Evaluation of the sysmex XE 5000 body fluid module in for determining cell counts in sterile body fluids including cerebrospinal fluid

AbstractBackground:Cellular analysis in cerebrospinal fluid (CSF) provides important diagnostic information in various medical conditions. The aim of this study was to evaluate the application of Mindray BC-6800 body fluid (BF) mode in cytometric analysis of CSF compared to light microscopy (LM).Methods:One hundred and twenty-nine consecutive CSF samples were analyzed by BC-6800-BF mode as well as by LM. The study also included limits of blank (LoB), limit of detection (LoD), limit of quantitation (LoQ), carryover and linearity.ResultsWhite blood cells LoQ was 4.0×10Conclusions:BC-6800-BF offers rapid and accurate counts in clinically relevant concentration ranges, replacing LM for most samples. However, in samples with abnormal cell counts or with abnormal white blood cell differential scattergrams the need to microscopic review for a correct clinical outcome remains.

Download Full-text

Evaluation of body fluid mode of Sysmex XN-9000 for white blood cell counts in cerebrospinal fluid

Journal of Laboratory and Precision Medicine ◽

10.21037/jlpm.2018.02.01 ◽

2018 ◽

Vol 3 ◽

pp. 22-22 ◽

Cited By ~ 1

Author(s):

Vincenzo Roccaforte ◽

Massimo Daves ◽

Vanessa Proserpio ◽

Flavia Sciarini ◽

Rosella Sangiorgio ◽

...

Keyword(s):

Cerebrospinal Fluid ◽

Blood Cell ◽

White Blood Cell ◽

Body Fluid ◽

Cell Counts ◽

Blood Cell Counts ◽

White Blood Cell Counts

Download Full-text

Detection of Malignancy in Body Fluids: A Comparison of the Hematology and Cytology Laboratories

Archives of Pathology & Laboratory Medicine ◽

10.5858/arpa.2013-0295-oa ◽

2014 ◽

Vol 138 (5) ◽

pp. 651-657 ◽

Cited By ~ 3

Author(s):

Jaclyn L. Jerz ◽

Rachel E. Donohue ◽

Rayomond R. Mody ◽

Mary R. Schwartz ◽

Dina R. Mody ◽

...

Keyword(s):

Quality Assurance ◽

Retrospective Review ◽

Body Fluid ◽

Initial Study ◽

Body Fluids ◽

Cytologic Examination ◽

Cell Counts ◽

Flow Cytometric ◽

Increased Sensitivity ◽

Medical Technologists

Context.—Body fluids submitted to the hematology laboratory for cell counts may also be examined for the presence of malignancy. Previous studies evaluating the hematology laboratory's performance at detecting malignancy in body fluids have reached conflicting conclusions. Objective.—To investigate the hematology laboratory's ability to detect malignancy in body fluids by comparison with cytology. Design.—Retrospective analysis of 414 body fluid samples during an 18-month period, with introduction of new quality assurance measures after the first 210 cases. If no concurrent cytology was ordered, results were compared with recent previous and/or subsequent cytologic, histologic, or flow cytometric diagnoses. Results.—Of the initial 210 cases, the hematology laboratory detected 3 of 13 malignancies diagnosed by concurrent cytology (23% sensitivity), with no false-positives (100% specificity). Malignancy was not identified on retrospective review of the hematology slides in the 10 discrepant cases. After the initial study, educational sessions on morphology for the medical technologists and a more thorough hematology-cytology correlation policy were implemented. The subsequent 204 hematology laboratory cases had increased sensitivity for the detection of malignancy (60%; 6 of 10). Definitive features of malignancy were seen in only one discrepant hematology laboratory slide on retrospective review. This case had not been flagged for hematopathologist review. None of the discrepancies before or after implementation of the additional quality assurance measures impacted patient care. Conclusions.—Body fluid processing by the hematology laboratory is not optimized for the detection of malignancy. Concurrent cytologic examination is critical for the detection of malignancy, and needs to be considered as cost-saving measures are increasingly implemented.

