Genetic deletion of vesicular monoamine transporter-2 (VMAT2) reduces dopamine transporter activity in mesencephalic neurons in primary culture

2007 ◽  
Vol 51 (2-4) ◽  
pp. 237-244 ◽  
Author(s):  
Hideko Yamamoto ◽  
Etsuko Kamegaya ◽  
Yoko Hagino ◽  
Kazuhide Imai ◽  
Akihiro Fujikawa ◽  
...  
Keyword(s):  
Primary Culture ◽  
Dopamine Transporter ◽  
Vesicular Monoamine Transporter ◽  
Monoamine Transporter ◽  
Vesicular Monoamine Transporter 2 ◽  
Genetic Deletion

scholarly journals Age-dependent differences in dopamine transporter and vesicular monoamine transporter-2 function and their implications for methamphetamine neurotoxicity

Synapse ◽  
2008 ◽  
Vol 63 (2) ◽  
pp. 147-151 ◽  
Author(s):  
Trent J. Volz ◽  
Sarah J. Farnsworth ◽  
Shane D. Rowley ◽  
Glen R. Hanson ◽  
Annette E. Fleckenstein
Keyword(s):  
Dopamine Transporter ◽  
Vesicular Monoamine Transporter ◽  
Monoamine Transporter ◽  
Vesicular Monoamine Transporter 2 ◽  
Age Dependent ◽  
Methamphetamine Neurotoxicity

scholarly journals Catecholamine Uptake, Storage, and Regulated Release by Ovarian Granulosa Cells

Endocrinology ◽  
2008 ◽  
Vol 149 (10) ◽  
pp. 4988-4996 ◽  
Author(s):  
Monika Greiner ◽  
Alfonso Paredes ◽  
Verónica Rey-Ares ◽  
Sabine Saller ◽  
Artur Mayerhofer ◽  
...  
Keyword(s):  
Dopamine Transporter ◽  
Granulosa Cells ◽  
Polycystic Ovary ◽  
Ovarian Follicles ◽  
Nerve Fibers ◽  
Vesicular Monoamine Transporter ◽  
Monoamine Transporter ◽  
Vesicular Monoamine Transporter 2 ◽  
Calcium Dependent ◽  
Sympathetic Nerve Fibers

Catecholamines present in the mammalian ovary are involved in many normal aspects of ovarian functions, including initial follicle growth, steroidogenesis, and pathological states such as polycystic ovary syndrome. Sympathetic nerve fibers are the largest source of norepinephrine (NE), but not the only one. Surgical denervation of the rat ovary reduces, but does not eliminate, the ovarian content of NE. The aim of this work was to explore which intraovarian cells may participate in the ovarian NE homeostasis and the mechanisms involved. It was found that denervated rat ovaries can take up NE and cocaine considerably, decreased its uptake, suggesting involvement of catecholamine transporters. Granulosa cells of rat ovarian follicles present dopamine transporter and NE transporter. Their functionality was confirmed in isolated rat granulosa cells while cocaine blocked the uptake of NE. Furthermore, the presence of the vesicular monoamine transporter 2, together with the exocytotic protein (synaptosome-associated protein of 25 kDa) in granulosa cells, implies catecholamine storage and regulated release. Regulated calcium-dependent release of NE was shown after depolarization by potassium, implying all neuron-like cellular machinery in granulosa cells. These results in rats may be of relevance for the human ovary because dopamine transporter, NE transporter, vesicular monoamine transporter 2, and synaptosome-associated protein of 25-kDa protein and mRNA are found in human ovarian follicles and/or isolated granulosa cells. Thus, ovarian nonneuronal granulosa cells, after taking up catecholamines, can serve as an intraovarian catecholamine-storing compartment, releasing them in a regulated way. This suggests a more complex involvement of catecholamines in ovarian functions as is currently being recognized.

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