In vitro aging of mineralized collagen-based composite as guided tissue regeneration membrane

2006 ◽  
Vol 26 (4) ◽  
pp. 724-729 ◽  
Author(s):  
S.X. Pan ◽  
Y. Li ◽  
H.L. Feng ◽  
W. Bai ◽  
Y.Y. Gu
Keyword(s):  
Tissue Regeneration ◽  
Guided Tissue Regeneration ◽  
In Vitro Aging ◽  
Mineralized Collagen

FABRICATION AND EVALUATION OF CHITOSAN MEMBRANES FOR GUIDED TISSUE REGENERATION

2004 ◽  
Vol 16 (05) ◽  
pp. 259-264 ◽  
Author(s):  
TA WEI CHEN ◽  
SHYH MING KUO ◽  
SHWU JEN CHANG ◽  
TANG CHING KUAN
Keyword(s):  
Tissue Regeneration ◽  
Guided Tissue Regeneration ◽  
Initial Weight ◽  
Gel Content ◽  
Chitosan Matrix ◽  
Chitosan Membrane ◽  
Chitosan Membranes ◽  
Crosslinking Agents ◽  
Cell Adhesion And Proliferation

Chitosan membranes were prepared by a thermal induced phase separation method, following treatment with nontoxic NaOH gelating and Na5P3O10, Na5SO3 crosslinking agents. Effects of these reaction agents on chitosan membranes were evaluated to survey the feasibility of using these membranes in guided tissue regeneration (GTR) application. The preliminary results showed chitosan membranes crosslinked with Na5P3O10 and Na2SO3 had gel content of 53.5% and 52.2%r, respectively. Contrarily, the chitosan matrix gelated with NaOH dissolved completely during gel content measurement. Chitosan membrane treated with Na5P3O10 had lowest elastic modulus of 12.9 Mpa as compared with other membranes treated with Na2SO3 (17.9Mpa) and NaOH(23.6Mpa). From SEM observations, NaOH gelated chitosan membrane had the smoothest surface morphology than others. However, Na5P3O10 crosslinked chitosan membrane had better cell adhesion and proliferation results in cell culture test. All three chitosan membranes degraded by about 23%∼28% of initial weight after a 90-day in vitro shaking test.

Antimicrobial effect of nanostructured membranes for guided tissue regeneration: an in vitro study

2020 ◽  
Vol 36 (12) ◽  
pp. 1566-1577 ◽  
Author(s):  
J. Bueno ◽  
MC. Sánchez ◽  
M. Toledano-Osorio ◽  
E. Figuero ◽  
M. Toledano ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Guided Tissue Regeneration ◽  
Vitro Study ◽  
Nanostructured Membranes

scholarly journals Preparation and Bioactivity Properties of a Novel Composite Membrane of Fructose Mediatedβ-Tricalcium Pyrophosphate/(Polyethylene Glycol/Chitosan) for Guided Tissue Regeneration

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Jian-Wen Wang ◽  
Min-Hsiung Hon ◽  
Yi-Ming Kuo ◽  
Mei-Hui Chung
Keyword(s):  
Mechanical Property ◽  
Tissue Regeneration ◽  
Composite Membrane ◽  
Degradation Rate ◽  
Composite Membranes ◽  
Guided Tissue Regeneration ◽  
Glycol Chitosan ◽  
Cell Compatibility ◽  
Suitable Material

A novel composite membrane ofβ-tricalcium pyrophosphate (β-TCP) and fructose- (F-) mediated chitosan/poly(ethylene glycol) (CS/PEG) was prepared by thermally induced phase separation technique. The prepared composite membranes were characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD). The mechanical property, swelling, degradation, and cytotoxicity of the composite membranes were evaluated in vitro with respect to its potential for use as biodegradable guided tissue regeneration (GTR) membrane. In vitro degradation tests showed the composite membrane with a controllable degradation rate when changing theβ-TCP content. The incorporation ofβ-TCP granules also caused a significant enhancement of tensile strength. Whenβ-TCP content is controlled to 50 wt%, homogeneous composite membranes with well mechanical property and enzymatic degradation rate can be obtained. Cytotoxicity assay demonstrates that the composite membranes were nontoxic and had very good cell compatibility. Most importantly, the release of calcium ions and glucosamine from the composite membranes was proved to increase the cell proliferation of NIH3T3. The results of this study have indicated that this novel F-β-TCP/CS/PEG composite can be a suitable material for GTR applications.

scholarly journals Development of a membrane for guided tissue regeneration: An in vitro study

