One-step preparation of magnetically responsive materials from non-magnetic powders

AbstractGiven the growing interest in molecular diagnosis, highly extensive and selective detection of genetic targets from a very limited amount of samples is in high demand. We demonstrated the highly sensitive and multiplexed one-step RT-qPCR platform for RNA analysis using microparticles as individual reactors. Those particles are equipped with a controlled release system of thermo-responsive materials, and are able to capture RNA targets inside. The particle-based assay can successfully quantify multiple target RNAs from only 200 pg of total RNA. The assay can also quantify target RNAs from a single cell with the aid of a pre-concentration process. We carried out 8-plex one-step RT-qPCR using tens of microparticles, which allowed extensive mRNA profiling. The circadian cycles were shown by the multiplex one-step RT-qPCR in human cell and human hair follicles. Reliable 24-plex one-step RT-qPCR was developed using a single operation in a PCR chip without any loss of performance (i.e., selectivity and sensitivity), even from a single hair. Many other disease-related transcripts can be monitored using this versatile platform. It can also be used non–invasively for samples obtained in clinics.

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The Activation Efficiency of Mechanophores Can Be Modulated by Adjacent Polymer Composition

10.26434/chemrxiv.13200155.v1 ◽

2020 ◽

Author(s):

Sourabh Kumar ◽

Tim Stauch

Keyword(s):

Structural Parameters ◽

Polymer Composition ◽

Polymer Backbone ◽

Responsive Materials ◽

Responsive Polymers ◽

Quantum Chemical Methods ◽

Mechanochemical Transformation ◽

Activation Rate ◽

Activation Efficiency ◽

One Step

<div> <div> <div> <p>The activation efficiency of mechanophores in stress-responsive polymers is generally limited by the competing process of unspecific scission in other parts of the polymer chain. Here it is shown that the linker between the mechanophore and the polymer backbone determines the force needed to activate the mechanophore. Using quantum chemical methods, it is demonstrated that the activation forces of three mechanophores (Dewar benzene, benzocyclobutene and gem-dichlorocyclopropane) can be adjusted over a range of almost 300% by modifying the chemical composition of the linker. The results are discussed in terms of changes in electron density, strain distribution and structural parameters during the rupture process. Using these findings it is straightforward to either significantly enhance or reduce the activation rate of mechanophores in stress-responsive materials, depending on the desired use case. The methodology is applied to switch a one-step “gating” of a mechanochemical transformation to a two-step process. </p> </div> </div> </div>

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The Activation Efficiency of Mechanophores Can Be Modulated by Adjacent Polymer Composition

10.26434/chemrxiv.13200155 ◽

2020 ◽

Author(s):

Sourabh Kumar ◽

Tim Stauch

Keyword(s):

Structural Parameters ◽

Polymer Composition ◽

Polymer Backbone ◽

Responsive Materials ◽

Responsive Polymers ◽

Quantum Chemical Methods ◽

Mechanochemical Transformation ◽

Activation Rate ◽

Activation Efficiency ◽

One Step

<div> <div> <div> <p>The activation efficiency of mechanophores in stress-responsive polymers is generally limited by the competing process of unspecific scission in other parts of the polymer chain. Here it is shown that the linker between the mechanophore and the polymer backbone determines the force needed to activate the mechanophore. Using quantum chemical methods, it is demonstrated that the activation forces of three mechanophores (Dewar benzene, benzocyclobutene and gem-dichlorocyclopropane) can be adjusted over a range of almost 300% by modifying the chemical composition of the linker. The results are discussed in terms of changes in electron density, strain distribution and structural parameters during the rupture process. Using these findings it is straightforward to either significantly enhance or reduce the activation rate of mechanophores in stress-responsive materials, depending on the desired use case. The methodology is applied to switch a one-step “gating” of a mechanochemical transformation to a two-step process. </p> </div> </div> </div>

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An Interactive Minicomputer Program for the Indexing and Simulation of Electron Diffraction Patterns

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100092670 ◽

1976 ◽

Vol 34 ◽

pp. 542-543

Author(s):

R.P. Goehner ◽

W.T. Hatfield ◽

Prakash Rao

Keyword(s):

Electron Diffraction ◽

Real Time ◽

Pattern Analysis ◽

Flow Diagram ◽

Hard Copy ◽

Time Analysis ◽

Real Time Analysis ◽

Transmission Electron ◽

One Step ◽

Diffraction Patterns

Computer programs are now available in various laboratories for the indexing and simulation of transmission electron diffraction patterns. Although these programs address themselves to the solution of various aspects of the indexing and simulation process, the ultimate goal is to perform real time diffraction pattern analysis directly off of the imaging screen of the transmission electron microscope. The program to be described in this paper represents one step prior to real time analysis. It involves the combination of two programs, described in an earlier paper(l), into a single program for use on an interactive basis with a minicomputer. In our case, the minicomputer is an INTERDATA 70 equipped with a Tektronix 4010-1 graphical display terminal and hard copy unit.A simplified flow diagram of the combined program, written in Fortran IV, is shown in Figure 1. It consists of two programs INDEX and TEDP which index and simulate electron diffraction patterns respectively. The user has the option of choosing either the indexing or simulating aspects of the combined program.

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Nutrient-regulated gene expression in eukaryotes

Biochemical Society Symposium ◽

10.1042/bss0730085 ◽

2006 ◽

Vol 73 ◽

pp. 85-96 ◽

Cited By ~ 8

Author(s):

Richard J. Reece ◽

Laila Beynon ◽

Stacey Holden ◽

Amanda D. Hughes ◽

Karine Rébora ◽

...

Keyword(s):

Gene Expression ◽

Rna Polymerase ◽

Rna Polymerase Ii ◽

Transcriptional Control ◽

Direct Interaction ◽

Signalling Pathways ◽

A Cell ◽

One Step ◽

Higher Eukaryotes ◽

Regulated Gene Expression

The recognition of changes in environmental conditions, and the ability to adapt to these changes, is essential for the viability of cells. There are numerous well characterized systems by which the presence or absence of an individual metabolite may be recognized by a cell. However, the recognition of a metabolite is just one step in a process that often results in changes in the expression of whole sets of genes required to respond to that metabolite. In higher eukaryotes, the signalling pathway between metabolite recognition and transcriptional control can be complex. Recent evidence from the relatively simple eukaryote yeast suggests that complex signalling pathways may be circumvented through the direct interaction between individual metabolites and regulators of RNA polymerase II-mediated transcription. Biochemical and structural analyses are beginning to unravel these elegant genetic control elements.

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