Rapid gelation of oxidized hyaluronic acid and succinyl chitosan for integration with insulin-loaded micelles and epidermal growth factor on diabetic wound healing

Diabetic wound, a common complication in patients with diabetes, is difficult to treat because of the long-term consequences of accumulating advanced glycation end products and high glucose levels. Moreover, wound infections occur frequently in diabetes; therefore, developing effective treatments is of great significance for healing diabetic wounds. Electrospun nanofibers have played a favorable role in wound healing, but the efficacy in diabetes is yet to be investigated. This study used mouse models of DW in order to determine the effect of electrospun nanofibers on wound healing. It turned out that electrospun nanofibers increased the healing rate in mice and inhibited levels of inflammatory cytokines, as well as activated epidermal growth factor (EGF). In vitro experiments revealed that the migration and proliferation of human umbilical vein endothelial cells (HUVECs) were enhanced after the intervention. Overall, electrospun nanofibers can improve the healing of diabetic wounds significantly.

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04 / Epidermal growth factor administration to promote oral wound healing: a preclinical study

10.26226/morressier.5ac383202afeeb00097a3cc6 ◽

2018 ◽

Author(s):

Heithem Ben Amara

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Preclinical Study ◽

Epidermal Growth ◽

Oral Wound Healing

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Development of stabilized dual growth factor-loaded hyaluronate collagen dressing matrix

Journal of Tissue Engineering ◽

10.1177/2041731421999750 ◽

2021 ◽

Vol 12 ◽

pp. 204173142199975

Author(s):

Jihyun Kim ◽

Kyoung-Mi Lee ◽

Seung Hwan Han ◽

Eun Ae Ko ◽

Dong Suk Yoon ◽

...

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Type I ◽

Diabetic Mice ◽

Patients With Diabetes ◽

Diabetic Wound Healing ◽

Epidermal Growth ◽

Wound Healing Effect ◽

Growth Factor Production

Patients with diabetes experience impaired growth factor production such as epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and they are reportedly involved in wound healing processes. Here, we report dual growth factor-loaded hyaluronate collagen dressing (Dual-HCD) matrix, using different ratios of the concentration of stabilized growth factors—stabilized-EGF (S-EGF) and stabilized-bFGF (S-bFGF). At first, the optimal concentration ratio of S-EGF to S-bFGF in the Dual-HCD matrix is determined to be 1:2 in type I diabetic mice. This Dual-HCD matrix does not cause cytotoxicity and can be used in vivo. The wound-healing effect of this matrix is confirmed in type II diabetic mice. Dual HCD enhances angiogenesis which promotes wound healing and thus, it shows a significantly greater synergistic effect than the HCD matrix loaded with a single growth factor. Overall, we conclude that the Dual-HCD matrix represents an effective therapeutic agent for impaired diabetic wound healing.

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