Optimized expression of soluble cyclomaltodextrinase of thermophilic origin in Escherichia coli by using a soluble fusion-tag and by tuning of inducer concentration

2005 ◽  
Vol 39 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Pernilla Turner ◽  
Olle Holst ◽  
Eva Nordberg Karlsson
Keyword(s):  
Escherichia Coli ◽  
Fusion Tag ◽  
Inducer Concentration

scholarly journals Addition of an N-Terminal Poly-Glutamate Fusion Tag Improves Solubility and Production of Recombinant TAT-Cre Recombinase in Escherichia coli

2020 ◽  
Vol 30 (1) ◽  
pp. 109-117
Author(s):  
A-Hyeon Kim ◽  
Soohyun Lee ◽  
Suwon Jeon ◽  
Goon-Tae Kim ◽  
Eun Jig Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cre Recombinase ◽  
Fusion Tag ◽  
Terminal Poly

Hirudin as a novel fusion tag for efficient production of lunasin in Escherichia coli

2017 ◽  
Vol 47 (6) ◽  
pp. 619-626 ◽  
Author(s):  
Qinghua Tian# ◽  
Ping Zhang# ◽  
Zhan Gao ◽  
Hengli Li ◽  
Zhengli Bai ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Efficient Production ◽  
Fusion Tag

scholarly journals Using Haloarcula marismortui Bacteriorhodopsin as a Fusion Tag for Enhancing and Visible Expression of Integral Membrane Proteins in Escherichia coli

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e56363 ◽  
Author(s):  
Min-Feng Hsu ◽  
Tsung-Fu Yu ◽  
Chia-Cheng Chou ◽  
Hsu-Yuan Fu ◽  
Chii-Shen Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Proteins ◽  
Integral Membrane Proteins ◽  
Haloarcula Marismortui ◽  
Fusion Tag

scholarly journals Ubiquitin-like prokaryotic MoaD as a fusion tag for expression of heterologous proteins in Escherichia coli

2014 ◽  
Vol 14 (1) ◽  
pp. 5 ◽  
Author(s):  
Sujuan Yuan ◽  
Xin Wang ◽  
Jian Xu ◽  
Zheng Yan ◽  
Nan Wang
Keyword(s):  
Escherichia Coli ◽  
Heterologous Proteins ◽  
Fusion Tag

scholarly journals Purification, and activity of Human rhinovirus 3C protease fused with N-terminal GST-tag and C-terminal His-tag (GST-HRV3C-His) expressed in Escherichia coli

2019 ◽  
Vol 16 (3) ◽  
pp. 162
Author(s):  
Le Duong Vuong ◽  
Le Thi Tuong Vy ◽  
Phan Thi Phuong Trang ◽  
Nguyen Duc Hoang
Keyword(s):  
Escherichia Coli ◽  
Specific Activity ◽  
Human Rhinovirus ◽  
Purification Process ◽  
Recognition Sequence ◽  
Fusion Tag ◽  
E Coli ◽  
Specific Recognition ◽  
3C Protease ◽  
His Tag

The Human rhinovirus 3C protease (HRV3C) is one of the most effective enzymes for removing fusion tag in purification process. This protease is often produced as fusion form GST-HRV3C but there is no study about the fusion form: GST-HRV3C-His. In this study, researchers conducted the purification GST-HRV3C-His expressed in E. coli, checked the activity and investigated its application. GST-HRV3C-His could be purified using His-tag column with 86.6% purity and GST column with 96.87%. The specific activity of GST-HRV3C-His was demonstrated to be about 4500 U/mg and its application in the purification of another proteins carrying HRV3C-specific recognition sequence, LEVLFQ¯GP based on His-tag or GST-tag was also proved in this study.

The NT11, a novel fusion tag for enhancing protein expression in Escherichia coli

2019 ◽  
Vol 103 (5) ◽  
pp. 2205-2216 ◽  
Author(s):  
Thi Khoa My Nguyen ◽  
Mi Ran Ki ◽  
Ryeo Gang Son ◽  
Seung Pil Pack
Keyword(s):  
Escherichia Coli ◽  
Protein Expression ◽  
Fusion Tag ◽  
Expression In Escherichia Coli
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