scholarly journals Internal Calibration Förster Resonance Energy Transfer Assay: A Real-Time Approach for Determining Protease Kinetics

Sensors ◽  
2013 ◽  
Vol 13 (4) ◽  
pp. 4553-4570 ◽  
Author(s):  
Ling Jiang ◽  
Yan Liu ◽  
Yang Song ◽  
Amanda Saavedra ◽  
Songqin Pan ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Internal Calibration ◽  
Förster Resonance

A novel robust quantitative Förster resonance energy transfer assay for protease SENP2 kinetics determination against its all natural substrates

2015 ◽  
Vol 11 (12) ◽  
pp. 3407-3414 ◽  
Author(s):  
Yan Liu ◽  
Yali Shen ◽  
Shasha Zheng ◽  
Jiayu Liao
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Post Translational Modification ◽  
Kinetics Parameters ◽  
Multiple Processes ◽  
Förster Resonance

SUMOylation (the process of adding the SUMO [small ubiquitin-like modifier] to substrates) is an important post-translational modification of critical proteins in multiple processes. The kinetics parameters of pre-SUMO1-3 by its protease SENP2 is determined by a quantitative FRET assay in real time.

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