Effects of initial medium pH and temperature on growth and citric acid production kinetics of a novel domestic Yarrowia lipolytica strain

2012 ◽  
Vol 29 ◽  
pp. S62 ◽  
Author(s):  
Seda Karasu Yalcin ◽  
M. Tijen Bozdemir ◽  
Z. Yesim Ozbas
2013 ◽  
Vol 3 (8) ◽  
Author(s):  
Luana V. da Silva ◽  
Camila B. Tavares ◽  
Roberta dos R. Ribeiro ◽  
Fernando L. P. Pessoa ◽  
Maria Alice Z. Coelho ◽  
...  

2020 ◽  
Vol 10 (20) ◽  
pp. 7373
Author(s):  
Seraphim Papanikolaou ◽  
Panagiota Diamantopoulou ◽  
Fabrice Blanchard ◽  
Eleni Lambrinea ◽  
Isabelle Chevalot ◽  
...  

A new yeast wild-type Yarrowia lipolytica isolate presented efficient growth on glycerol. During flask cultures, nitrogen limitation led to the secretion of sugar-alcohols as the major metabolites of the process (mannitol, arabitol and erythritol), whereas insignificant quantities of citrate were synthesized. Although in some instances high initial glycerol concentrations were employed (≈150 g/L), remarkable glycerol assimilation and polyol secretion was observed. Total polyols ≈ 52 g/L (conversion yield on glycerol consumed = 0.43 g/g) was recorded in the flask experiments. The sugar-alcohol production bioprocess was successfully simulated with the aid of a modified Velhlust–Aggelis model that fitted very well with the experimental data, while optimized parameter values seemed to be quite consistent. In bioreactor trials, a noticeable metabolic shift towards citric acid production was observed, while simultaneously insignificant polyol quantities were produced. In fed-batch bioreactor experiments, a total citric acid quantity ≈ 102 g/L was recorded—one of the highest in the literature for wild-type Y. lipolytica strains. This metabolic transition was due to higher oxygen saturation into the medium that occurred in the bioreactor experiments compared with the flasks. Cellular lipids produced in the bioreactor trial contained higher concentrations of unsaturated fatty acids compared with those produced in flasks.


2009 ◽  
Vol 26 (7) ◽  
pp. 1217-1224 ◽  
Author(s):  
Anita Rywińska ◽  
Waldemar Rymowicz ◽  
Barbara Żarowska ◽  
Adam Skrzypiński

2010 ◽  
Vol 12 (5) ◽  
pp. 469-476 ◽  
Author(s):  
Xiao-Yan Liu ◽  
Zhe Chi ◽  
Guang-Lei Liu ◽  
Fang Wang ◽  
Catherine Madzak ◽  
...  

2007 ◽  
Vol 77 (4) ◽  
pp. 861-869 ◽  
Author(s):  
André Förster ◽  
Kordula Jacobs ◽  
Thomas Juretzek ◽  
Stephan Mauersberger ◽  
Gerold Barth

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