Preparation and Bioactivity Properties of a Novel Composite Membrane of Fructose Mediatedβ-Tricalcium Pyrophosphate/(Polyethylene Glycol/Chitosan) for Guided Tissue Regeneration

A novel composite membrane ofβ-tricalcium pyrophosphate (β-TCP) and fructose- (F-) mediated chitosan/poly(ethylene glycol) (CS/PEG) was prepared by thermally induced phase separation technique. The prepared composite membranes were characterized using scanning electron microscopy (SEM) and X-ray diffraction (XRD). The mechanical property, swelling, degradation, and cytotoxicity of the composite membranes were evaluated in vitro with respect to its potential for use as biodegradable guided tissue regeneration (GTR) membrane. In vitro degradation tests showed the composite membrane with a controllable degradation rate when changing theβ-TCP content. The incorporation ofβ-TCP granules also caused a significant enhancement of tensile strength. Whenβ-TCP content is controlled to 50 wt%, homogeneous composite membranes with well mechanical property and enzymatic degradation rate can be obtained. Cytotoxicity assay demonstrates that the composite membranes were nontoxic and had very good cell compatibility. Most importantly, the release of calcium ions and glucosamine from the composite membranes was proved to increase the cell proliferation of NIH3T3. The results of this study have indicated that this novel F-β-TCP/CS/PEG composite can be a suitable material for GTR applications.

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Fabrication and In Vitro Characterization of Bioactive Glass/Nano Hydroxyapatite Reinforced Electrospun Poly(ε-Caprolactone) Composite Membranes for Guided Tissue Regeneration

Bioengineering ◽

10.3390/bioengineering5030054 ◽

2018 ◽

Vol 5 (3) ◽

pp. 54 ◽

Cited By ~ 4

Author(s):

Vishnu Sunandhakumari ◽

Arun Vidhyadharan ◽

Aneesh Alim ◽

Deepan Kumar ◽

Jayakrishnan Ravindran ◽

...

Keyword(s):

Bioactive Glass ◽

Tissue Regeneration ◽

Phosphate Buffer Solution ◽

Composite Membranes ◽

Guided Tissue Regeneration ◽

Biologically Active ◽

Epithelial Tissue ◽

Periodontal Tissues ◽

Nanofibrous Membranes

Background: Current resorbable and non-resorbable membranes act as a physical barrier to avoid connective and epithelial tissue downgrowth into the defect, favoring the regeneration of periodontal tissues. These conventional membranes possess many structural and bio-functional limitations. We hypothesized that the next-generation of guided tissue regeneration (GTR) membranes for periodontal tissue engineering will be a biologically active, spatially designed nanofibrous biomaterial that closely mimics the native extra-cellular matrix (ECM). Methods: GTR membranes made of poly(ε-Caprolactone) with a molecular weight of 80,000 reinforced with different weight concentrations of nano-Hydroxyapatite/Bioactive glass (2%, 5%, 10%, 15%) is fabricated by the method of electrospinning. After fabrication, in vitro properties are evaluated. Results: The electrospun nanofibrous membranes possessed excellent mechanical properties initially and after one month of degradation in phosphate buffer solution (PBS). Moreover, none of the fabricated membranes were found to be cytotoxic at lower concentrations and higher concentrations. Comparing the overall properties, PCL (poly(e-caprolactone)) + BG (Bioactive glass) 2% exhibited superior cell attachment and percentage of viable cells, increased fiber and pore diameter which satisfies the ideal properties needed for GTR membranes. Conclusion: Composite nanofibrous membranes prepared by electrospinning are suitable for use as a GTR membrane and are a useful prototype for further development of a final membrane for clinical use.

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PD043: In vitro evaluation of graft material displacement with different membranes following guided tissue regeneration on grade ii furcation defects

Journal Of Clinical Periodontology ◽

10.1111/jcpe.44_12914 ◽

2018 ◽

Vol 45 ◽

pp. 56-56

Keyword(s):

Tissue Regeneration ◽

Guided Tissue Regeneration ◽

Graft Material ◽

In Vitro Evaluation ◽

Furcation Defects ◽

Grade Ii

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Mechanical and In-Vitro Cell Compatibility Properties of Silk-Elastinlike Protein-Based Biomaterial

ASME 2010 First Global Congress on NanoEngineering for Medicine and Biology ◽

10.1115/nemb2010-13141 ◽

2010 ◽

Author(s):

Weibing Teng ◽

Yiding Huang ◽

Joseph Cappello ◽

Xiaoyi Wu

Keyword(s):

Mechanical Property ◽

Cell Culture ◽

Mechanical Strength ◽

Genetically Engineered ◽

Load Bearing ◽

Cell Compatibility ◽

Chemical Treatments ◽

Good Biocompatibility ◽

Physical And Chemical

A series of genetically engineered recombinant silk-elastinlike proteins (SELPs) have been produced by combining polypeptide sequences derived from native silk of superior mechanical strength and elastin that is extremely durable and resilient. They have displayed a set of outstanding properties such as good biocompatibility and controllable biodegradation rates. In the study, we characterized the mechanical property of genetically engineered, recombinant silk-elastinlike protein copolymer, SELP-47K, under physical and chemical treatments. The biocompatibility of the SELP-47K was also evaluated by cell culture. The ultimate goal of this study is to explore the potential of SELPs for applications in the engineering of load-bearing tissues such as arteries.

