Overexpression of four Arabidopsis thaliana NHL genes in soybean (Glycine max) roots and their effect on resistance to the soybean cyst nematode (Heterodera glycines)

2014 ◽  
Vol 86 ◽  
pp. 1-10 ◽  
Author(s):  
Andrea Maldonado ◽  
Reham Youssef ◽  
Margaret McDonald ◽  
Eric Brewer ◽  
Hunter Beard ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Glycine Max ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode

AAC Richard Soybean

2022 ◽  
Author(s):  
Kangfu Yu ◽  
Lorna Woodrow ◽  
M. Chun Shi
Keyword(s):  
Glycine Max ◽  
Research And Development ◽  
Soybean Cyst Nematode ◽  
Protein Concentration ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Processing Quality ◽  
Food Grade ◽  
High Protein Concentration ◽  
Glycine Max L

AAC Richard is a food grade soybean [Glycine max (L.) Merr] cultivar with yellow hilum, high protein concentration, and good processing quality for foreign and domestic soymilk, tofu, and miso markets. It has resistance to SCN (soybean cyst nematode) (Heterodera Glycines Ichinohe). AAC Richard was developed at the Agriculture and Agri-Food Canada (AAFC) Harrow Research and Development Centre (Harrow-RDC), Harrow, Ontario and is adapted to areas of southwest Ontario with 3100 or more crop heat units and has a relative maturity of 2.3 (MG 2.3).

Localization of hydrogen peroxide during the defence response of Arabidopsis thaliana against the plant-parasitic nematode Heterodera glycines

Nematology ◽  
1999 ◽  
Vol 1 (7) ◽  
pp. 681-686 ◽  
Author(s):  
Georg Waetzig ◽  
Miroslaw Sobczak ◽  
Florian Grundler
Keyword(s):  
Hydrogen Peroxide ◽  
Arabidopsis Thaliana ◽  
Soybean Cyst Nematode ◽  
Parasitic Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Defence Response ◽  
H2o2 Production ◽  
Cerium Chloride ◽  
Feeding Site

AbstractHydrogen peroxide (H2O2) production during the infection of Arabidopsis thaliana by the soybean cyst nematode Heterodera glycines was detected histochemically by the reaction of H2O2 with cerium chloride producing four different patterns of electron-dense precipitates of cerium perhydroxides. As A. thaliana is not a regular host of H. glycines, the defence response is considerable, but does not completely inhibit the development of the nematode. H2O2 was produced not only by cells mechanically damaged during invasion and feeding site induction by the nematode, but also by cells surrounding developing syncytia and cells which were neither in contact with the nematode nor with the syncytium. Die Lokalisation von Peroxid wahrend der Abwehrreaktion von Arabidopsis thaliana gegen den pflanzenparasitaren Nematoden Heterodera glycines - Die Bildung von Wasserstoffperoxid (H2O2) im Rahmen der Infektion von Arabidopsis thaliana durch den Sojabohnen-Zystennematoden Heterodera glycines wurde histochemisch durch die Reaktion von H2O2 mit Cerchlorid nachgewiesen, wobei vier verschiedene Muster elektronendichter Prazipitate von Cerperhydroxiden gebildet wurden. Da A. thaliana kein regularer Wirt von H. glycines ist, kommt es zu einer betrachtlichen Abwehrreaktion, die jedoch die Entwicklung des Nematoden nicht vollstandig verhindert. H2O2 wurde nicht nur von Zellen produziert, die im Laufe des Eindringens und der Induktion des Nahrzellensystems durch den Nematoden mechanisch beschadigt worden waren, sondern auch von Zellen, die sich entwickelnde Syncytien umgaben und von Zellen, die weder mit dem Nematoden noch mit dem Syncytium in Kontakt standen.

Timecourse microarray analyses reveal global changes in gene expression of susceptible Glycine max (soybean) roots during infection by Heterodera glycines (soybean cyst nematode)

Planta ◽  
2006 ◽  
Vol 224 (4) ◽  
pp. 838-852 ◽  
Author(s):  
Nadim W. Alkharouf ◽  
Vincent P. Klink ◽  
Imed B. Chouikha ◽  
Hunter S. Beard ◽  
Margaret H. MacDonald ◽  
...  
Keyword(s):  
Gene Expression ◽  
Glycine Max ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Global Changes ◽  
Soybean Roots ◽  
Microarray Analyses

scholarly journals Known Distribution of the Soybean Cyst Nematode, Heterodera glycines, in the United States and Canada in 2020

2021 ◽  
Author(s):  
Gregory L. Tylka ◽  
Christopher C. Marett
Keyword(s):  
United States ◽  
Glycine Max ◽  
Health Professionals ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
The United States ◽  
Cyst Nematode ◽  
Soil Sampling ◽  
Rural Municipalities ◽  
Canadian Provinces