Download Full-text

Validation of the body fluid module on the new Sysmex XN-1000 for counting blood cells in cerebrospinal fluid and other body fluids

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2011-0927 ◽

2012 ◽

Vol 50 (10) ◽

Cited By ~ 41

Author(s):

Chérina Fleming ◽

Rob Brouwer ◽

Jan Lindemans ◽

Robert de Jonge

Keyword(s):

Cerebrospinal Fluid ◽

Blood Cells ◽

Body Fluid ◽

Body Fluids ◽

The Body

Download Full-text

Detection of bloodstains using attenuated total reflectance-Fourier transform infrared spectroscopy supported with PCA and PCA–LDA

Medicine Science and the Law ◽

10.1177/00258024211010926 ◽

2021 ◽

pp. 002580242110109

Author(s):

Sweety Sharma ◽

Rito Chophi ◽

Jaskirandeep Kaur Jossan ◽

Rajinder Singh

Keyword(s):

Fourier Transform ◽

Ftir Spectroscopy ◽

Body Fluid ◽

Due Process ◽

Dna Analysis ◽

Fourier Transform Infrared ◽

Body Fluids ◽

Attenuated Total Reflectance ◽

Total Reflectance ◽

Non Destructive

The most important task in a criminal investigation is to detect and identify the recovered biological stains beyond reasonable scientific doubt and preserve the sample for further DNA analysis. In the light of this fact, many presumptive and confirmatory tests are routinely employed in the forensic laboratories to determine the type of body fluid. However, the currently used techniques are specific to one type of body fluid and hence it cannot be utilized to differentiate multiple body fluids. Moreover, these tests consume the samples in due process, and thus it becomes a great limitation especially considering the fact that samples are recovered in minute quantity in forensic cases. Therefore, such limitations necessitate the use of non-destructive techniques that can be applied simultaneously to all types of bodily fluids and allow sample preservation for further analysis. In the current work, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy has been used to circumvent the aforementioned limitations. The important factors which could influence the detection of blood such as the effect of substrates, washing/chemical treatment, ageing, and dilution limits on the analysis of blood have been analysed. In addition, blood discrimination from non-blood substance (biological and non-biological in nature) has also been studied. Chemometric technique that is PCA–LDA has been used to discriminate blood from other body fluids and it resulted in 100% accurate classification. Furthermore, blood and non-blood substances including fake blood have also been classified into separate clusters with a 100% accuracy, sensitivity, and specificity. All-inclusive, this preliminary study substantiates the potential application of ATR-FTIR spectroscopy for the non-destructive identification of blood traces in simulated forensic casework conditions with 0% rate of false classification.

Download Full-text

Applicability and performance of EUCAST’s rapid antimicrobial susceptibility testing (RAST) on primarily sterile body fluids in blood culture bottles in laboratory routine with total lab automation

European Journal of Clinical Microbiology & Infectious Diseases ◽

10.1007/s10096-020-04146-6 ◽

2021 ◽

Author(s):

Jasmin Kaur Jasuja ◽

Stefan Zimmermann ◽

Irene Burckhardt

Keyword(s):

Blood Culture ◽

Susceptibility Testing ◽

Reference Method ◽

Body Fluids ◽

E Coli ◽

Mueller Hinton ◽

Mueller Hinton Agar ◽

And Performance ◽

Sterile Body Fluids ◽

Better Than

AbstractOptimisation of microbiological diagnostics in primarily sterile body fluids is required. Our objective was to apply EUCAST’s RAST on primarily sterile body fluids in blood culture bottles with total lab automation (TLA) and to compare results to our reference method Vitek2 in order to report susceptibility results earlier. Positive blood culture bottles (BACTEC™ Aerobic/Anaerobic/PEDS) inoculated with primarily sterile body fluids were semi-automatically subcultured onto Columbia 5% SB agar, chocolate agar, MacConkey agar, Schaedler/KV agar and Mueller-Hinton agar. On latter, cefoxitin, ampicillin, vancomycin, piperacillin/tazobactam, meropenem and ciprofloxacin were added. After 6 h, subcultures and RAST were imaged and MALDI-TOF MS was performed. Zone sizes were digitally measured and interpreted following RAST breakpoints for blood cultures. MIC values were determined using Vitek2 panels. During a 1-year period, 197 Staphylococcus aureus, 91 Enterococcus spp., 38 Escherichia coli, 11 Klebsiella pneumoniae and 8 Pseudomonas aeruginosa were found. Categorical agreement between RAST and MIC was 96.5%. Comparison showed no very major errors, 2/7 (28.6%) and 1/7 (14.3%) of major errors for P. aeruginosa and meropenem and ciprofloxacin, 1/9 (11.1%) for K. pneumoniae and ciprofloxacin, 4/69 (7.0%) and 3/43 (5.8%) for Enterococcus spp. and vancomycin and ampicillin, respectively. Minor errors for P. aeruginosa and meropenem (1/8; 12.8%) and for E. coli and ciprofloxacin (2/29; 6.5%) were found. 30/550 RAST measurements were within area of technical uncertainty. RAST is applicable and performs well for primarily sterile body fluids in blood culture bottles, partially better than blood-based RAST. Official EUCAST evaluation is needed.

Download Full-text