Isolation and characterization of bioactive metabolites from Xylaria psidii, an endophytic fungus of the medicinal plant Aegle marmelos and their role in mitochondrial dependent apoptosis against pancreatic cancer cells

Phytomedicine ◽  
2016 ◽  
Vol 23 (12) ◽  
pp. 1312-1320 ◽  
Author(s):  
Divya Arora ◽  
Nisha Sharma ◽  
Venugopal Singamaneni ◽  
Vishal Sharma ◽  
Manoj Kushwaha ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Medicinal Plant ◽  
Cancer Cells ◽  
Endophytic Fungus ◽  
Bioactive Metabolites ◽  
Aegle Marmelos ◽  
Pancreatic Cancer Cells ◽  
Isolation And Characterization

Galectin-3 is modulated in pancreatic cancer cells under hypoxia and nutrient deprivation

2020 ◽  
Vol 401 (10) ◽  
pp. 1153-1165 ◽  
Author(s):  
Antônio F. da Silva Filho ◽  
Lucas B. Tavares ◽  
Maira G. R. Pitta ◽  
Eduardo I. C. Beltrão ◽  
Moacyr J. B. M. Rêgo
Keyword(s):  
Pancreatic Cancer ◽  
Cell Death ◽  
Mrna Expression ◽  
Cancer Cells ◽  
Ductal Adenocarcinoma ◽  
Reverse Transcription Pcr ◽  
Pancreatic Cancer Cells ◽  
Through Flow ◽  
Galectin 3

AbstractPancreatic ductal adenocarcinoma is one of the most aggressive tumors with a microenvironment marked by hypoxia and starvation. Galectin-3 has been evaluated in solid tumors and seems to present both pro/anti-tumor effects. So, this study aims to characterize the expression of Galectin-3 from pancreatic tumor cells and analyze its influence for cell survive and motility in mimetic microenvironment. For this, cell cycle and cell death were accessed through flow cytometry. Characterization of inside and outside Galectin-3 was performed through Real-Time Quantitative Reverse Transcription PCR (qRT-PCR), immunofluorescence, Western blot, and ELISA. Consequences of Galectin-3 extracellular inhibition were investigated using cell death and scratch assays. PANC-1 showed increased Galectin-3 mRNA expression when cultivated in hypoxia for 24 and 48 h. After 24 h in simultaneously hypoxic/deprived incubation, PANC-1 shows increased Galectin-3 protein and secreted levels. For Mia PaCa-2, cultivation in deprivation was determinant for the increasing in Galectin-3 mRNA expression. When cultivated in simultaneously hypoxic/deprived condition, Mia PaCa-2 also presented increasing for the Galectin-3 secreted levels. Treatment of PANC-1 cells with lactose increased the death rate when cells were incubated simultaneously hypoxic/deprived condition. Therefore, it is possible to conclude that the microenvironmental conditions modulate the Galectin-3 expression on the transcriptional and translational levels for pancreatic cancer cells.

SPROUTY2 EXPRESSION IN HUMAN PANCREATIC CANCER CELLS AND CHARACTERIZATION OF THE SPROUTY2 PROMOTER.

Pancreas ◽  
2004 ◽  
Vol 29 (4) ◽  
pp. 335-336
Author(s):  
C.Y. Chan ◽  
U. Burley ◽  
X.Z. Ding ◽  
M.S. Talamonti ◽  
R.H. Bell ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Human Pancreatic Cancer ◽  
Pancreatic Cancer Cells

Characterization of the Bystander Effect of Somatostatin Receptor sst2 After In Vivo Gene Transfer into Human Pancreatic Cancer Cells

2005 ◽  
Vol 16 (10) ◽  
pp. 1175-1193 ◽  
Author(s):  
Nicolas Carrere ◽  
Fabienne Vernejoul ◽  
Anny Souque ◽  
Amani Asnacios ◽  
Nicole Vaysse ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Gene Transfer ◽  
Cancer Cells ◽  
Bystander Effect ◽  
Somatostatin Receptor ◽  
Human Pancreatic Cancer ◽  
Pancreatic Cancer Cells ◽  
In Vivo Gene Transfer

Characterization of BAG3 cleavage during apoptosis of pancreatic cancer cells

2010 ◽  
pp. n/a-n/a ◽  
Author(s):  
Hua-Qin Wang ◽  
Xin Meng ◽  
Yan-Yan Gao ◽  
Bao-Qin Liu ◽  
Xiao-Fang Niu ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Pancreatic Cancer Cells

scholarly journals An Overview of Methods of Extraction, Isolation and Characterization of Natural Medicinal Plant Products in Improved Traditional Medicine Research

2020 ◽  
pp. 31-57
Author(s):  
Dobgima John Fonmboh ◽  
Ejoh Richard Abah ◽  
Tembe Estella Fokunang ◽  
Bayaga Herve ◽  
Gerald Ngo Teke ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Traditional Medicine ◽  
Chemical Diversity ◽  
Bioactive Metabolites ◽  
Plant Biodiversity ◽  
Phytochemical Screening ◽  
Herbal Products ◽  
Isolation And Characterization ◽  
Herbal Plants

Herbal plants are very important in traditional community use and enrich our plant biodiversity and conservation. Natural products are vital substances of traditional knowledge systems in complementary and alternative medicine, nutraceutical, food supplements, and pharmaceutical bioactive metabolites of new chemical entities. Bioactive secondary metabolites from herbal plants of different forms are main sources and provide major opportunities for drug active pharmaceuticals due to the diverse flora and fauna biodiversity that produces the necessary available chemical diversity. There has been an increasing popularity in phytochemical research within the high through put (HTS) screening programs in search of lead. Phytochemicals of herbal extracts for traditional uses contain various types of bioactive metabolites of pharmaceutical and pharmacotherapeutic nature, and many phytomedicines for different therapeutic areas have been derived from herbal products. This paper is aimed at giving an insight into the extraction, isolation, and characterization of the rich medicinal plant biodiversity of potential pharmaceutical importance and the major drawbacks and challenges in the extraction, isolation, and characterization of phytochemicals in plant extracts. Phytochemicals in medicinal plants have been studied with more emphasis on the extraction process which is a vital stage in the analysis of bioactive compounds in medicinal plant research. The advantages and disadvantages of the different extraction methods is important to discuss following the regulatory guidelines and different pharmacopoeia. The analysis of bioactive molecules in herbal products involves the applications of various phytochemical screening methods, and the use of chromatographic techniques such as TLC and HPLC, including in some cases the non-chromatographic methods like Fourier Transform Infra-Red (FTIR), immunoassay. This paper has been motivated by the challenges faced by most pharmacy students in data mining of information on phytochemical screening and testing of biological activities in projects related to herbal plants research. This write up is also geared towards providing students with information on the preclinical drug discovery process towards the formulation of an improved traditional medicine/ phytomedicine.

Abstract 1902: Characterization of choline phospholipid metabolism in pancreatic cancer cells.

2013 ◽  
Author(s):  
Tariq Shah ◽  
Flonne Wildes ◽  
Yelena Mironchik ◽  
Marie-France Penet ◽  
Anirban Maitra ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Phospholipid Metabolism ◽  
Pancreatic Cancer Cells ◽  
Choline Phospholipid
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