Change and translocation of HSP60 in gerbil hippocampal CA1 region induced by transient ischemia and its protective effect against ischemic damage

2007 ◽  
Vol 58 ◽  
pp. S241
Author(s):  
In Koo Hwang ◽  
Ki-Yeon Yoo ◽  
Jung Hoon Choi ◽  
In Se Lee ◽  
Yeo Sung Yoon ◽  
...  
Pathobiology ◽  
2009 ◽  
Vol 76 (4) ◽  
pp. 204-211 ◽  
Author(s):  
Hiro Eda ◽  
Shigeru Sato ◽  
Yoshihiro Sasaki ◽  
Akiko Adachi ◽  
Mohammad Ghazizadeh

2002 ◽  
Vol 96 (3) ◽  
pp. 705-710 ◽  
Author(s):  
Ryosuke Nakayama ◽  
Toshiyuki Yano ◽  
Kazuo Ushijima ◽  
Eiji Abe ◽  
Hidenori Terasaki

Background Excessive extracellular glutamate produced by cerebral ischemia has been proposed to initiate the cascade toward neuronal cell death. Changes in extracellular glutamate concentration are closely linked to changes in intracellular calcium ion concentration. Dantrolene inhibits calcium release from intracellular calcium stores. In this study, the authors investigated the effects of dantrolene on extracellular glutamate accumulation and neuronal degeneration in a rat model of transient global forebrain ischemia. Methods Male Wistar rats weighing 230-290 g were anesthetized with halothane in nitrous oxide-oxygen and were subjected to 10 min of transient forebrain ischemia using a four-vessel occlusion technique. Fifteen minutes before ischemic injury, dantrolene sodium (5 mm), dimethyl sulfoxide as a vehicle for dantrolene, or artificial cerebrospinal fluid as a control was intracerebroventricularly administered (n = 8 in each group). In the hippocampal CA1 subfield, the extracellular glutamate concentration in vivo was measured during the periischemic period with a microdialysis biosensor, and the number of intact neurons was evaluated on day 7 after reperfusion. Results Both dantrolene and dimethyl sulfoxide significantly reduced the ischemia-induced increase in glutamate concentration to a similar extent, i.e., by 53 and 51%, respectively, compared with artificial cerebrospinal fluid (P < 0.01). The number of intact hippocampal CA1 neurons (mean +/- SD; cells/mm) in dantrolene-treated rats (78 +/- 21) was significantly higher than that in artificial cerebrospinal fluid- (35 +/- 14; P < 0.001) and dimethyl sulfoxide-treated (56 +/- 11; P < 0.05) animals. Dimethyl sulfoxide also significantly increased the number of preserved neurons in comparison with artificial cerebrospinal fluid (P < 0.05). Conclusions Intracerebroventricular dantrolene prevents delayed neuronal loss in the rat hippocampal CA1 region subjected to transient ischemia; however, this neuroprotection cannot be accounted for only by the reduced concentrations of extracellular glutamate during ischemia.


2007 ◽  
Vol 208 (2) ◽  
pp. 247-256 ◽  
Author(s):  
In Koo Hwang ◽  
Hee Cheol Ahn ◽  
Ki-Yeon Yoo ◽  
Jae-Yong Lee ◽  
Hong-Won Suh ◽  
...  

2009 ◽  
Vol 1263 ◽  
pp. 134-142 ◽  
Author(s):  
In Koo Hwang ◽  
Ki-Yeon Yoo ◽  
Sun Shin Yi ◽  
Il Yong Kim ◽  
Hye Sook Hwang ◽  
...  

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