On the quantitative optical properties of Au nanoparticles embedded in biological tissue phantoms

Extensive research in the area of optical sensing for medical diagnostics requires development of tissue phantoms with optical properties similar to those of living human tissues. Development and improvement of in vivo optical measurement systems requires the use of stable tissue phantoms with known characteristics, which are mainly used for calibration of such systems and testing their performance over time. Optical and mechanical properties of phantoms depend on their purpose. Nevertheless, they must accurately simulate specific tissues they are supposed to mimic. Many tissues and organs including head possess a multi-layered structure, with specific optical properties of each layer. However, such a structure is not always addressed in the present-day phantoms. In this paper, we focus on the development of a plain-parallel multi-layered phantom with optical properties (reduced scattering coefficient [Formula: see text] and absorption coefficient μa) corresponding to the human head layers, such as skin, skull, and gray and white matter of the brain tissue. The phantom is intended for use in noninvasive diffuse near-infrared spectroscopy (NIRS) of human brain. Optical parameters of the fabricated phantoms are reconstructed using spectrophotometry and inverse adding-doubling calculation method. The results show that polyvinyl chloride-plastisol (PVCP) and zinc oxide ( ZnO ) nanoparticles are suitable materials for fabrication of tissue mimicking phantoms with controlled scattering properties. Good matching was found between optical properties of phantoms and the corresponding values found in the literature.

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Discovering new 3D bioprinting applications: Analyzing the case of optical tissue phantoms

International Journal of Bioprinting ◽

10.18063/ijb.v5i1.178 ◽

2018 ◽

Vol 5 (1) ◽

Author(s):

Marisela Rodriguez-Salvador

Keyword(s):

Optical Properties ◽

3D Printing ◽

Clinical Training ◽

Three Dimensional ◽

Biological Tissues ◽

3D Bioprinting ◽

Technology Intelligence ◽

Biomedical Device ◽

Tissue Phantoms ◽

Scientific Papers

Optical tissue phantoms enable to mimic the optical properties of biological tissues for biomedical device calibration, new equipment validation, and clinical training for the detection, and treatment of diseases. Unfortunately, current methods for their development present some problems, such as a lack of repeatability in their optical properties. Where the use of three-dimensional (3D) printing or 3D bioprinting could address these issues. This paper aims to evaluate the use of this technology in the development of optical tissue phantoms. A competitive technology intelligence methodology was applied by analyzing Scopus, Web of Science, and patents from January 1, 2000, to July 31, 2018. The main trends regarding methods, materials, and uses, as well as predominant countries, institutions, and journals, were determined. The results revealed that, while 3D printing is already employed (in total, 108 scientific papers and 18 patent families were identified), 3D bioprinting is not yet applied for optical tissue phantoms. Nevertheless, it is expected to have significant growth. This research gives biomedical scientists a new window of opportunity for exploring the use of 3D bioprinting in a new area that may support testing of new equipment and development of techniques for the diagnosis and treatment of diseases.

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Photoconductivity and optical properties of silicon coated by thin TiO2 film in situ doped by Au nanoparticles

physica status solidi (a) ◽

10.1002/pssa.201200804 ◽

2013 ◽

Vol 210 (4) ◽

pp. 687-694 ◽

Cited By ~ 5

Author(s):

Jan Amaru Töfflinger ◽

Esteban Pedrueza ◽

Vladimir Chirvony ◽

Caspar Leendertz ◽

Raúl García-Calzada ◽

...

Keyword(s):

Optical Properties ◽

Tio2 Film ◽

Au Nanoparticles

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Nonlinear optical properties of Au nanoparticles on indium–tin oxide substrate

Nanotechnology ◽

10.1088/0957-4484/16/9/046 ◽

2005 ◽

Vol 16 (9) ◽

pp. 1687-1692 ◽

Cited By ~ 62

Author(s):

I V Kityk ◽

J Ebothé ◽

I Fuks-Janczarek ◽

A Ali Umar ◽

K Kobayashi ◽

...

Keyword(s):

Optical Properties ◽

Tin Oxide ◽

Indium Tin Oxide ◽

Nonlinear Optical ◽

Au Nanoparticles ◽

Nonlinear Optical Properties ◽

Oxide Substrate ◽

Indium Tin Oxide Substrate

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Measurement and Thermal Dependence of Biological Tissue Optical Properties

Theory and Applications of Heat Transfer in Humans ◽

10.1002/9781119127420.ch18 ◽

2018 ◽

pp. 355-378 ◽

Cited By ~ 1

Author(s):

William C. Vogt ◽

Jennifer K. Barton ◽

Anant Agrawal ◽

T. Joshua Pfefer

Keyword(s):

Optical Properties ◽

Biological Tissue ◽

Thermal Dependence ◽

Tissue Optical Properties

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Absorption by water increases fluorescence image contrast of biological tissue in the shortwave infrared

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1803210115 ◽

2018 ◽

Vol 115 (37) ◽

pp. 9080-9085 ◽

Cited By ~ 19

Author(s):

Jessica A. Carr ◽

Marianne Aellen ◽

Daniel Franke ◽

Peter T. C. So ◽

Oliver T. Bruns ◽

...

Keyword(s):

Penetration Depth ◽

Fluorescence Imaging ◽

Biological Tissue ◽

Ex Vivo ◽

Scattered Light ◽

Image Contrast ◽

Fluorescence Image ◽

Tissue Phantoms ◽

Shortwave Infrared

Recent technology developments have expanded the wavelength window for biological fluorescence imaging into the shortwave infrared. We show here a mechanistic understanding of how drastic changes in fluorescence imaging contrast can arise from slight changes of imaging wavelength in the shortwave infrared. We demonstrate, in 3D tissue phantoms and in vivo in mice, that light absorption by water within biological tissue increases image contrast due to attenuation of background and highly scattered light. Wavelengths of strong tissue absorption have conventionally been avoided in fluorescence imaging to maximize photon penetration depth and photon collection, yet we demonstrate that imaging at the peak absorbance of water (near 1,450 nm) results in the highest image contrast in the shortwave infrared. Furthermore, we show, through microscopy of highly labeled ex vivo biological tissue, that the contrast improvement from water absorption enables resolution of deeper structures, resulting in a higher imaging penetration depth. We then illustrate these findings in a theoretical model. Our results suggest that the wavelength-dependent absorptivity of water is the dominant optical property contributing to image contrast, and is therefore crucial for determining the optimal imaging window in the infrared.

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