scholarly journals Toward a Comprehensive Temperature-Sensitive Mutant Repository of the Essential Genes of Saccharomyces cerevisiae

2008 ◽  
Vol 30 (2) ◽  
pp. 248-258 ◽  
Author(s):  
Shay Ben-Aroya ◽  
Candice Coombes ◽  
Teresa Kwok ◽  
Kathryn A. O'Donnell ◽  
Jef D. Boeke ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Essential Genes ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant

Putative GTPase Gtr1p genetically interacts with the RanGTPase cycle in Saccharomyces cerevisiae

1996 ◽  
Vol 109 (9) ◽  
pp. 2311-2318 ◽  
Author(s):  
N. Nakashima ◽  
N. Hayashi ◽  
E. Noguchi ◽  
T. Nishimoto
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Negative Regulator ◽  
Temperature Sensitive ◽  
Nucleotide Exchange ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Importin Alpha ◽  
Nucleotide Exchange Factor ◽  
Cold Sensitive ◽  
Gtpase Cycle

In order to identify a protein interacting with RCC1, a guanine nucleotide-exchange factor for the nuclear GTPase Ran, we isolated a series of cold-sensitive suppressors of mtr1-2, a temperature-sensitive mutant of the Saccharomyces cerevisiae RCC1 homologue. One of the isolated suppressor mutants was mutated in the putative GTPase Gtr1p, being designated as gtr1-11. It also suppressed other alleles of mtr1-2, srm1-1 and prp20-1 in contrast to overexpression of the S. cerevisiae Ran/TC4 homologue Gsp1p, previously reported to suppress prp20-1, but not mtr1-2 or srm1-1. Furthermore, gtr1-11 suppressed the rna1-1, temperature-sensitive mutant of the Gsp1p GTPase-activating protein, but not the srp1-31, temperature-sensitive mutant of the S. cerevisiae importin alpha homologue. mtr1-2, srm1-1 and prp20-1 were also suppressed by overexpression of the mutated Gtr1p, Gtr1-11p. In summary, Gtr1p that was localized in the cytoplasm by immunofluoresence staining was suggested to function as a negative regulator for the Ran/TC4 GTPase cycle.

scholarly journals A METHOD FOR OBTAINING ANTIBIOTIC-SENSITIVE MUTANTS IN SACCHAROMYCES CEREVISIAE

Genetics ◽  
1972 ◽  
Vol 71 (2) ◽  
pp. 305-308
Author(s):  
Barbara S Littlewood
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant

ABSTRACT We have isolated a temperature-sensitive mutant of S. cerevisiae which has proven useful for the isolation of antibiotic-sensitive strains.

scholarly journals Identification of an altered elongation factor in temperature-sensitive mutant ts 7'-14 of Saccharomyces cerevisiae.

1984 ◽  
Vol 259 (23) ◽  
pp. 14347-14349 ◽  
Author(s):  
F Herrera ◽  
J A Martinez ◽  
N Moreno ◽  
I Sadnik ◽  
C S McLaughlin ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Elongation Factor ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant
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