2020 ◽  
Vol 31 (5) ◽  
pp. 763
Author(s):  
VanaraF Passos ◽  
RamonC Fernandes ◽  
MárioIgor Damasceno ◽  
Gabriel Pimentel ◽  
JulianoS Mendonça ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
In Vitro Study ◽  
Guided Tissue Regeneration ◽  
Vitro Study

Synthesis, Processing and Testing of a Poly (DL-Lactide-co-ε-Caprolactone) Resorbable Electrospun Membrane for Guided Tissue Regeneration

2012 ◽  
Vol 506 ◽  
pp. 110-113 ◽  
Author(s):  
P. Piyakunakorn ◽  
B. Khumraksa ◽  
B. Thapsukhon ◽  
S. Rassameemasmaung ◽  
Robert Molloy ◽  
...  
Keyword(s):  
Weight Loss ◽  
Tissue Regeneration ◽  
Hydrolytic Degradation ◽  
Guided Tissue Regeneration ◽  
Electrospun Membrane ◽  
3 Dimensional ◽  
Agar Diffusion Test ◽  
The Media ◽  
Phosphate Buffered Saline

The aim of this study was to fabricate 75:25 poly(DL-lactide-co-e-caprolactone), poly(DLL-co-CL) membranes for used in guided tissue regeneration (GTR). The copolymer was synthesized by ring-opening polymerization (ROP) in bulk. The 3-dimensional fiber networks with built-in microporosity membranes were prepared by electrospinning. The pore size was varied between 5-30 μm and the porosity of membrane was 69%. After immersing in phosphate buffered saline (PBS), the membranes were degradable with time, as indicated by molecular weight loss, mass weight loss, reduction of pH of the media and changes in the surface topography and shape of the membranes. However, the in vitro hydrolytic degradation of the membranes was too fast for use as periodontal GTR. The membranes maintained their original shape for the first 4 weeks and the porous structure disappeared within 2 weeks. Results from agar diffusion test suggest the membranes to be non-cytotoxic. In conclusion, the electrospun membrane was non-cytotoxic but the degradation rate was too fast to be used as GTR membrane in periodontal treatment.

scholarly journals In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration

2008 ◽  
Vol 4 (1) ◽  
Author(s):  
Adrian Kasaj ◽  
Christoph Reichert ◽  
Hermann Götz ◽  
Bernd Röhrig ◽  
Ralf Smeets ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Guided Tissue Regeneration ◽  
In Vitro Evaluation ◽  
Barrier Membranes

scholarly journals Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration

2018 ◽  
Vol 5 (3) ◽  
pp. 54 ◽  
Author(s):  
Vishnu Sunandhakumari ◽  
Arun Vidhyadharan ◽  
Aneesh Alim ◽  
Deepan Kumar ◽  
Jayakrishnan Ravindran ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Tissue Regeneration ◽  
Phosphate Buffer Solution ◽  
Composite Membranes ◽  
Guided Tissue Regeneration ◽  
Biologically Active ◽  
Epithelial Tissue ◽  
Periodontal Tissues ◽  
Nanofibrous Membranes

Background: Current resorbable and non-resorbable membranes act as a physical barrier to avoid connective and epithelial tissue downgrowth into the defect, favoring the regeneration of periodontal tissues. These conventional membranes possess many structural and bio-functional limitations. We hypothesized that the next-generation of guided tissue regeneration (GTR) membranes for periodontal tissue engineering will be a biologically active, spatially designed nanofibrous biomaterial that closely mimics the native extra-cellular matrix (ECM). Methods: GTR membranes made of poly(ε-Caprolactone) with a molecular weight of 80,000 reinforced with different weight concentrations of nano-Hydroxyapatite/Bioactive glass (2%, 5%, 10%, 15%) is fabricated by the method of electrospinning. After fabrication, in vitro properties are evaluated. Results: The electrospun nanofibrous membranes possessed excellent mechanical properties initially and after one month of degradation in phosphate buffer solution (PBS). Moreover, none of the fabricated membranes were found to be cytotoxic at lower concentrations and higher concentrations. Comparing the overall properties, PCL (poly(e-caprolactone)) + BG (Bioactive glass) 2% exhibited superior cell attachment and percentage of viable cells, increased fiber and pore diameter which satisfies the ideal properties needed for GTR membranes. Conclusion: Composite nanofibrous membranes prepared by electrospinning are suitable for use as a GTR membrane and are a useful prototype for further development of a final membrane for clinical use.

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