Förster resonance energy transfer (FRET)-Labeled nanoprobe enables real-time diagnosis of pancreatic juice activation due to postoperative pancreatic fistula

Pancreatology ◽  
2020 ◽  
Vol 20 (5) ◽  
pp. 960-967
Author(s):  
Satoshi Mizutani ◽  
Masaharu Murata ◽  
Nobuhiko Taniai ◽  
Makoto Sukegawa ◽  
Ryosuke Nakata ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Pancreatic Fistula ◽  
Real Time ◽  
Pancreatic Juice ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Postoperative Pancreatic Fistula ◽  
Förster Resonance Energy Transfer ◽  
Time Diagnosis ◽  
Förster Resonance

A novel robust quantitative Förster resonance energy transfer assay for protease SENP2 kinetics determination against its all natural substrates

2015 ◽  
Vol 11 (12) ◽  
pp. 3407-3414 ◽  
Author(s):  
Yan Liu ◽  
Yali Shen ◽  
Shasha Zheng ◽  
Jiayu Liao
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Post Translational Modification ◽  
Kinetics Parameters ◽  
Multiple Processes ◽  
Förster Resonance

SUMOylation (the process of adding the SUMO [small ubiquitin-like modifier] to substrates) is an important post-translational modification of critical proteins in multiple processes. The kinetics parameters of pre-SUMO1-3 by its protease SENP2 is determined by a quantitative FRET assay in real time.

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