Molecular mechanisms of action of angiopreventive anti-oxidants on endothelial cells: Microarray gene expression analyses

2005 ◽  
Vol 591 (1-2) ◽  
pp. 198-211 ◽  
Author(s):  
Ulrich Pfeffer ◽  
Nicoletta Ferrari ◽  
Raffaella Dell’Eva ◽  
Stefano Indraccolo ◽  
Monica Morini ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Molecular Mechanisms ◽  
Mechanisms Of Action ◽  
Microarray Gene Expression ◽  
Gene Expression Analyses ◽  
Microarray Gene

Repeatability of published microarray gene expression analyses

2009 ◽  
Vol 41 (2) ◽  
pp. 149-155 ◽  
Author(s):  
John P A Ioannidis ◽  
David B Allison ◽  
Catherine A Ball ◽  
Issa Coulibaly ◽  
Xiangqin Cui ◽  
...  
Keyword(s):  
Gene Expression ◽  
Microarray Gene Expression ◽  
Gene Expression Analyses ◽  
Microarray Gene

scholarly journals Flavanone metabolites decrease monocyte adhesion to TNF-α-activated endothelial cells by modulating expression of atherosclerosis-related genes

2013 ◽  
Vol 110 (4) ◽  
pp. 587-598 ◽  
Author(s):  
Audrey Chanet ◽  
Dragan Milenkovic ◽  
Sylvain Claude ◽  
Jeanette A. M. Maier ◽  
Muhammad Kamran Khan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Vascular Function ◽  
Molecular Mechanisms ◽  
Umbilical Vein ◽  
Inhibitory Effect ◽  
Mechanisms Of Action ◽  
Monocyte Adhesion ◽  
Tnf Α ◽  
The Impact

Flavanones are found specifically and abundantly in citrus fruits. Their beneficial effect on vascular function is well documented. However, little is known about their cellular and molecular mechanisms of action in vascular cells. The goal of the present study was to identify the impact of flavanone metabolites on endothelial cells and decipher the underlying molecular mechanisms of action. We investigated the impact of naringenin and hesperetin metabolites at 0·5, 2 and 10 μm on monocyte adhesion to TNF-α-activated human umbilical vein endothelial cells (HUVEC) and on gene expression. Except hesperetin-7-glucuronide and naringenin-7-glucuronide (N7G), when present at 2 μm, flavanone metabolites (hesperetin-3′-sulphate, hesperetin-3′-glucuronide and naringenin-4′-glucuronide (N4′G)) significantly attenuated monocyte adhesion to TNF-α-activated HUVEC. Exposure of both monocytes and HUVEC to N4′G and N7G at 2 μm resulted in a higher inhibitory effect on monocyte adhesion. Gene expression analysis, using TaqMan Low-Density Array, revealed that flavanone metabolites modulated the expression of genes involved in atherogenesis, such as those involved in inflammation, cell adhesion and cytoskeletal organisation. In conclusion, physiologically relevant concentrations of flavanone metabolites reduce monocyte adhesion to TNF-α-stimulated endothelial cells by affecting the expression of related genes. This provides a potential explanation for the vasculoprotective effects of flavanones.

scholarly journals Consensus guidelines for microarray gene expression analyses in leukemia from three European leukemia networks

Leukemia ◽  
2006 ◽  
Vol 20 (8) ◽  
pp. 1385-1392 ◽  
Author(s):  
F J T Staal ◽  
G Cario ◽  
G Cazzaniga ◽  
T Haferlach ◽  
M Heuser ◽  
...  
Keyword(s):  
Gene Expression ◽  
Consensus Guidelines ◽  
Microarray Gene Expression ◽  
Gene Expression Analyses ◽  
Microarray Gene

Combining Mouse Congenic Strains and Microarray Gene Expression Analyses to Study a Complex Trait: The NOD Model of Type 1 Diabetes

2003 ◽  
Vol 12 (2) ◽  
pp. 232-243 ◽  
Author(s):  
I. A. Eaves ◽  
L. S. Wicker ◽  
G. Ghandour ◽  
P. A. Lyons ◽  
L. B. Peterson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Type 1 Diabetes ◽  
Complex Trait ◽  
Microarray Gene Expression ◽  
Congenic Strains ◽  
Gene Expression Analyses ◽  
Microarray Gene
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