ◽  
Author(s):  
Gregorio Alvaro ◽  
Josep A. Feliu ◽  
Gloria Caminal ◽  
Josep López-santín ◽  
Pere Clapés

2012 ◽  
Vol 550-553 ◽  
pp. 1345-1351 ◽  
Author(s):  
Wei Wang ◽  
Zhong Hai Li ◽  
Ji Lie Li ◽  
Yao Hui Wu

Magnetic hydroxyl microspheres were prepared by suspension polymerization and activated by epoxy chloropropane. The magnetic particles were characterized in terms of chemical composition, particle size and electrophoretic mobility. These epoxy-activated magnetic particles were assessed as a new carries for immobilized penicillin G acylase (PGA) by covalent coupling. The oriented PGA immobilization was achieved by employing the interaction electrostatic repulsion between PGA and magnetic supports through adding the phenyl acetic acid (PAA), which resulted in a 1.194-fold increase in the enzyme activity yield as compared to that of untreated PGA. No activity of immobilized PGA was lost after 20 cycles and about 94.28% enzyme activity was retained at the end of the 80th cycle in the batch reaction system.


2014 ◽  
Vol 35 (10) ◽  
pp. 1709-1715 ◽  
Author(s):  
Wangcheng Zhan ◽  
Yongjun Lü ◽  
Ling Yang ◽  
Yanglong Guo ◽  
Yanqin Wang ◽  
...  

2017 ◽  
Vol 15 (1) ◽  
pp. 60
Author(s):  
Nasri Nasri ◽  
R R Dirgarini J. N Subagyono ◽  
Rahmat Gunawan

The synthesis and characterization of  mesoporous silica Santa Barbara Acid-15 (SBA-15) from the ash of corn have been done.  SBA-15 was synthesised using the surfactant template method. SBA-15 from corn leaves  ash has a surface area of 592 m2 / gram, pore volume of 0.99 cm3 / gram and pore diameter of 3.95 nm with pore size ranging from 4.9 nm - 7.1 nm. The SAXS pattern of the bio-SBA-15 showed three typical peaks of SBA-15 with miller indices [100], [110] and [200]. In the FTIR spectrum of  SBA-15, peaks from Si-O-Si, Si-OH and H-O-H from water were observed.  


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