Psoriatic mesenchymal stem cells stimulate the angiogenesis of human umbilical vein endothelial cells in vitro

Bone is an active tissue where bone mineralization and resorption occur simultaneously. In the case of fracture, there are numerous factors required to facilitate bone healing including precursor cells and blood vessels. To evaluate the interaction between bone marrow-derived mesenchymal stem cells (BMSC)—the precursor cells able to differentiate into bone-forming cells and human umbilical vein endothelial cells (HUVEC)—a cell source widely used for the study of blood vessels. We performed direct coculture of BMSC and HUVEC in normoxia and chemically induced hypoxia using Cobalt(II) chloride and Dimethyloxaloylglycine and in the condition where oxygen level was maintained at 1% as well. Cell proliferation was analyzed by crystal violet staining. Osteogenesis was examined by Alizarin Red and Collagen type I staining. Expression of angiogenic factor-vascular endothelial growth factor (VEGF) and endothelial marker-von Willebrand factor (VWF) were demonstrated by immunohistochemistry and enzyme-linked immunosorbent assay. The quantitative polymerase chain reaction was also used to evaluate gene expression. The results showed that coculture in normoxia could retain both osteogenic differentiation and endothelial markers while hypoxic condition limits cell proliferation and osteogenesis but favors the angiogenic function even after 1 of day treatment.

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Co-culture of mesenchymal stem cells and human umbilical vein endothelial cells on heparinized polycaprolactone/gelatin co-spun nanofibers for improved endothelium remodeling

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2020.02.163 ◽

2020 ◽

Vol 151 ◽

pp. 186-192 ◽

Cited By ~ 1

Author(s):

Akshat Joshi ◽

Zhe Xu ◽

Yasuhiro Ikegami ◽

Soichiro Yamane ◽

Masanori Tsurashima ◽

...

Keyword(s):

Stem Cells ◽

Endothelial Cells ◽

Mesenchymal Stem Cells ◽

Umbilical Vein ◽

Human Umbilical Vein ◽

Umbilical Vein Endothelial Cells

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Effects of leptin-modified human placenta-derived mesenchymal stem cells on angiogenic potential and peripheral inflammation of human umbilical vein endothelial cells (HUVECs) after X-ray radiation

Journal of Zhejiang University SCIENCE B ◽

10.1631/jzus.b1900598 ◽

2020 ◽

Vol 21 (4) ◽

pp. 327-340

Author(s):

Shu Chen ◽

Qian Wang ◽

Bing Han ◽

Jia Wu ◽

Ding-kun Liu ◽

...

Keyword(s):

Stem Cells ◽

Endothelial Cells ◽

Mesenchymal Stem Cells ◽

Human Placenta ◽

Umbilical Vein ◽

Peripheral Inflammation ◽

Human Umbilical Vein ◽

X Ray ◽

Angiogenic Potential ◽

Umbilical Vein Endothelial Cells

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The synergistic effect on osteogenic differentiation of human mesenchymal stem cells by diode laser-treated stimulating human umbilical vein endothelial cells

Laser Physics Letters ◽

10.1088/1612-2011/13/2/025604 ◽

2015 ◽

Vol 13 (2) ◽

pp. 025604 ◽

Cited By ~ 4

Author(s):

Chia-Tze Kao ◽

Tuan-Ti Hsu ◽

Tsui-Hsien Huang ◽

Yu-Tin Wu ◽

Yi-Wen Chen ◽

...

Keyword(s):

Stem Cells ◽

Endothelial Cells ◽

Mesenchymal Stem Cells ◽

Synergistic Effect ◽

Osteogenic Differentiation ◽

Diode Laser ◽

Umbilical Vein ◽

Human Mesenchymal Stem Cells ◽

Human Umbilical Vein ◽

Umbilical Vein Endothelial Cells

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The CNGRCLLII(KLAKLAK)2 peptide shows cytotoxicity against HUVECs by inducing apoptosis: An in vitro and in vivo study

Tumor Biology ◽

10.1177/1010428317701649 ◽

2017 ◽

Vol 39 (5) ◽

pp. 101042831770164

Author(s):

Guanying Wang ◽

Na Yuan ◽

Shangke Huang ◽

Lu Feng ◽

Rui Han ◽

...

Keyword(s):

Stem Cells ◽

Endothelial Cells ◽

Vascular Endothelial Cells ◽

Umbilical Vein ◽

Adipose Derived Stem Cells ◽

Human Umbilical Vein ◽

Tumor Tissues ◽

Umbilical Vein Endothelial Cells

Fibrinogen Asn-Gly-Arg motif can specifically recognize and bind to Aminopeptidase N (CD13) on vascular endothelial cells in newly formed tumor vessels. Adipose-derived stem cells can serve as ideal vectors for gene therapy because of their ability of migrating to tumor tissues. First, this study was aimed to design a new peptide (CNGRCLLII(KLAKLAK)2) named CNAK which contains cyclic Asn-Gly-Arg motif and test its biological activity against human umbilical vein endothelial cells. Second, we aimed to construct stably transfected adipose-derived stem cells which express the CNAK peptide and investigate their anti-angiogenic activity in vivo. Adipose-derived stem cells were employed to localize CNAK on vascular endothelial cells in tumors based on their homing property. First of all, the new peptide was synthesized, which effectively entered into CD13+ human umbilical vein endothelial cells and showed cytotoxicity against human umbilical vein endothelial cells. The peptide induced apoptosis of human umbilical vein endothelial cells in a time- and dose-dependent manner, inhibited the expression of Bcl-2, and promoted the expression of Caspase-3 in human umbilical vein endothelial cells. Furthermore, the migration and tube formation of human umbilical vein endothelial cells were inhibited by CNAK. Primary adipose-derived stem cells were then isolated and identified. Stably transfected adipose-derived stem cells which express CNAK peptide (CNAK-ASCs) were successfully established, and the migration of CNAK-ASCs was assessed. In vivo, CNAK-ASCs were found to inhibit the growth and angiogenesis of breast cancer xenografts. This effect may be through inhibiting the secretion of matrix metalloproteinase-2 and membrane type 1-matrix metalloproteinase in vivo. It was also found that CNAK-ASCs reduced the quantity of breast cancer stem cells in tumor tissues. Our data suggested that the new peptide CNAK containing Asn-Gly-Arg motif had anti-angiogenic activity in vitro and in vivo.

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