Studies on nuclear and mitochondrial DNA-replication in a temperature-sensitive mutant of Saccharomyces cerevisiae

1974 ◽  
Vol 131 (4) ◽  
pp. 291-299 ◽  
Author(s):  
Ulrike Wintersberger ◽  
Jennifer Hirsch ◽  
Anne Michelle Fink
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mitochondrial Dna ◽  
Dna Replication ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Mitochondrial Dna Replication

Putative GTPase Gtr1p genetically interacts with the RanGTPase cycle in Saccharomyces cerevisiae

1996 ◽  
Vol 109 (9) ◽  
pp. 2311-2318 ◽  
Author(s):  
N. Nakashima ◽  
N. Hayashi ◽  
E. Noguchi ◽  
T. Nishimoto
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Negative Regulator ◽  
Temperature Sensitive ◽  
Nucleotide Exchange ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Importin Alpha ◽  
Nucleotide Exchange Factor ◽  
Cold Sensitive ◽  
Gtpase Cycle

In order to identify a protein interacting with RCC1, a guanine nucleotide-exchange factor for the nuclear GTPase Ran, we isolated a series of cold-sensitive suppressors of mtr1-2, a temperature-sensitive mutant of the Saccharomyces cerevisiae RCC1 homologue. One of the isolated suppressor mutants was mutated in the putative GTPase Gtr1p, being designated as gtr1-11. It also suppressed other alleles of mtr1-2, srm1-1 and prp20-1 in contrast to overexpression of the S. cerevisiae Ran/TC4 homologue Gsp1p, previously reported to suppress prp20-1, but not mtr1-2 or srm1-1. Furthermore, gtr1-11 suppressed the rna1-1, temperature-sensitive mutant of the Gsp1p GTPase-activating protein, but not the srp1-31, temperature-sensitive mutant of the S. cerevisiae importin alpha homologue. mtr1-2, srm1-1 and prp20-1 were also suppressed by overexpression of the mutated Gtr1p, Gtr1-11p. In summary, Gtr1p that was localized in the cytoplasm by immunofluoresence staining was suggested to function as a negative regulator for the Ran/TC4 GTPase cycle.

scholarly journals Toward a Comprehensive Temperature-Sensitive Mutant Repository of the Essential Genes of Saccharomyces cerevisiae

2008 ◽  
Vol 30 (2) ◽  
pp. 248-258 ◽  
Author(s):  
Shay Ben-Aroya ◽  
Candice Coombes ◽  
Teresa Kwok ◽  
Kathryn A. O'Donnell ◽  
Jef D. Boeke ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Essential Genes ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant

Characterization of a temperature-sensitive mutant of frog virus 3 defective in DNA replication

Virology ◽  
1981 ◽  
Vol 112 (1) ◽  
pp. 40-48 ◽  
Author(s):  
R. Goorha ◽  
D.B. Willis ◽  
A. Granoff ◽  
R.F. Naegele
Keyword(s):  
Dna Replication ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Frog Virus 3 ◽  
Temperature Sensitive Mutant ◽  
Frog Virus

Temperature Sensitive Mutant of the DNA Replication System in Escherichia coli

Nature ◽  
1970 ◽  
Vol 228 (5277) ◽  
pp. 1157-1160 ◽  
Author(s):  
MASAMICHI KOHIYAMA ◽  
ALAN R. KOLBER
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Replication System

scholarly journals Characterization of a temperature-sensitive mutant of mouse FM3A cells defective in DNA replication.

1985 ◽  
Vol 82 (6) ◽  
pp. 1761-1765 ◽  
Author(s):  
Y. Murakami ◽  
H. Yasuda ◽  
H. Miyazawa ◽  
F. Hanaoka ◽  
M. Yamada
Keyword(s):  
Dna Replication ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Fm3a Cells
Sign in / Sign up

Export Citation Format

Share Document