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FABRICATION AND EVALUATION OF CHITOSAN MEMBRANES FOR GUIDED TISSUE REGENERATION

Biomedical Engineering Applications Basis and Communications ◽

10.4015/s1016237204000360 ◽

2004 ◽

Vol 16 (05) ◽

pp. 259-264 ◽

Cited By ~ 5

Author(s):

TA WEI CHEN ◽

SHYH MING KUO ◽

SHWU JEN CHANG ◽

TANG CHING KUAN

Keyword(s):

Tissue Regeneration ◽

Guided Tissue Regeneration ◽

Initial Weight ◽

Gel Content ◽

Chitosan Matrix ◽

Chitosan Membrane ◽

Chitosan Membranes ◽

Crosslinking Agents ◽

Cell Adhesion And Proliferation

Chitosan membranes were prepared by a thermal induced phase separation method, following treatment with nontoxic NaOH gelating and Na5P3O10, Na5SO3 crosslinking agents. Effects of these reaction agents on chitosan membranes were evaluated to survey the feasibility of using these membranes in guided tissue regeneration (GTR) application. The preliminary results showed chitosan membranes crosslinked with Na5P3O10 and Na2SO3 had gel content of 53.5% and 52.2%r, respectively. Contrarily, the chitosan matrix gelated with NaOH dissolved completely during gel content measurement. Chitosan membrane treated with Na5P3O10 had lowest elastic modulus of 12.9 Mpa as compared with other membranes treated with Na2SO3 (17.9Mpa) and NaOH(23.6Mpa). From SEM observations, NaOH gelated chitosan membrane had the smoothest surface morphology than others. However, Na5P3O10 crosslinked chitosan membrane had better cell adhesion and proliferation results in cell culture test. All three chitosan membranes degraded by about 23%∼28% of initial weight after a 90-day in vitro shaking test.

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Enhanced Critical Size Defect Repair in Rabbit Mandible by Electrospun Gelatin/β-TCP Composite Nanofibrous Membranes

Journal of Nanomaterials ◽

10.1155/2015/396916 ◽

2015 ◽

Vol 2015 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Mingming Xu ◽

Xuehui Zhang ◽

Song Meng ◽

Xiaohan Dai ◽

Bing Han ◽

...

Keyword(s):

Tissue Regeneration ◽

Bone Repair ◽

Immunohistochemical Analysis ◽

Composite Membranes ◽

Nanofibrous Membrane ◽

Porous Network ◽

Barrier Membranes ◽

Biological Performance ◽

Electrospun Gelatin

The design and fabrication of biodegradable barrier membranes with satisfactory structure and composition remain a considerable challenge for periodontal tissue regeneration. We have developed a biomimetic nanofibrous membrane made from a composite of gelatin andβ-tricalcium phosphate (β-TCP). We previously confirmed thein vitrobiological performance of the membrane material, but the efficacy of the membranes in promoting bone repairin situhas not yet been examined. Gelatin/β-TCP composite nanofibers were fabricated by incorporation of 20 wt.%β-TCP nanoparticles into electrospun gelatin nanofibers. Electron microscopy showed that the composite membranes presented a nonwoven structure with an interconnected porous network and had a rough surface due to theβ-TCP nanoparticles, which were distributed widely and uniformly throughout the gelatin-fiber matrix. The repair efficacy of rabbit mandible defects implanted with bone substitute (Bio-Oss) and covered with the gelatin/β-TCP composite nanofibrous membrane was evaluated in comparison with pure gelatin nanofibrous membrane. Gross observation, histological examination, and immunohistochemical analysis showed that new bone formation and defect closure were significantly enhanced by the composite membranes compared to the pure gelatin ones. From these results, we conclude that nanofibrous gelatin/β-TCP composite membranes could serve as effective barrier membranes for guided tissue regeneration.

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Bioactivity and biocompatibility of a chitosan-tobermorite composite membrane for guided tissue regeneration

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2013.11.020 ◽

2014 ◽

Vol 64 ◽

pp. 11-16 ◽

Cited By ~ 30

Author(s):

A.P. Hurt ◽

G. Getti ◽

N.J. Coleman

Keyword(s):

Tissue Regeneration ◽

Composite Membrane ◽

Guided Tissue Regeneration

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Factorial Study of Compressive Mechanical Properties and PrimaryIn VitroOsteoblast Response of PHBV/PLLA Scaffolds

Journal of Nanomaterials ◽

10.1155/2012/656914 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 4

Author(s):

Naznin Sultana ◽

Tareef Hayat Khan

Keyword(s):

Mechanical Properties ◽

Polymer Matrix ◽

Tissue Regeneration ◽

Degradation Rate ◽

Bone Tissue Regeneration ◽

Osteoblastic Cells ◽

Composite Scaffolds ◽

Biomimetic Systems ◽

Human Osteoblastic Cells

For bone tissue regeneration, composite scaffolds containing biodegradable polymers and nanosized osteoconductive bioceramics have been regarded as promising biomimetic systems. Polymer blends of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and poly(L-lactic acid) (PLLA) can be used as the polymer matrix to control the degradation rate. In order to render the scaffolds osteoconductive, nano-sized hydroxyapatite (nHA) particles can be incorporated into the polymer matrix. In the first part of this study, a factorial design approach to investigate the influence of materials on the initial compressive mechanical properties of the scaffolds was studied. In the second part, the protein adsorption behavior and the attachment and morphology of osteoblast-like cells (Saos-2) of the scaffoldsin vitrowere also studied. It was observed that nHA incorporated PHBV/PLLA composite scaffolds adsorbed more bovine serum albumin (BSA) protein than PHBV or PHBV/PLLA scaffolds.In vitrostudies also revealed that the attachment of human osteoblastic cells (SaOS-2) was significantly higher in nHA incorporated PHBV/PLLA composite scaffolds. From the SEM micrographs of nHA incorporated PHBV/PLLA composite scaffolds seeded with SaOS-2 cells after a 7-day cell culture period, it was observed that the cells were well expanded and spread in all directions on the scaffolds.

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