Antisense-mediated reduction in ADC activity causes minor alterations in the alkaloid profile of cultured hairy roots and regenerated transgenic plants of Nicotiana tabacum

2007 ◽  
Vol 68 (19) ◽  
pp. 2465-2479 ◽  
Author(s):  
Yupyn Chintapakorn ◽  
John D. Hamill
Keyword(s):  
Nicotiana Tabacum ◽  
Transgenic Plants ◽  
Hairy Roots ◽  
Mediated Reduction

Antioxidant response of tobacco (Nicotiana tabacum) hairy roots after phenol treatment

2011 ◽  
Vol 49 (9) ◽  
pp. 1020-1028 ◽  
Author(s):  
Lucas G. Sosa Alderete ◽  
Elizabeth Agostini ◽  
María I. Medina
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots ◽  
Antioxidant Response ◽  
Phenol Treatment

scholarly journals Identification of a Novel NtLRR-RLK and Biological Pathways That Contribute to Tolerance of TMV in Nicotiana tabacum

2020 ◽  
Vol 33 (7) ◽  
pp. 996-1006
Author(s):  
Jing Wang ◽  
Fengsheng Hao ◽  
Kunfeng Song ◽  
Weihuan Jin ◽  
Bo Fu ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Transgenic Plants ◽  
Protein Interaction ◽  
Resistance Genes ◽  
Fluorescent Protein ◽  
Infection Process ◽  
Biological Pathways ◽  
Mass Spectrometry Analysis ◽  
Signal Pathways ◽  
Interaction Map

Tobacco mosaic virus (TMV) infection can causes serious damage to tobacco crops. To explore the approach of preventing TMV infection of plants, two tobacco cultivars with different resistances to TMV were used to analyze transcription profiling before and after TMV infection. The involvement of biological pathways differed between the tolerant variety (Yuyan8) and the susceptible variety (NC89). In particular, the plant–virus interaction pathway was rapidly activated in Yuyan8, and specific resistance genes were enriched. Liquid chromatography tandem mass spectrometry analysis detected large quantities of antiviral substances in the tolerant Yuyan8. A novel Nicotiana tabacum leucine-rich repeat receptor kinase (NtLRR-RLK) gene was identified as being methylated and this was verified using bisulfite sequencing. Transient expression of TMV-green fluorescent protein in pRNAi-NtLRR-RLK transgenic plants confirmed that NtLRR-RLK was important for susceptibility to TMV. The specific protein interaction map generated from our study revealed that levels of BIP1, E3 ubiquitin ligase, and LRR-RLK were significantly elevated, and all were represented at node positions in the protein interaction map. The same expression tendency of these proteins was also found in pRNAi-NtLRR-RLK transgenic plants at 24 h after TMV inoculation. These data suggested that specific genes in the infection process can activate the immune signal cascade through different resistance genes, and the integration of signal pathways could produce resistance to the virus. These results contribute to the overall understanding of the molecular basis of plant resistance to TMV and in the long term could identify new strategies for prevention and control virus infection.

scholarly journals Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6β-hydroxylase

2005 ◽  
Vol 56 (420) ◽  
pp. 2611-2618 ◽  
Author(s):  
Suvi T. Häkkinen ◽  
Elisabeth Moyano ◽  
Rosa M. Cusidó ◽  
Javier Palazón ◽  
M. Teresa Piñol ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots ◽  
Tropane Alkaloids ◽  
Enhanced Secretion

scholarly journals The MAR-Mediated Reduction in Position Effect Can Be Uncoupled from Copy Number-Dependent Expression in Transgenic Plants.

1995 ◽  
pp. 599-609 ◽  
Author(s):  
L. Mlynarova ◽  
R. C. Jansen ◽  
A. J. Conner ◽  
W. J. Stiekema ◽  
J. P. Nap
Keyword(s):  
Transgenic Plants ◽  
Copy Number ◽  
Position Effect ◽  
Mediated Reduction

scholarly journals Expression of the antibody 14D9 in Nicotiana tabacum hairy roots

2005 ◽  
Vol 8 (2) ◽  
pp. 170-176 ◽  
Author(s):  
Carolina Martinez ◽  
Silvana Petruccelli ◽  
Ana Maria Giulietti ◽  
Maria Alejandra Alvarez
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots

scholarly journals An amphibian antimicrobial peptide variant expressed in Nicotiana tabacum confers resistance to phytopathogens1

2003 ◽  
Vol 370 (1) ◽  
pp. 121-127 ◽  
Author(s):  
Donatella PONTI ◽  
M. LUISA MANGONI ◽  
Giuseppina MIGNOGNA ◽  
Maurizio SIMMACO ◽  
Donatella BARRA
Keyword(s):  
Nicotiana Tabacum ◽  
Transgenic Plants ◽  
Antimicrobial Peptide ◽  
Plant Pathogens ◽  
Rana Esculenta ◽  
Cauliflower Mosaic Virus ◽  
35S Promoter ◽  
Skin Secretions ◽  
Fungal Phytopathogens ◽  
Micro Organisms

Esculentin-1 is a 46-residue antimicrobial peptide present in skin secretions of Rana esculenta. It is effective against a wide variety of micro-organisms, including plant pathogens with negligible effects on eukaryotic cells. As a possible approach to enhance plant resistance, a DNA coding for esculentin-1, with the substitution Met-28Leu, was fused at the C-terminal end of the leader sequence of endopolygalacturonase-inhibiting protein, under the control of the cauliflower mosaic virus 35S promoter region, and introduced into Nicotiana tabacum. The antimicrobial peptide was isolated from the intercellular fluids of healthy leaves of transgenic plants, suggesting that it was properly processed, secreted outside cells and accumulated in the intercellular spaces. The morphology of transgenic plants was unaffected. Challenging these plants with bacterial or fungal phytopathogens demonstrated enhanced resistance up to the second generation. Moreover, transgenic plants displayed insecticidal properties.

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