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Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 66
Author(s):  
Marta Wójcik ◽  
Klaudia Stachal ◽  
Mateusz Burzec ◽  
Kamil Gruszczyński ◽  
Agnieszka Korga-Plewko

Our goal was to determine the responses of body weight (BW) and bone hydroxyproline (Hyp) concentration in turkeys fed a corn silage (CS) diet with different values of dietary cation–anion differences (DCADs). The turkeys (n = 90) were divided into five groups and fed as follows: group A (control)—standard diet (SD) (60%) plus CS (40%); group B—SD (60%), CS (40%) plus 240 g of CaCl2 per 100 kg of diet; group C—SD (60%), CS (40%) plus 480 g of CaCl2 per 100 kg of diet; group D—SD (60%), CS (40%) plus 240 g of NaHCO3 per 100 kg of diet; group E—SD (60%), CS (40%) plus 480 g NaHCO3 per 100 kg of diet. The addition of a lesser amount of CaCl2 lowered the DCAD, which ranged between 52.5 ± 4.19 and 91.14 ± 3.14 mEq/kg DM. An increased content of CaCl2 led to high negative values of DCAD. NaHCO3 supplemented in both doses resulted in a significant elevation of DCAD. Compared to each experimental group, feeding birds with a diet supplemented only with CS resulted in a lower BW. Addition of CaCl2 or NaHCO3 to the diet improved BW, but only CaCl2 addition enhanced the bone Hyp amount. In conclusion, we suggest that an anionic diet with low DCAD can prevent bone abnormalities in large turkeys, especially in the final course of production.


2021 ◽  
Vol 1 ◽  
Author(s):  
Verena Christen ◽  
Daniela Grossar ◽  
Jean-Daniel Charrière ◽  
Michael Eyer ◽  
Lukas Jeker

Neonicotinoids as thiamethoxam and thiacloprid are suspected to be implicated in the decline of honey bee populations. As nicotinic acetylcholine receptor agonists, they disturb acetylcholine receptor signaling in insects, leading to neurotoxicity and are therefore globally used as insecticides. Several behavioral studies have shown links between neonicotinoid exposure of bees and adverse effects on foraging activity, homing flight performance and reproduction, but the molecular aspects underlying these effects are not well-understood. In the last years, several studies through us and others showed the effects of exposure to neonicotinoids on gene expression in the brain of honey bees. Transcripts of acetylcholine receptors, hormonal regulation, stress markers, detoxification enzymes, immune system related genes and transcripts of the energy metabolism were altered after neonicotinoid exposure. To elucidate the link between homing flight performance and shifts in gene expression in the brain of honey bees after neonicotinoid exposure, we combined homing flight activity experiments applying RFID technology and gene expression analysis. We analyzed the expression of endocrine factors, stress genes, detoxification enzymes and genes linked to energy metabolism in forager bees after homing flight experiments. Three different experiments (experiment I: pilot study; experiment II: “worst-case” study and experiment III: laboratory study) were performed. In a pilot study, we wanted to investigate if we could see differences in gene expression between controls and exposed bees (experiment I). This first study was followed by a so-called “worst-case” study (experiment II), where we investigated mainly differences in the expression of transcripts linked to energy metabolism between fast and slow returning foragers. We found a correlation between homing flight duration and the expression of cytochrome c oxidase subunit 5A, one transcript linked to oxidative phosphorylation. In the third experiment (experiment III), foragers were exposed in the laboratory to 1 ng/bee thiamethoxam and 8 ng/bee thiacloprid followed by gene expression analysis without a subsequent flight experiment. We could partially confirm the induction of cytochrome c oxidase subunit 5A, which we detected in experiment II. In addition, we analyzed the effect of the feeding mode (group feeding vs. single bee feeding) on data scattering and demonstrated that single bee feeding is superior to group feeding as it significantly reduces variability in gene expression. Based on the data, we thus hypothesize that the disruption of energy metabolism may be one reason for a prolongation of homing flight duration in neonicotinoid treated bees.


Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3173
Author(s):  
Elisa Palmioli ◽  
Cecilia Dall’Aglio ◽  
Michele Bellesi ◽  
Federico Maria Tardella ◽  
Sara Moscatelli ◽  
...  

Apelin (APLN) is an adipokine mainly produced by adipose tissue and related to an individual’s nutritional status as well as digestive apparatus functions. In this work, APLN and its receptor (APLNR) were investigated, by immunohistochemistry, in the abomasum and duodenum of 15 Comisana × Appenninica adult sheep reared in a semi-natural pasture. Organ samples were collected after maximum pasture flowering (M × F group) and after maximum pasture dryness (M × D group); the experimental group (E × p group) received a feed supplementation of 600 grams/day/head of barley and corn in addition to M × D group feeding. APLN and APLNR were identified in the lining epithelium and the fundic gland chief cells of the abomasum. APLNR was observed in the lining epithelium, in the crypts and the serotonin secreting cells of the duodenum. Similar reactivity was observed between the M × F and E × p groups, while the M × D group showed a lower intensity of immunostaining for both APLN and APLNR in all positive structures but the duodenal serotonin neuroendocrine cells. Hence, our findings show that the E × p group presents a picture quite overlapped with M × F and suggest that food supplementation has a maintaining effect on the apelinergic system expression in the investigated digestive tracts of the sheep.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 144-145
Author(s):  
Susan K Duckett ◽  
Jacob Cathcart ◽  
Austin Cathcart ◽  
Zach Dantzler ◽  
Hunter Dove ◽  
...  

Abstract Two experiments were conducted to evaluate use of the Super SmartFeeder (SSF; C-Lock, Inc.) for individual versus group supplementation of heifers grazing stockpiled, novel tall fescue. In experiment 1, Angus heifers (n = 64; 267 + 31.7 kg) had access to the SSF and were allowed 3.6 kg/d of grower supplement. Individual intake was recorded daily and analyzed to determine adoption. Twenty-four percent of the heifers did not adopt to individual SSF supplementation (P < 0.05). In experiment 2, heifers (n = 64; BW= 275 + 31.3 kg) were allotted, based on adoption to SSF, to supplementation system of group feeding (n = 2 reps/level; GRP) or SSF precision feeding (n =16/level; PRE) at two levels (0.5% or 1% of BW) for 127-d in a 2 x 2 factorial. Data were analyzed using Mixed procedure. The interaction between supplementation level and feeding system tended to be significant (P = 0.10) for overall ADG. Average daily gain was greater for 1% BW than 0.5% BW (0.81 vs. 0.47 kg/d) for PRE but did not differ for GRP (0.69 kg/d). Daily supplement dry matter intake differed (P < 0.05) by supplementation level and total BW gain was greater (P < 0.05) by 24.3 kg for 1% versus 0.5% supplementation level. Ultrasound ribeye area and fat thickness measures were greater (P < 0.05) for 1% BW supplement compared to 0.5% BW at the end of the 127d study. When PRE was analyzed independently, heifer BW differed (P < 0.05) on d 91, 117 and 127 between supplement levels. The correlation between individual heifer daily supplement intake and overall ADG for PRE was 0.68 (P < 0.0001). The use of technology to precisely control intake in a grazing system created greater divergence in growth by supplementation level compared to group feeding systems.


2021 ◽  
Vol 8 ◽  
Author(s):  
Chongyu Zhang ◽  
Chen Zhang ◽  
Yunpeng Wang ◽  
Meiyu Du ◽  
Guiguo Zhang ◽  
...  

