Induced pluripotent stem cells (iPS cell) differentiates to trophoblast

SummaryRecent advances in stem cell biology have transformed the understanding of cell physiology and developmental biology such that it can now play a more prominent role in the clinical application of stem cell and regenerative medicine. Success in the generation of human induced pluripotent stem cells (iPS) as well as related emerging technology on the iPS platform provide great promise in the development of regenerative medicine. Human iPS cells show almost identical properties to human embryonic stem cells (ESC) in pluripotency, but avoid many of their limitations of use. In addition, investigations into reprogramming of somatic cells to pluripotent stem cells facilitate a deeper understanding of human stem cell biology. The iPS cell technology has offered a unique platform for studying the pathogenesis of human disease, pharmacological and toxicological testing, and cell-based therapy. Nevertheless, significant challenges remain to be overcome before the promise of human iPS cell technology can be realised.

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Retention of Human-Induced Pluripotent Stem Cells (hiPS) With Injectable HA Hydrogels for Vocal Fold Engineering

Annals of Otology Rhinology & Laryngology ◽

10.1177/0003489417691296 ◽

2017 ◽

Vol 126 (4) ◽

pp. 304-314 ◽

Cited By ~ 6

Author(s):

Mitsuyoshi Imaizumi ◽

Nicole Y.K. Li-Jessen ◽

Yuka Sato ◽

David T. Yang ◽

Susan L. Thibeault

Keyword(s):

Stem Cells ◽

Hyaluronic Acid ◽

Induced Pluripotent Stem Cells ◽

Pluripotent Stem Cells ◽

Vocal Fold ◽

Vocal Folds ◽

Fish Analysis ◽

Hydrogel Scaffold ◽

Ips Cell ◽

Induced Pluripotent

Objective: One prospective treatment option for vocal fold scarring is regeneration with an engineered scaffold containing induced pluripotent stem cells (iPS). In the present study, we investigated the feasibility of utilizing an injectable hyaluronic acid (HA) scaffold encapsulated with human-iPS cell (hiPS) for regeneration of vocal folds. Methods: Thirty athymic nude rats underwent unilateral vocal fold injury. Contralateral vocal folds served as uninjured controls. Hyaluronic acid hydrogel scaffold, HA hydrogel scaffold containing hiPS, and HA hydrogel scaffold containing hiPS with epidermal growth factor (EGF) were injected in both vocal folds immediately after surgery. One and 2 weeks after injection, larynges were excised for histology, immunohistochemistry, and fluorescence in situ hybridization (FISH). Results: Presence of HA hydrogel was confirmed in vocal folds 1 and 2 weeks post injection. The FISH analysis confirmed the presence and viability of hiPS in the injected vocal folds. Histological results demonstrated that vocal folds injected with HA hydrogel scaffold containing EGF demonstrated less fibrosis than those with HA hydrogel only. Conclusions: Human-iPS survived in injured rat vocal folds. The HA hydrogel with hiPS and EGF ameliorated the fibrotic response. Additional work is necessary to optimize hiPS differentiation and further confirm the safety of hiPS for clinical applications.

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Human induced pluripotent stem cells (iPS cell) differentiate into trophoblast lineage

Placenta ◽

10.1016/j.placenta.2016.08.075 ◽

2016 ◽

Vol 46 ◽

pp. 121

Author(s):

Junya Kojima ◽

Hidenori Akutsu ◽

Naoaki Kuji ◽

Keiichi Isaka

Keyword(s):

Stem Cells ◽

Induced Pluripotent Stem Cells ◽

Pluripotent Stem Cells ◽

Ips Cell ◽

Induced Pluripotent

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Human induced pluripotent stem cells (iPS cell) differentiate to trophoblast lineage in xeno free cluture

Placenta ◽

10.1016/j.placenta.2016.06.227 ◽

2016 ◽

Vol 45 ◽

pp. 127

Author(s):

Junya Kojima ◽

Hidenori Akutsu ◽

Hirotaka Nishi ◽

Naoaki Kuji ◽

Keiichi Isaka

Keyword(s):

Stem Cells ◽

Induced Pluripotent Stem Cells ◽

Pluripotent Stem Cells ◽

Ips Cell ◽

Induced Pluripotent

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Nonhuman Primate Induced Pluripotent Stem Cells in Regenerative Medicine

Stem Cells International ◽

10.1155/2012/767195 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 15

Author(s):

Yuehong Wu ◽

Anuja Mishra ◽

Zhifang Qiu ◽

Steven Farnsworth ◽

Suzette D. Tardif ◽

...

Keyword(s):

Stem Cells ◽

Regenerative Medicine ◽

Induced Pluripotent Stem Cells ◽

Cell Lines ◽

Pluripotent Stem Cells ◽

Patient Specific ◽

Ips Cell ◽

Disease States ◽

Autologous Cell Therapy ◽

Induced Pluripotent

Among the various species from which induced pluripotent stem cells have been derived, nonhuman primates (NHPs) have a unique role as preclinical models. Their relatedness to humans and similar physiology, including central nervous system, make them ideal for translational studies. We review here the progress made in deriving and characterizing iPS cell lines from different NHP species. We focus on iPS cell lines from the marmoset, a small NHP in which several human disease states can be modeled. The marmoset can serve as a model for the implementation of patient-specific autologous cell therapy in regenerative medicine.

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Induced Pluripotent Stem Cells: Inventors turning into competitors

Journal of Commercial Biotechnology ◽

10.5912/jcb756 ◽

2017 ◽

Vol 22 (4) ◽

Author(s):

Pratap Devarapalli ◽

Swapnil Laxman Bhalke ◽

Neelam Sudhir Dharmadhikari ◽

Vandana Mishra ◽

Neha Mago ◽

...

Keyword(s):

Stem Cells ◽

Induced Pluripotent Stem Cells ◽

Pluripotent Stem Cells ◽

Large Scale ◽

Embryonic Stem ◽

Cell Types ◽

Cellular Dynamics ◽

Ips Cell ◽

Induced Pluripotent ◽

James Thomson

Induced Pluripotent Stem Cells (IPSCs) are a kind of adult cells that have been genetically reprogrammed to become different cell types. Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem cells. IPSCs technology was pioneered by Shinya Yamanaka from Kyoto University. This breakthrough has inspired researchers to start working around IPSC technology. James Thomson from University of California has developed IPS cell lines derived from Human Somatic Cells. Subsequently, he also established a large scale human IPSC manufacturing company named Cellular Dynamics International. Thus, increasing interest in the commercial exploitation of IPSCs patents has lead us to look into the patent portfolios of top three patent assignees in IPSC technology. In this study, we have discussed technological patent trends and multiple factors which reflect the competitive scenario between the top assignees of IPSC technology. Our conclusions suggest that Kyoto University led by inventor Shinya Yamanaka is the leader of IPSC technology. However, patent-product linkage analysis suggests that Cellular Dynamics International led by inventor James Thomson may surpass Kyoto University in near future.

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