In the United States and Canada, the most damaging pathogen of soybean, Glycine max, is the soybean cyst nematode (SCN), Heterodera glycines. Plant health professionals working for universities and state and provincial departments of agriculture in the United States and Canada are queried periodically about counties and rural municipalities that are newly known to be infested with SCN in their states and provinces. Such a census was conducted in 2020, and the results were compared with results of the most recent survey, published in 2017. Between 2017 and 2020, 55 new SCN-infested counties were reported from 11 U.S. states. Also, 24 new SCN-infested counties and rural municipalities were identified in the Canadian provinces of Manitoba, Ontario, and Quebec. A map of the known distribution of SCN in these two countries was updated. The results reveal steady expansion of the distribution of SCN throughout the United States and Canada, and the pest almost certainly will continue to spread among and within soybean-producing areas of these countries in the future. Therefore, continued scouting and soil sampling for detection of new SCN infestations are warranted as the first step toward successfully managing the pathogen.

Effects of agronomic practices on the timeline of Heterodera glycines establishment in a new location

Nematology ◽  
2015 ◽  
Vol 17 (6) ◽  
pp. 705-713 ◽  
Author(s):  
Haddish Melakeberhan ◽  
Wei Wang ◽  
Alexandra Kravchenko ◽  
Kurt Thelen
Keyword(s):  
Zea Mays ◽  
Glycine Max ◽  
Population Density ◽  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Sandy Loam ◽  
Cyst Nematode ◽  
Agronomic Practices ◽  
No Till ◽  
New Location

How soybean cyst nematode (SCN,Heterodera glycines) adapts when introduced into a new location under tillage, rotation and crop treatments is unknown. SCN race 3 (Hg Type 0) was introduced into a sandy loam field at more than 4000 eggs (100 cm3soil)−1and observed over 6 years under till and no-till, and either maize (Zea mays; C), SCN race 3 resistant soybean (Glycine max; R) or susceptible soybean (S) monocrop, or RCRC and SCSC rotations. While SCN population density was lower in no-till than in tilled treatments, and highest in S and lowest in C or RC rotations, it was detected at less than 1 cyst (100 cm3soil)−1. This suggests a prolonged phase of decline from the introduced levels. The interaction effects of tillage, rotation and/or time on SCN suggest that outcomes vary by agronomic practice and time, providing agro-biologically-based understanding of SCN establishment in a new location.

RNA-Seq-based identification of potential resistance mechanism against the soybean cyst nematode (Heterodera glycines) HG Type 0 in soybean (Glycine max) cv. Dongnong L-204

2020 ◽  
Vol 71 (6) ◽  
pp. 539
Author(s):  
Haipeng Jiang ◽  
Fanshan Bu ◽  
Lizheng Tian ◽  
Qiuxia Sun ◽  
Dongfang Bao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Glycine Max ◽  
Stress Response ◽  
Soybean Cyst Nematode ◽  
High Throughput Sequencing ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Cell Junction ◽  
Extracellular Region

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is the most important disease affecting yield of soybean (Glycine max (L.) Merr.). In China, among the main physiological races of SCN causing serious economic loss of soybean, HG Type 0 is the most widely distributed. Breeding SCN-resistant varieties is one of the safest and most effective ways to manage SCN. Soybean cv. Dongnong L-204 has a green seed coat and is a resource for resistance to SCN; however, the transcriptional variation and main molecular mechanism of resistance of Dongnong L-204 are not clear. We obtained 66.42 Gb of raw reads by using Illumina HiSeq high-throughput sequencing of Dongnong L-204. Transcriptional changes at Days 3, 7 and 10 after HG Type 0 stress are described. In total, 11279 differentially expressed genes were responsive to HG Type 0 stress, including 6407 SCN-induced and 4872 SCN-suppressed genes. Gene Ontology analysis emphasised the important roles of the terms metabolic process, single-organism process, catalytic activity and binding under HG Type 0 stress. Among them, the terms related to extracellular matrix, extracellular region part, membrane-enclosed lumen, protein-binding transcription factor activity, and symplast and cell junction existed only in pairwise comparisons Day 7 vs Day 0 and Day 10 vs Day 0. KEGG enrichment analysis showed that metabolic pathway played an important role in the stress response to HG Type 0. Cellular processes, metabolism and organismal systems and other pathway-related genes increased significantly after 7 days. Transcription factor analysis showed that transcription factors such as MYB, AP2-EREBP, bHLH, WRKY and NAC may be involved in the anti-HG Type 0 response of Dongnong L-204. At the same time, few transient inducible transcription factors were found and more transient inhibited transcription factors in Dongnong L-204 in the early stage of HG Type 0 syncytial establishment. RT-qPCR was used to analyse expression of 10 genes closely related to the HG Type 0 stress response. The expression of GmWRKYX1 was >60 times higher at Day 10 after HG Type 0 stress than at Day 0. The WRKY transcription-factor family may play an important role in the resistance of Dongnong L-204 to HG Type 0 stress.

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