Evaluation of five commercial methods for the extraction and purification of DNA from human faecal samples for downstream molecular detection of the enteric protozoan parasites Cryptosporidium spp., Giardia duodenalis, and Entamoeba spp

2016 ◽  
Vol 127 ◽  
pp. 68-73 ◽  
Author(s):  
Silvia Paulos ◽  
Marta Mateo ◽  
Aida de Lucio ◽  
Marta Hernández-de Mingo ◽  
Begoña Bailo ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Giardia Duodenalis ◽  
Protozoan Parasites ◽  
Cryptosporidium Spp ◽  
Extraction And Purification ◽  
Faecal Samples ◽  
Enteric Protozoan

Molecular detection of Giardia duodenalis and Cryptosporidium spp. in canine faecal samples contaminating public areas in Northern Italy

2017 ◽  
Vol 116 (12) ◽  
pp. 3411-3418 ◽  
Author(s):  
Giulia Simonato ◽  
Antonio Frangipane di Regalbono ◽  
Rudi Cassini ◽  
Donato Traversa ◽  
Cinzia Tessarin ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Giardia Duodenalis ◽  
Northern Italy ◽  
Cryptosporidium Spp ◽  
Faecal Samples ◽  
Public Areas

scholarly journals Prevalence of intestinal parasites in children from minority group with low hygienic standards in Slovakia

2012 ◽  
Vol 49 (2) ◽  
pp. 63-66 ◽  
Author(s):  
P. Rudohradská ◽  
M. Halánová ◽  
P. Ravaszová ◽  
M. Goldová ◽  
A. Valenčáková ◽  
...  
Keyword(s):  
Socioeconomic Status ◽  
Human Population ◽  
Intestinal Parasites ◽  
Minority Group ◽  
Developed Countries ◽  
Giardia Intestinalis ◽  
Protozoan Parasites ◽  
Cryptosporidium Spp ◽  
Faecal Samples ◽  
Hygienic Standard

AbstractThe number of parasites followed the rapid growing of human population worldwide, not only in developing but also in developed countries. Many of them are diagnosed in children and adolescents. The occurrence of selected intestinal endoparasites in children coming from areas with low hygienic and socioeconomic status was studied. Out of 81 faecal samples examined, 46 (56.8 %) were positive for presence of intestinal parasites. From helminths, Ascaris lumbricoides was found to be the leading parasite (24.7 %), followed by Trichuris trichiura (17.3 %). Tapeworm Taenia spp. eggs were detected in 4.9 % of examined children. From protozoan parasites Cryptosporidium spp. was observed in 36 children (44.4 %) and Giardia intestinalis in 20 children (24.7 %). The occurrence of these epidemiologically low risky parasites in Roma children population suggests low hygienic standard in the Roma settlements.

Molecular detection of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in school children at the Thai-Myanmar border

2021 ◽  
Author(s):  
Chantira Sutthikornchai ◽  
Supaluk Popruk ◽  
Aongart Mahittikorn ◽  
Dumrongkiet Arthan ◽  
Ngamphol Soonthornworasiri ◽  
...  
Keyword(s):  
School Children ◽  
Molecular Detection ◽  
Giardia Duodenalis ◽  
Enterocytozoon Bieneusi ◽  
Cryptosporidium Spp

Molecular detection and prevalence of Giardia duodenalis and Cryptosporidium spp. among long-tailed macaques (Macaca fascicularis) in Thailand

2016 ◽  
Vol 40 ◽  
pp. 310-314 ◽  
Author(s):  
Wanat Sricharern ◽  
Tawin Inpankaew ◽  
Sarawan Keawmongkol ◽  
Juthamas Supanam ◽  
Roger W. Stich ◽  
...  
Keyword(s):  
Macaca Fascicularis ◽  
Molecular Detection ◽  
Giardia Duodenalis ◽  
Cryptosporidium Spp

scholarly journals Prevalence of Cryptosporidium spp. and Giardia duodenalis in pigs in Lusaka, Zambia

2012 ◽  
Vol 79 (1) ◽  
Author(s):  
Joyce Siwila ◽  
Kabemba E. Mwape
Keyword(s):  
Age Groups ◽  
Giardia Duodenalis ◽  
Immunofluorescence Assay ◽  
Management Systems ◽  
Intensive Management ◽  
Cryptosporidium Spp ◽  
Source Of Infection ◽  
Faecal Samples ◽  
Study Results ◽  
Faecal Consistency

This study was aimed at determining the prevalence of Cryptosporidium spp. and Giardia duodenalis in pigs which were being raised in intensive management systems. Faecal samples were collected from pigs of all age groups from three different piggery units. Samples were collected directly from the rectum for piglets and weaners and from the floor within 2 min – 5 min of excretion for sows and boars. At the time of collection, faecal consistency was noted as being normal, pasty or diarrhoeic. Samples were analysed further using the Merifluor® Cryptosporidium/Giardia immunofluorescence assay. All piggeries had at least one pig infected with either parasite. From a total 217 samples collected, 96 (44.2%; confidence interval [CI] = 37.6% – 50.9%) were positive for Cryptosporidium spp., whilst 26 (12%; CI = 7.6% – 16.3%) had G. duodenalis parasites. Of all the pigs, 6.9% (15/217) harboured both parasites. With regard to Cryptosporidium spp. infection, statistically significant differences were observed amongst the three units (p = 0.001), whereas no significant differences were observed for G. duodenalis infection (p = 0.13). Prevalence was higher in weaners as compared to other pig classes for both parasites, with significant differences being observed for G. duodenalis infection (p = 0.013). There was, however, no difference in infection between male and female pigs for both parasites. Furthermore, most infections were asymptomatic. From the study results it was clear that Cryptosporidium spp. and G. duodenalis infections were prevalent amongst pigs in the piggeries evaluated and, as such, may act as a source of infection for persons who come into contact with them.

