FABRICATION AND EVALUATION OF CHITOSAN MEMBRANES FOR GUIDED TISSUE REGENERATION

2004 ◽  
Vol 16 (05) ◽  
pp. 259-264 ◽  
Author(s):  
TA WEI CHEN ◽  
SHYH MING KUO ◽  
SHWU JEN CHANG ◽  
TANG CHING KUAN
Keyword(s):  
Tissue Regeneration ◽  
Guided Tissue Regeneration ◽  
Initial Weight ◽  
Gel Content ◽  
Chitosan Matrix ◽  
Chitosan Membrane ◽  
Chitosan Membranes ◽  
Crosslinking Agents ◽  
Cell Adhesion And Proliferation

Chitosan membranes were prepared by a thermal induced phase separation method, following treatment with nontoxic NaOH gelating and Na5P3O10, Na5SO3 crosslinking agents. Effects of these reaction agents on chitosan membranes were evaluated to survey the feasibility of using these membranes in guided tissue regeneration (GTR) application. The preliminary results showed chitosan membranes crosslinked with Na5P3O10 and Na2SO3 had gel content of 53.5% and 52.2%r, respectively. Contrarily, the chitosan matrix gelated with NaOH dissolved completely during gel content measurement. Chitosan membrane treated with Na5P3O10 had lowest elastic modulus of 12.9 Mpa as compared with other membranes treated with Na2SO3 (17.9Mpa) and NaOH(23.6Mpa). From SEM observations, NaOH gelated chitosan membrane had the smoothest surface morphology than others. However, Na5P3O10 crosslinked chitosan membrane had better cell adhesion and proliferation results in cell culture test. All three chitosan membranes degraded by about 23%∼28% of initial weight after a 90-day in vitro shaking test.

Hybrid Chitosan Membranes Tested in Sheep for Guided Tissue Regeneration

2007 ◽  
pp. 1265-1268
Author(s):  
P.P. Cortez ◽  
Yuki Shirosaki ◽  
C.M. Botelho ◽  
M.J. Simões ◽  
F. Gartner ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Guided Tissue Regeneration ◽  
Chitosan Membranes

Guided tissue regeneration for using a chitosan membrane: An experimental study in rats

2005 ◽  
Vol 76A (2) ◽  
pp. 408-415 ◽  
Author(s):  
Shyh Ming Kuo ◽  
Shwu Jen Chang ◽  
Ta Wei Chen ◽  
Tang Ching Kuan
Keyword(s):  
Experimental Study ◽  
Tissue Regeneration ◽  
Guided Tissue Regeneration ◽  
Chitosan Membrane

Hybrid Chitosan Membranes Tested in Sheep for Guided Tissue Regeneration

2007 ◽  
Vol 361-363 ◽  
pp. 1265-1268 ◽  
Author(s):  
P.P. Cortez ◽  
Yuki Shirosaki ◽  
C.M. Botelho ◽  
M.J. Simões ◽  
F. Gartner ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Tissue Regeneration ◽  
Chitosan Solution ◽  
Clinical Applications ◽  
Histological Evaluation ◽  
Sheep Model ◽  
The Third ◽  
Chitosan Membranes

Previous in vitro studies confirmed an improved cytocompatibility of chitosan-silicate hybrid membranes over chitosan membranes. The main goal of this study was to assess the in vivo histocompatibility of both membranes through subcutaneous implantations at different time periods, 1 week, 1, 2 and 3 months, using a sheep model. Chitosan membranes elicited an exuberant inflammatory response and were consequently rejected. The hybrid chitosan membranes were not rejected and the degree of inflammatory response decreased gradually until the third month of implantation. Histological evaluation also showed that these membranes can be resorbed in vivo. This study demonstrates that the incorporation of silicate into the chitosan solution improves its histocompatibility, indicating that the hybrid chitosan-silicate membranes are suitable candidates to be used in clinical applications.

Antimicrobial effect of nanostructured membranes for guided tissue regeneration: an in vitro study

2020 ◽  
Vol 36 (12) ◽  
pp. 1566-1577 ◽  
Author(s):  
J. Bueno ◽  
MC. Sánchez ◽  
M. Toledano-Osorio ◽  
E. Figuero ◽  
M. Toledano ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Guided Tissue Regeneration ◽  
Vitro Study ◽  
Nanostructured Membranes

scholarly journals Preparation and Bioactivity Properties of a Novel Composite Membrane of Fructose Mediatedβ-Tricalcium Pyrophosphate/(Polyethylene Glycol/Chitosan) for Guided Tissue Regeneration

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Jian-Wen Wang ◽  
Min-Hsiung Hon ◽  
Yi-Ming Kuo ◽  
Mei-Hui Chung
Keyword(s):  
Mechanical Property ◽  
Tissue Regeneration ◽  
Composite Membrane ◽  
Degradation Rate ◽  
Composite Membranes ◽  
Guided Tissue Regeneration ◽  
Glycol Chitosan ◽  
Cell Compatibility ◽  
Suitable Material

A novel composite membrane ofβ-tricalcium pyrophosphate (β-TCP) and fructose- (F-) mediated chitosan/poly(ethylene glycol) (CS/PEG) was prepared by thermally induced phase separation technique. The prepared composite membranes were characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD). The mechanical property, swelling, degradation, and cytotoxicity of the composite membranes were evaluated in vitro with respect to its potential for use as biodegradable guided tissue regeneration (GTR) membrane. In vitro degradation tests showed the composite membrane with a controllable degradation rate when changing theβ-TCP content. The incorporation ofβ-TCP granules also caused a significant enhancement of tensile strength. Whenβ-TCP content is controlled to 50 wt%, homogeneous composite membranes with well mechanical property and enzymatic degradation rate can be obtained. Cytotoxicity assay demonstrates that the composite membranes were nontoxic and had very good cell compatibility. Most importantly, the release of calcium ions and glucosamine from the composite membranes was proved to increase the cell proliferation of NIH3T3. The results of this study have indicated that this novel F-β-TCP/CS/PEG composite can be a suitable material for GTR applications.

scholarly journals Development of a membrane for guided tissue regeneration: An in vitro study

2020 ◽  
Vol 31 (5) ◽  
pp. 763
Author(s):  
VanaraF Passos ◽  
RamonC Fernandes ◽  
MárioIgor Damasceno ◽  
Gabriel Pimentel ◽  
JulianoS Mendonça ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
In Vitro Study ◽  
Guided Tissue Regeneration ◽  
Vitro Study
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