scholarly journals Elastic deformations of the rotary double motor of single FoF1-ATP synthases detected in real time by Förster resonance energy transfer

2012 ◽  
Vol 1817 (10) ◽  
pp. 1722-1731 ◽  
Author(s):  
Stefan Ernst ◽  
Monika G. Düser ◽  
Nawid Zarrabi ◽  
Stanley D. Dunn ◽  
Michael Börsch
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Elastic Deformations ◽  
Förster Resonance ◽  
Atp Synthases

A novel robust quantitative Förster resonance energy transfer assay for protease SENP2 kinetics determination against its all natural substrates

2015 ◽  
Vol 11 (12) ◽  
pp. 3407-3414 ◽  
Author(s):  
Yan Liu ◽  
Yali Shen ◽  
Shasha Zheng ◽  
Jiayu Liao
Keyword(s):  
Energy Transfer ◽  
Real Time ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Forster Resonance Energy Transfer ◽  
Förster Resonance Energy Transfer ◽  
Post Translational Modification ◽  
Kinetics Parameters ◽  
Multiple Processes ◽  
Förster Resonance

SUMOylation (the process of adding the SUMO [small ubiquitin-like modifier] to substrates) is an important post-translational modification of critical proteins in multiple processes. The kinetics parameters of pre-SUMO1-3 by its protease SENP2 is determined by a quantitative FRET assay in real time.

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