scholarly journals Molecular diversity and frequency of the diarrheagenic enteric protozoan Giardia duodenalis and Cryptosporidium spp. in a hospital setting in Northern Spain

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0178575 ◽  
Author(s):  
José Manuel Azcona-Gutiérrez ◽  
Aida de Lucio ◽  
Marta Hernández-de-Mingo ◽  
Concepción García-García ◽  
Luis Miguel Soria-Blanco ◽  
...  
Keyword(s):  
Molecular Diversity ◽  
Hospital Setting ◽  
Giardia Duodenalis ◽  
Northern Spain ◽  
Cryptosporidium Spp ◽  
Enteric Protozoan

scholarly journals Ethanol and Isopropanol in Concentrations Present in Hand Sanitizers Sharply Reduce Excystation of Giardia and Entamoeba and Eliminate Oral Infectivity of Giardia Cysts in Gerbils

2015 ◽  
Vol 59 (11) ◽  
pp. 6749-6754 ◽  
Author(s):  
Aparajita Chatterjee ◽  
Giulia Bandini ◽  
Edwin Motari ◽  
John Samuelson
Keyword(s):  
Giardia Duodenalis ◽  
Single Layer ◽  
Oral Route ◽  
Protozoan Parasites ◽  
Content Type ◽  
Hand Sanitizer ◽  
Amebic Dysentery ◽  
Entamoeba Invadens ◽  
Enteric Protozoan

ABSTRACTEnteric protozoan parasites, which are spread by the fecal-oral route, are important causes of diarrhea (Giardia duodenalis) and amebic dysentery (Entamoeba histolytica). Cyst walls ofGiardiaandEntamoebahave a single layer composed of fibrils of β-1,3-linked GalNAc and β-1,4-linked GlcNAc (chitin), respectively. The goal here was to determine whether hand sanitizers that contain ethanol or isopropanol as the active microbicide might reduce transmission of these parasites. We found that treatment with these alcohols with or without drying in a rotary evaporator (to model rapid evaporation of sanitizers on hands) kills 85 to 100% of cysts ofG. duodenalisand 90 to 100% of cysts ofEntamoeba invadens(a nonpathogenic model forE. histolytica), as shown by nuclear labeling with propidium iodide and failure to excystin vitro. Alcohols with or without drying collapsed the cyst walls ofGiardiabut did not collapse the cyst walls ofEntamoeba. To validate thein vitroresults, we showed that treatment with alcohols eliminated oral infection of gerbils by 1,000G. duodenaliscysts, while a commercial hand sanitizer (Purell) killedE. invadenscysts that were directly applied to the hands. These results suggest that expanded use of alcohol-based hand sanitizers might reduce the transmission ofGiardiaandEntamoeba.

scholarly journals Cryptosporidium spp., Giardia duodenalis and Toxoplasma gondii detection in fresh vegetables consumed in Marrakech, Morocco

2020 ◽  
Vol 20 (4) ◽  
pp. 1669-78
Author(s):  
Salma Berrouch ◽  
Sandie Escotte-Binet ◽  
Yassine Amraouza ◽  
Pierre Flori ◽  
Dominique Aubert ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Giardia Duodenalis ◽  
Epidemiological Data ◽  
Contaminated Water ◽  
Protozoan Parasites ◽  
Leafy Greens ◽  
Cryptosporidium Spp ◽  
Vegetable Samples ◽  
Fresh Vegetables

Background: Protozoan parasites such as Toxoplasma gondii, Giardia duodenalis, and Cryptosporidium spp., can be transmitted to humans via accidental consumption of contaminated water, fresh produce and foodstuffs. There is a lack of epidemiological data about these pathogens in Morocco. Hence the aim of this study, which is the determination of their prevalence in some leafy greens and root vegetables sold in Marrakech. Methods: A total of 132 vegetable samples including carrot, coriander, lettuce, parsley and radish were purchased monthly from three different markets in Marrakech from March 2017 to January 2018, pre-treated and subjected to microscopic and molecular analyses. Results: Of the 132 samples of vegetables analyzed by qPCR, the overall rate of protozoan was 21.21% (28/132); 22 samples were found to be contaminated with T. gondii, 6 with G. duodenalis, and none was positive for C. parvum/hominis. Whereas, modified Ziehl-Neelsen staining allowed the detection of Cryptosporidium spp. in 3% (4/132) of examined samples. Conclusion: This survey on the presence of protozoan parasites in fresh vegetables revealed that vegetables sold in Marrakech are contaminated by these protozoan parasites, as it showed that leafy green vegetables were more susceptible for parasitic contamination than root ones. Keywords: Fresh vegetables; protozoan parasites; Marrakech; qPCR.

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