Considerable evidence suggests that dietary energy levels and gut microbiota are pivotal for animal health and productivity. However, little information exists about the correlations among dietary energy level, performance, and the gut microbiota and metabolome of donkeys. The objective of this study was to investigate the mechanisms by which dietary energy content dictates the growth performance by modulating the intestinal microbiome and metabolome of donkeys. Thirty-six nine-month-old male Dezhou donkeys with similar body weights were randomly assigned to two groups fed low- or high-energy diets (LE or HE). The results showed that donkeys fed HE had increased (p < 0.05) the average daily gain (ADG) and feed efficiency (G/F) compared with those that received LE diet. The gut microbiota in both groups was dominated by the phyla Firmicutes and Bacteroidetes regardless of the dietary energy level. However, feeding HE to donkeys significantly decreased (p < 0.05) the ratio of Firmicutes to Bacteroidetes (F/B). Compared to the LE group, feeding HE specifically increased the abundances of unidentified_Prevotellaceae (p = 0.02) while decreasing the richness of unidentified_Ruminococcaceae (p = 0.05). Compared to the LE group, feeding the HE diet significantly (p < 0.05) upregulated certain metabolic pathways involving the aspartate metabolism and the urea cycle. In addition, the increased bacteria and metabolites in the HE-fed group exhibited a positive correlation with improved growth performance of donkeys. Taken together, feeding the HE diet increased the richness of Prevotellaceae and upregulated growth-related metabolic pathways, which may have contributed to the ameliorated growth performance of donkeys. Thus, it is a recommendable dietary strategy to feed HE diets to fattening donkeys for superior product performance and feed efficiency.


2021 ◽  
Vol 11 (3) ◽  
pp. 422-430
Author(s):  
Haiam Abd Elsalam Sayed ◽  
Mahmoud Metwaly El-Maghraby ◽  
Mohamed Mahmoud Elbadawy

The aim of the present study was to evaluate the effect of replacing concentrate feed (CFM) mixture with different levels of treated lemon pulp (TLP) on milk production and composition, nutrients digestibility, and blood parameters of Zaribi goats. A total of 24 Zaribi female goats at the end of the pregnant period were divided into three groups of eight according to live weight. Concentrate feed mixture and clover hay at a rate of 50:50 were offered twice a day. Treatedlemon pulp replaced CFM at rates 0%, 25%, and 50 % in diets of R1, R2, and R3 treatment groups, respectively. The feeding trial lasted 90 days. Dry matter digestibility and Nitrogen free extract digestibility were significantly raised by the increased level of TLP in groups R2 (72.37% and 70.36%) and R3 (72.28% and 70.30%), compared to (70.99% and 68.51%) in R1, respectively. The same trend was observed for organic matter digestibility (OMD), crude protein digestibility (CPD), crude fiber digestibility (CFD), and ether extract digestibility (EED) with R3, compared with either R1 or R2. However, there were insignificant differences between R1 and R2 in terms of OMD, CPD, and EED but CFD was significantly higher in R2, compared to R1. There was a significant increase in the milk yield by the increased level of TLP in the diet and for R2 (1448.4g/h/d) and R3(1558.7g/h/d), while it was reported as 1377.6 g/h/d in the control group. Feeding dams on the R3 diet had a significant effect on improving total antioxidant capacity by 63.5%, compared with control. The results of the present study indicated that the replacement of CFM by TLP decreased the costs of feed and increase milk production for the replacement level by 50% (R3). Moreover, no adverse effects were noticed on nutrients digestibility and blood parameters of the investigated samples.


Author(s):  
K. Kalyani ◽  
R.M.V. Prasad ◽  
Suresh Rathod ◽  
P. Jaya Laxmi

An investigation was carried out to study the housing and feeding management practices followed by the dairy farmers in five districts of Telangana state. From each district one mandal and from each mandal five villages were selected. The data was collected from 150 farmers through personal interview utilizing a schedule developed for the purpose. It was observed that most of the dairy farmers (59.33%) had medium herd size and 59.33% of farmers housed their animals beside their houses while 14.67% and 26% of the farmers housed the animals in a separate shed and below the shade of tree, respectively. Most of the animal sheds (66%) in the study area had mud flooring. Majority of farmers (52%) were not taking any measures to protect animals from extreme heat in summer. Grazing and group feeding were most commonly practised except in Rangareddy district where most of farmers (56.67%) followed individual feeding. Majority of the farmers from Medak (50%) and Mahaboobnagar (60%) districts were following one time feeding of their animals, whereas, most farmers from Vikarabad (66.67%) fed two times in a day. In Rangareddy and Nalgonda, majority (66.67% and 63.33%) of the farmers were offering feed three times. Most of the farmers were producing fodder by themselves in Vikarabad, Medak, Nalgonda and Mahaboobnagar districts and their percentages were 66.67%, 63.33% 93.33% and 86.67%, respectively. Most of the farmers of Rangareddy district were procuring fodder from outside.


