Analysis of two heterologous flowering genes in Brachypodium distachyon demonstrates its potential as a grass model plant

Stomata are cellular pores on the leaf epidermis that allow plants to regulate carbon assimilation and water loss. Stomata integrate environmental signals to regulate pore apertures and optimize gas exchange to fluctuating conditions. Here, we quantified intraspecific plasticity of stomatal gas exchange and anatomy in response to seasonal variation in Brachypodium distachyon. Over the course of two years we (i) used infrared gas analysis to assess light response kinetics of 120 Bd21-3 wild-type individuals in an environmentally fluctuating greenhouse and (ii) microscopically determined the seasonal variability of stomatal anatomy in a subset of these plants. We observed systemic environmental effects on gas exchange measurements and remarkable intraspecific plasticity of stomatal anatomical traits. To reliably link anatomical variation to gas exchange, we adjusted anatomical gsmax calculations for grass stomatal morphology. We propose that systemic effects and variability in stomatal anatomy should be accounted for in long-term gas exchange studies.

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Evaluation of metabolite extraction protocols and determination ofphysiological response to drought stress via reporter metabolites in model plant Brachypodium distachyon

TURKISH JOURNAL OF BOTANY ◽

10.3906/bot-1505-28 ◽

2015 ◽

Vol 39 ◽

pp. 1042-1050 ◽

Cited By ~ 1

Author(s):

Özge TATLI ◽

Emrah NİKEREL ◽

Bahar SOĞUTMAZ ÖZDEMİR

Keyword(s):

Drought Stress ◽

Brachypodium Distachyon ◽

Model Plant ◽

Metabolite Extraction

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A DNA Barcoding Method to Discriminate between the Model Plant Brachypodium distachyon and Its Close Relatives B. stacei and B. hybridum (Poaceae)

PLoS ONE ◽

10.1371/journal.pone.0051058 ◽

2012 ◽

Vol 7 (12) ◽

pp. e51058 ◽

Cited By ~ 46

Author(s):

Diana López-Alvarez ◽

Maria Luisa López-Herranz ◽

Alexander Betekhtin ◽

Pilar Catalán

Keyword(s):

Dna Barcoding ◽

Brachypodium Distachyon ◽

Model Plant ◽

Close Relatives

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Anatomy of epithemal hydathodes in four monocotyledon plants of economic and academic relevance

10.1101/2020.04.20.050823 ◽

2020 ◽

Author(s):

Alain Jauneau ◽

Aude Cerutti ◽

Marie-Christine Auriac ◽

Laurent D. Noël

Keyword(s):

Microscopic Examination ◽

Vascular Plants ◽

Brachypodium Distachyon ◽

External Environment ◽

Leaf Margin ◽

Entry Site ◽

Future Studies ◽

Model Plant ◽

Plant Organ ◽

Vascular Pathogens

AbstractHydathode is a plant organ responsible for guttation in vascular plants, i.e. the release of droplets at leaf margin or surface. Because this organ connects the plant vasculature to the external environment, it is also a known entry site for some vascular pathogens. In this study, we present a detailed microscopic examination of monocot hydathodes for three crops (maize, rice and sugarcane) and the model plant Brachypodium distachyon. Our study highlights both similarities and specificities of those epithemal hydathodes. These observations will serve as a foundation for future studies on the physiology and the immunity of hydathodes in monocots.

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Identification and Characterization of NF-Y Transcription Factor Families in the Monocot Model Plant Brachypodium distachyon

PLoS ONE ◽

10.1371/journal.pone.0021805 ◽

2011 ◽

Vol 6 (6) ◽

pp. e21805 ◽

Cited By ~ 55

Author(s):

Shuanghe Cao ◽

Roderick W. Kumimoto ◽

Chamindika L. Siriwardana ◽

Jan R. Risinger ◽

Ben F. Holt

Keyword(s):

Transcription Factor ◽

Brachypodium Distachyon ◽

Model Plant ◽

Identification And Characterization

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Discovery of a Xylem Discontinuity in the Model Plant Brachypodium distachyon

Microscopy and Microanalysis ◽

10.1017/s1431927612002620 ◽

2012 ◽

Vol 18 (S2) ◽

pp. 154-155

Author(s):

A.J. Bowling ◽

O.P. Zhivotovsky-Castello

Keyword(s):

Brachypodium Distachyon ◽

Model Plant

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.

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Transcriptional regulation of ZIP genes is independent of local zinc status in Brachypodium shoots upon zinc deficiency and resupply

10.1101/2020.11.23.393827 ◽

2020 ◽

Author(s):

Sahand Amini ◽

Borjana Arsova ◽

Sylvie Gobert ◽

Monique Carnol ◽

Bernard Bosman ◽

...

Keyword(s):

Zinc Deficiency ◽

Brachypodium Distachyon ◽

Zinc Homeostasis ◽

Plant Responses ◽

Rna Seq ◽

Molecular Events ◽

Whole Plant ◽

Grass Model ◽

Flux Measurements ◽

Zip Genes

AbstractThe biological processes underlying zinc homeostasis are targets for genetic improvement of crops to counter human malnutrition. Detailed phenotyping, ionomic, RNA-Seq analyses and flux measurements with 67Zn isotope revealed whole plant molecular events underlying zinc homeostasis upon varying zinc supply and during zinc resupply to starved Brachypodium distachyon (Brachypodium) plants. Although both zinc deficiency and excess hindered Brachypodium growth, accumulation of biomass and micronutrients into roots and shoots differed depending on zinc supply. The zinc resupply dynamics involved 1893 zinc-responsive genes. Multiple ZIP transporter genes and dozens of other genes were rapidly and transiently down-regulated in early stages of zinc resupply, suggesting a transient zinc shock, sensed locally in roots. Notably genes with identical regulation were observed in shoots without zinc accumulation, pointing to root-to-shoot signals mediating whole plant responses to zinc resupply. Molecular events uncovered in the grass model Brachypodium are useful for the improvement of staple monocots.

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