Activation of the insulin-signaling pathway in sciatic nerve and hippocampus of type 1 diabetic rats

Diabetes mellitus, a disease arising by the deficiency of insulin hormone or its inability of usage, affects carbohydrate, lipid and protein metabolism, and destruct variety of the tissues. A strong antioxidant and anti-inflammatory agent; resveratrol has a high potential to prevent or treat the pathogenesis of diseases. This study was conducted to reveal the relationship between diabetes-induced oxidative stress and tissue inflammation with changes in antioxidant enzymes (cat, sod, gpx, and gst) and the components of insulin signaling pathway (insulin Rβ, irs-1, pi3k, akt, mtor) in kidney tissues. Additionally, the effects of resveratrol on these parameters were evaluated. Male Wistar rats were randomly divided into four groups; (1) control/vehicle; (2) control/20 mg/kg resveratrol; (3) diabetic/vehicle; (4) diabetic/20 mg/kg resveratrol. Results demonstrated down-regulation of antioxidant enzymes in the kidney tissues of diabetic rats and this situation was devoted partially to the reduced expression of nfκb. Moreover, the components of renal insulin signaling elements were up-regulated in diabetic rats, and resveratrol treatment decreased this sensitization towards the control state. In conclusion, resveratrol improved diabetes-induced renal oxidative stress and inflammation partly due to healing action on renal antioxidant enzymes and insulin signaling pathway components.

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Modulation of Renal Insulin Signaling Pathway and Antioxidant Enzymes with Streptozotocin-Induced Diabetes: Effects of Resveratrol

Medicina ◽

10.3390/medicina55010003 ◽

2018 ◽

Vol 55 (1) ◽

pp. 3 ◽

Cited By ~ 3

Author(s):

Gökhan Sadi ◽

Gamze Şahin ◽

Aykut Bostanci

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Antioxidant Enzymes ◽

Signaling Pathway ◽

Insulin Signaling ◽

Diabetic Rats ◽

Insulin Signaling Pathway ◽

Enzymatic Antioxidants ◽

Gene And Protein Expression ◽

Male Wistar Rats

Background and objectives: Diabetes mellitus is a disease of insulin deficiency or its inability of usage by the target tissues leading to impairment of carbohydrate, lipid, and protein metabolisms. Resveratrol, having robust anti-inflammatory and anti-oxidant properties, has a high potential to treat or prevent the pathogenesis of diseases. This study was conducted to reveal the relationship between diabetes-induced oxidative stress and tissue inflammation with changes in main enzymatic antioxidants (cat, sod, gpx, and gst) and the components of the insulin signaling pathway (insulin Rβ, irs-1, pi3k, akt, mtor) in kidney tissues. Additionally, the effects of resveratrol on these parameters were evaluated. Materials and Methods: Male Wistar rats were randomly divided into four groups; (1) control/vehicle; (2) control/20 mg/kg resveratrol; (3) diabetic/vehicle; (4) diabetic/20 mg/kg resveratrol. Gene and protein expressions of antioxidant enzymes and insulin signaling elements were evaluated in renal tissues. Results: Downregulation of antioxidant enzymes’ gene expression in the kidney tissues of diabetic rats was demonstrated and this situation was devoted partially to the reduced gene expression of nfκb. Moreover, the components of renal insulin signaling elements were upregulated at both gene and protein expression levels in diabetic rats, and resveratrol treatment decreased this sensitization towards the control state. Conclusion: Resveratrol partially improved diabetes-induced renal oxidative stress and inflammation due to healing action on renal antioxidant enzymes and insulin signaling pathway components.

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