2021 ◽  
Vol 15 (2) ◽  
pp. 101-106
Author(s):  
M. B. Musaev ◽  
V. V. Zashchepkina ◽  
Kh. Kh. Gadayev ◽  
Kh. Kh. Shakhbiyev

The purpose of the research is a commission test of the efficacy of the supramolecular complex of ivermectin at gastrointestinal strongylatoses of horses.Materials and methods. The commission experiment was performed in June 2019 in the North Caucasian Federal District of the Chechen Republic. The efficacy of the supramolecular complex of ivermectin was evaluated on 20 horses of different breeds in the Seradin Equestrian Center. To determine the rate of gastrointestinal nematode infection in horses, we collected fresh feces in the morning, made tissue imprints from perianal folds and examined them by the Fülleborn's method using a saturated solution of sodium chloride.Results and discussion. The coproovoscopic examination determined a 100% infection of horses with Strongylates and 40.0% infection with Oxyuris sp., with 488.7±24.4 strongylates eggs and 16.9±0.84 Oxyuris sp. eggs found in 1 g of feces, respectively. Seven and 15 days after the supramolecular complex of ivermectin administered to the horses from the first test group at a dose of 0.15 mg/kg for the active substance (7.5 mg/kg for the drug) in a mixture with mixed feed, we did not find any Strongylata eggs or Oxyuris sp. eggs in feces or on tissue imprints. Insufficient efficacy was obtained in the horses from the second test group after being treated with the active substance of ivermectin at a dose of 0.15 mg/kg. The strongylate egg number decreased on average by 244.3±12.21, namely, by 50.1%, and no Oxyuris sp. eggs were found. The efficacy against Oxyuris sp. was 100%. The horses consumed the mixture of drugs with feed readily. There were no side effects after the drugs applied. Thus, we established the high efficacy of the supramolecular complex of ivermectin against gastrointestinal strongylatoses, and the convenience of its use in a mixture with feed to untamed herd horses individually and by group feeding. 


2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 115-116
Author(s):  
Brad J Heins ◽  
Kirsten Sharpe

Abstract The objective of this study was to determine growth and health of Limousin crossbred dairy-beef calves fed alternative milk allowances in an automated group feeding system. The study was conducted at the University of Minnesota West Central Research and Outreach Center’s, Morris, MN dairy. Forty-eight Limousin-sired crossbred calves were assigned to feeding groups by birth order during two calving seasons from September to December 2019 and March to July 2020. Calves were introduced to the Holm & Laue HL100 Programmable Calf Feeder (Holm & Laue GmbH & Co KG, Westerronfeld, Germany) at 5 d and were randomly assigned to one of two treatments. Treatments for calves were 8 L/d (8L) or ad libitum (AL) milk allowance. Calves were weaned from the automated feeder at 56 d. Data were analyzed using PROC MIXED of SAS. Independent variables for analyses were the fixed effects of season of birth, sex of calf (M or F), treatment group and sex of calf nested within season of birth was a random effect. No differences (P > 0.05) were found between 8L or AL groups for birth weight. The AL calves (109.4 kg) had higher (P < 0.01) weaning weight than 8L calves (95.3 kg). Furthermore, the AL calves had higher (P < 0.01) hip height (95.6 cm) and greater (P < 0.01) heart girth (110.2) compared with 8L calves (93.8 cm and 104.8 cm, respectively). The AL calves (1.14 kg/d) had higher average daily gain at weaning compared to 8L calves (0.91 kg/d). Males calves were not different (P = 0.39) for average daily gain compared to female calves (1.07 kg/d versus 0.98 kg/d). Drinking speeds of AL calves were lesser (P < 0.05; 1,222 mL/min) than the 8L calves (1,453 mL/min). The AL calves and 8L calves were not different for hygiene scores or health scores. The results from this study indicate advantages to feeding dairy-beef calves ad libitum whole milk during the pre-weaning period.


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