Constitutive expression of DaCBF7, an Antarctic vascular plant Deschampsia antarctica CBF homolog, resulted in improved cold tolerance in transgenic rice plants

The Antarctic flowering plant Deschampsia antarctica is highly sensitive to climate change and has shown rapid population increases during regional warming of the Antarctic Peninsula. Several studies have examined the physiological and biochemical changes related to environmental stress tolerance that allow D. antarctica to colonize harsh Antarctic environments; however, the molecular mechanisms of its responses to environmental changes remain poorly understood. To elucidate the survival strategies of D. antarctica in Antarctic environments, we investigated the functions of actin depolymerizing factor (ADF) in this species. We identified eight ADF genes in the transcriptome that were clustered into five subgroups by phylogenetic analysis. DaADF3, which belongs to a monocot-specific clade together with cold-responsive ADF in wheat, showed significant transcriptional induction in response to dehydration and cold, as well as under Antarctic field conditions. Multiple drought and low-temperature responsive elements were identified as possible binding sites of C-repeat-binding factors in the promoter region of DaADF3, indicating a close relationship between DaADF3 transcription control and abiotic stress responses. To investigate the functions of DaADF3 related to abiotic stresses in vivo, we generated transgenic rice plants overexpressing DaADF3. These transgenic plants showed greater tolerance to low-temperature stress than the wild-type in terms of survival rate, leaf chlorophyll content, and electrolyte leakage, accompanied by changes in actin filament organization in the root tips. Together, our results imply that DaADF3 played an important role in the enhancement of cold tolerance in transgenic rice plants and in the adaptation of D. antarctica to its extreme environment.

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Native polyubiquitin promoter of rice provides increased constitutive expression in stable transgenic rice plants

Plant Cell Reports ◽

10.1007/s00299-011-1161-4 ◽

2011 ◽

Vol 31 (2) ◽

pp. 271-279 ◽

Cited By ~ 20

Author(s):

Jagannath Bhattacharyya ◽

Asif Hasan Chowdhury ◽

Samarjit Ray ◽

Jyoti Krishna Jha ◽

Srirupa Das ◽

...

Keyword(s):

Transgenic Rice ◽

Constitutive Expression ◽

Rice Plants ◽

Transgenic Rice Plants

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Constitutive expression of pathogen-inducible OsWRKY31 enhances disease resistance and affects root growth and auxin response in transgenic rice plants

Cell Research ◽

10.1038/cr.2007.104 ◽

2007 ◽

Vol 18 (4) ◽

pp. 508-521 ◽

Cited By ~ 124

Author(s):

Juan Zhang ◽

Youliang Peng ◽

Zejian Guo

Keyword(s):

Disease Resistance ◽

Root Growth ◽

Transgenic Rice ◽

Constitutive Expression ◽

Auxin Response ◽

Rice Plants ◽

Transgenic Rice Plants

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Constitutive expression of CaPLA1 conferred enhanced growth and grain yield in transgenic rice plants

Plant Molecular Biology ◽

10.1007/s11103-016-0440-4 ◽

2016 ◽

Vol 90 (4-5) ◽

pp. 517-532 ◽

Cited By ~ 3

Author(s):

Ki Youl Park ◽

Eun Yu Kim ◽

Young Sam Seo ◽

Woo Taek Kim

Keyword(s):

Grain Yield ◽

Transgenic Rice ◽

Constitutive Expression ◽

Rice Plants ◽

Transgenic Rice Plants

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Poaceae Type II Galactinol Synthase 2 from Antarctic Flowering Plant Deschampsia antarctica and Rice Improves Cold and Drought Tolerance by Accumulation of Raffinose Family Oligosaccharides in Transgenic Rice Plants

Plant and Cell Physiology ◽

10.1093/pcp/pcz180 ◽

2019 ◽

Vol 61 (1) ◽

pp. 88-104 ◽

Cited By ~ 4

Author(s):

Li Hua Cui ◽

Mi Young Byun ◽

Hyeong Geun Oh ◽

Sung Jin Kim ◽

Jungeun Lee ◽

...

Keyword(s):

Transgenic Rice ◽

Flowering Plant ◽

Deschampsia Antarctica ◽

Type Ii ◽

Wild Type ◽

Raffinose Family Oligosaccharides ◽

Galactinol Synthase ◽

Rice Plants ◽

Transgenic Rice Plants ◽

In The Wild

Abstract Deschampsia antarctica is a Poaceae grass that has adapted to and colonized Antarctica. When D. antarctica plants were subjected to cold and dehydration stress both in the Antarctic field and in laboratory experiments, galactinol, a precursor of raffinose family oligosaccharides (RFOs) and raffinose were highly accumulated, which was accompanied by upregulation of galactinol synthase (GolS). The Poaceae monocots have a small family of GolS genes, which are divided into two distinct groups called types I and II. Type II GolSs are highly expanded in cold-adapted monocot plants. Transgenic rice plants, in which type II D. antarctica GolS2 (DaGolS2) and rice GolS2 (OsGolS2) were constitutively expressed, were markedly tolerant to cold and drought stress as compared to the wild-type rice plants. The RFO contents and GolS enzyme activities were higher in the DaGolS2- and OsGolS2-overexpressing progeny than in the wild-type plants under both normal and stress conditions. DaGolS2 and OsGolS2 overexpressors contained reduced levels of reactive oxygen species (ROS) relative to the wild-type plants after cold and drought treatments. Overall, these results suggest that Poaceae type II GolS2s play a conserved role in D. antarctica and rice in response to drought and cold stress by inducing the accumulation of RFO and decreasing ROS levels.

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Constitutive expression of human lactoferrin and its N-lobe in rice plants to confer disease resistance

Biochemistry and Cell Biology ◽

10.1139/o05-022 ◽

2005 ◽

Vol 83 (2) ◽

pp. 239-249 ◽

Cited By ~ 15

Author(s):

Kenji Takase ◽

Kiyoshi Hagiwara ◽

Haruko Onodera ◽

Yaeko Nishizawa ◽

Masashi Ugaki ◽

...

Keyword(s):

Disease Resistance ◽

Transgenic Rice ◽

Plant Disease Resistance ◽

Constitutive Expression ◽

Cauliflower Mosaic Virus ◽

Human Lactoferrin ◽

Binding Property ◽

Heparin Binding ◽

Rice Plants ◽

Transgenic Rice Plants

The milk protein, lactoferrin, is known to have antibacterial, antiviral, and antifungal activities. To explore the possibility of conferring disease resistance in plants by expressing this protein, the gene for the full-length human lactoferrin (HLF), as well as the N-lobe, the N-terminal half molecule (HLFN), was introduced into rice plants and expressed constitutively under the control of the cauliflower mosaic virus 35S promotor. Western blot analysis of leaves from HLF-transgenic rice plants showed an 80 kDa-band, which was about 1–2 kDa less than human milk lactoferrin. HLFN was expressed as a 45-kDa protein and retained its heparin-binding property. Deglycosylation experiments suggested that both proteins produced by the plants had plant-type oligosaccharide chains. The transgenic rice plants were assessed for resistance against disease-causing bacteria, virus, and fungi. Of the pathogens tested, significant resistance against Burkholderia (Pseudomonas) plantarii, the causative agent of bacterial seedling blight disease, was observed in the transgenic plants expressing HLF or HLFN.Key words: expression of domains; glycosylation; lactoferrin; plant disease resistance; transgenic rice.

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