Optimization of production conditions for Trichoderma sp. P7 as a biocontrol agent in liquid culture

The production of microsclerotia by Purpureocillium lilacinum in liquid culture for use as a biocontrol agent for management of root-knot nematode, Meloidogyne incognita, has not been described. To investigate the potential for microsclerotia production, P. lilacinum strain CQPL01 was cultured in liquid media containing various concentrations of ferrous sulphate. Under these conditions mycelia began to form microsclerotia. The maximum yield (11.8 × 104 microsclerotia ml−1) was obtained in medium containing 0.2 g l−1 ferrous sulphate and the greatest production of conidia (1.3 × 108 conidia g−1) was obtained by the culture of air-dried microsclerotia in aqueous agar medium. Subsequently, the viability of microsclerotia, including stress resistance, storage stability and pathogenicity against M. incognita, was investigated. The microsclerotia exhibited excellent nematophagous ability and greater thermotolerance and UV-B radiation tolerance compared to conidia. These results suggested that microsclerotia propagules might be superior to the use of conidia in P. lilacinum biocontrol products.

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On visions and promises — ethical aspects of in vitro meat

Emerging Topics in Life Sciences ◽

10.1042/etls20190108 ◽

2019 ◽

Vol 3 (6) ◽

pp. 753-758

Author(s):

Silvia Woll

Keyword(s):

Animal Welfare ◽

Environmental Issues ◽

Meat Production ◽

Ethical Aspects ◽

Existing Problems ◽

In Vitro Meat ◽

Production And Consumption ◽

Production Conditions

Innovators of in vitro meat (IVM) are convinced that this approach is the solution for problems related to current meat production and consumption, especially regarding animal welfare and environmental issues. However, the production conditions have yet to be fully clarified and there is still a lack of ethical discourses and critical debates on IVM. In consequence, discussion about the ethical justifiability and desirability of IVM remains hypothetical and we have to question those promises. This paper addresses the complex ethical aspects associated with IVM and the questions of whether, and under what conditions, the production of IVM represents an ethically justifiable solution for existing problems, especially in view of animal welfare, the environment, and society. There are particular hopes regarding the benefits that IVM could bring to animal welfare and the environment, but there are also strong doubts about their ethical benefits.

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Insecticidal bait development for control of Musca Domestica L.

Veterinaria Kubani ◽

10.33861/2071-8020-2020-6-28-30 ◽

2020 ◽

pp. 28-30

Author(s):

Mikhail A. Levchenko ◽

Keyword(s):

Musca Domestica ◽

Poultry Farm ◽

Laboratory Studies ◽

Scientific Center ◽

Insecticidal Effect ◽

Invasive Diseases ◽

Previous Level ◽

Group Feeding ◽

Livestock Building ◽

Production Conditions

The control of houseflies (Musca domestica L.) in veterinary surveillance premises is an important measure for the welfare of animals against infectious and invasive diseases. For this purpose, the most effective chemical insecticides are used. To prevent possible resistance to them, bait insecticides with two active binary ingredients from different chemical classes are used. The work was carried out in the laboratory of the Tyumen Scientific Center and in the production conditions of ZAO Pyshminskaya Poultry Farm. This material presents the main stages in the development of a prototype of the insecticidal bait Mukhnet AX containing two insecticides: 1.5% acetamiprid and 6% chlorfenapir. For this purpose, adults of houseflies Musca domestica L. 3-5 days old were used. Under laboratory conditions, effective rational doses of the above insecticides were determined by group feeding, feeding and by the method of assessing food insecticidal baits when fighting flies from 0.00002 to 4% concentrations. According to the results of laboratory studies, it was found that the optimal doses causing 100% death of insects were 0.5% for acetamiprid and 2% for chlorfenapir. The insecticidal efficiency of the developed bait Mukhnet AH against flies in production conditions on the first day after the treatment of the livestock building was 91.25%. The insecticidal effect of the measures taken lasted for at least 6 days. The restoration of the number to the previous level of the number of insects occurred after 10 days. Based on the results obtained, the Method of using the composition of an insecticidal bait agent in the fight against Musca domestica was proposed and patented.

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Biology of the Introduced Biocontrol Agent Microctonus hyperodae (Hymenoptera: Braconidae) and Its Host Listronotus bonariensis (Coleoptera: Curculionidae) in Northern New Zealand

Environmental Entomology ◽

10.1603/en11246 ◽

2013 ◽

Keyword(s):

New Zealand ◽

Biocontrol Agent ◽

Listronotus Bonariensis ◽

Microctonus Hyperodae

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