A method for spatio-temporal characterization of pulse beams with an all-optical image sensor

2022 ◽  
Vol 151 ◽  
pp. 106872
Author(s):  
Yan Song ◽  
Bodong Peng ◽  
Qing Xu ◽  
Guzhou Song ◽  
Zhiqin Yue ◽  
...  
Keyword(s):  
Image Sensor ◽  
Optical Image ◽  
All Optical ◽  
Spatio Temporal

scholarly journals Fluorescence Fluctuation Spectroscopy enables quantification of potassium channel subunit dynamics and stoichiometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Giulia Tedeschi ◽  
Lorenzo Scipioni ◽  
Maria Papanikolaou ◽  
Geoffrey W. Abbott ◽  
Michelle A. Digman
Keyword(s):  
Plasma Membrane ◽  
Protein Diffusion ◽  
Ion Diffusion ◽  
Fluorescence Fluctuation Spectroscopy ◽  
Biophysical Characterization ◽  
Kv Channels ◽  
Subunit Stoichiometry ◽  
Spatio Temporal ◽  
Voltage Sensing Domain

AbstractVoltage-gated potassium (Kv) channels are a family of membrane proteins that facilitate K+ ion diffusion across the plasma membrane, regulating both resting and action potentials. Kv channels comprise four pore-forming α subunits, each with a voltage sensing domain, and they are regulated by interaction with β subunits such as those belonging to the KCNE family. Here we conducted a comprehensive biophysical characterization of stoichiometry and protein diffusion across the plasma membrane of the epithelial KCNQ1-KCNE2 complex, combining total internal reflection fluorescence (TIRF) microscopy and a series of complementary Fluorescence Fluctuation Spectroscopy (FFS) techniques. Using this approach, we found that KCNQ1-KCNE2 has a predominant 4:4 stoichiometry, while non-bound KCNE2 subunits are mostly present as dimers in the plasma membrane. At the same time, we identified unique spatio-temporal diffusion modalities and nano-environment organization for each channel subunit. These findings improve our understanding of KCNQ1-KCNE2 channel function and suggest strategies for elucidating the subunit stoichiometry and forces directing localization and diffusion of ion channel complexes in general.

SPATIO-TEMPORAL CHARACTERIZATION of litter at a touristic sandy beach in south Brazil

2021 ◽  
pp. 116927
Author(s):  
Bruna de Ramos ◽  
Melanie Vianna Alencar ◽  
Fábio Lameiro Rodrigues ◽  
Ana Luzia de Figueiredo Lacerda ◽  
Maíra Carneiro Proietti
Keyword(s):  
Sandy Beach ◽  
Spatio Temporal ◽  
South Brazil

Spatio-temporal characterization of optical waveforms

2017 ◽  
Author(s):  
T. Witting ◽  
G. Greening ◽  
D. Walke ◽  
P. Matia-Hernando ◽  
T. Barillot ◽  
...  
Keyword(s):  
Spatio Temporal

scholarly journals Spatio-temporal characterization of landscape fire in relation to anthropogenic activity and climatic variability over the Western Himalaya, India

2021 ◽  
Vol 58 (2) ◽  
pp. 281-299
Author(s):  
Somnath Bar ◽  
Bikash Ranjan Parida ◽  
Gareth Roberts ◽  
Arvind Chandra Pandey ◽  
Prasenjit Acharya ◽  
...  
Keyword(s):  
Climatic Variability ◽  
Western Himalaya ◽  
Anthropogenic Activity ◽  
Spatio Temporal

scholarly journals Multiphoton Optical Image Guided Spectroscopy Method for Characterization of Collagen-Based Materials Modified by Glycation

2011 ◽  
Vol 83 (1) ◽  
pp. 200-206 ◽  
Author(s):  
Yu-Jer Hwang ◽  
Joseph Granelli ◽  
Julia G. Lyubovitsky
Keyword(s):  
Optical Image ◽  
Spectroscopy Method ◽  
Image Guided

Damage Detection in Flexible Propeller Beam Structures by Exploiting Impact-Induced Coupled Acceleration Signals

2011 ◽  
Author(s):  
Ioannis T. Georgiou
Keyword(s):  
Data Driven ◽  
Distributed Information ◽  
Modal Expansion ◽  
Shape Difference ◽  
Spatio Temporal ◽  
Temporal Sample ◽  
High Level ◽  
Proper Orthogonal ◽  
Acceleration Signals

A local damage at the tip of a composite propeller is diagnosed by properly comparing its impact-induced free coupled dynamics to that of a pristine wooden propeller of the same size and shape. This is accomplished by creating indirectly via collocated measurements distributed information for the coupled acceleration field of the propellers. The powerful data-driven modal expansion analysis delivered by the Proper Orthogonal Decomposition (POD) Transform reveals that ensembles of impact-induced collocated coupled experimental acceleration signals are underlined by a high level of spatio-temporal coherence. Thus they furnish a valuable spatio-temporal sample of coupled response induced by a point impulse. In view of this fact, a tri-axial sensor was placed on the propeller hub to collect collocated coupled acceleration signals induced via modal hammer nondestructive impacts and thus obtained a reduced order characterization of the coupled free dynamics. This experimental data-driven analysis reveals that the in-plane unit components of the POD modes for both propellers have similar shapes-nearly identical. For the damaged propeller this POD shape-difference is quite pronounced. The shapes of the POD modes are used to compute indices of difference reflecting directly damage. At the first POD energy level, the shape-difference indices of the damaged composite propeller are quite larger than those of the pristine wooden propeller.

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