Transformation of Saussurea involucrata by Agrobacterium rhizogenes: Hairy root induction and syringin production

2005 ◽  
Vol 40 (12) ◽  
pp. 3789-3794 ◽  
Author(s):  
Chun-Xiang Fu ◽  
De-Xiu Zhao ◽  
Xiao-Feng Xue ◽  
Zhi-Ping Jin ◽  
Feng Shan Ma
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Root Induction ◽  
Saussurea Involucrata ◽  
Hairy Root Induction

Technology and Application of Hairy Root Culture in Monocotyledons

2021 ◽  
Vol 07 ◽  
Author(s):  
Chang-Qi Hao ◽  
Shuai-Run Wang ◽  
Yi Wang ◽  
Xin-Yi Hou ◽  
Ya-Xuan Jiang ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Food Crops ◽  
Root Induction ◽  
Hairy Root Induction ◽  
Selection Of

Background: Hairy root culture has been widely used in the production of metabolites in dicotyledons, and a large number of food crops and medicinal plants in monocotyledons need to be developed, but there are many difficulties in the induction of hairy roots in monocotyledons. The purpose of this paper is to introduce the inducing methods, influencing factors and application of hairy roots in monocotyledons, and to promote the development of hairy root system in monocotyledons. Methods: The mechanism of action of Agrobacterium rhizogenes and the current situation of hairy root induction, induction methods and influencing factors of monocotyledons were summarized so as to provide convenience for efficient acquisition of hairy root of monocotyledons. Results: Monocotyledons are not easy to produce phenols, cells are prone to lignification, adverse differentiation and selective response to Agrobacterium rhizogenes strains. It is proposed that before induction, plant varieties and explants should be selected, and different infection strains should be screened. In the process of hairy root induction, exogenous inducers such as acetosyringone can be added. Although these factors can provide some help for the induction of hairy roots in monocotyledons, we still need to pay attention to the disadvantages of monocotyledons from dicotyledons at the cellular level. Conclusion: A large number of food crops and medicinal plants are monocotyledons. Hairy root culture can be used to help the breeding and production of medicinal substances. Therefore, it is necessary to pay attention to the selection of varieties and explants, the selection of Agrobacterium rhizogenes and the addition of acetosyringone in the process of hairy root induction so as to improve the production efficiency and facilitate the development and utilization of monocotyledons.

scholarly journals Effect of Medium Supplements on Agrobacterium rhizogenes Mediated Hairy Root Induction from the Callus Tissues of Camellia sinensis var. sinensis

2016 ◽  
Vol 17 (7) ◽  
pp. 1132 ◽  
Author(s):  
Mohammad Rana ◽  
Zhuo-Xiao Han ◽  
Da-Peng Song ◽  
Guo-Feng Liu ◽  
Da-Xiang Li ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Camellia Sinensis ◽  
Root Induction ◽  
Hairy Root Induction ◽  
Callus Tissues

Evaluation of influence of different strains of Agrobacterium rhizogenes on efficiency of hairy root induction in Rauwolfia serpentina

2020 ◽  
Vol 79 (04) ◽  
Author(s):  
Pratima Bhagat ◽  
Sachin Kumar Verma ◽  
Amit Kumar Singh ◽  
Gajendra Kumar Aseri ◽  
Neeraj Khare
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Transformation Efficiency ◽  
Effective Means ◽  
Root Induction ◽  
Infection Time ◽  
Rauwolfia Serpentina ◽  
Cultivation Period ◽  
Significant Difference ◽  
Hairy Root Induction

An improved protocol was established for hairy root induction in Rauwolfia serpentina using two strains of Agrobacterium rhizogenes MTCC 532 and 2364. Hairy root induction efficiency was standardized based on infection time, co-cultivation period, OD600 and acetosyringone concentration. Higher transformation efficiency was established using MTCC 532 (31%) and MTCC 2364 (24%) with 30 min infection time, 72 h co-cultivation period and 0.6 OD600. Transformation efficiency was further enhanced to 55 % with 125 μM acetosyringone. MTCC 532 was proven a better strain over the MTCC 2364 in all the tested factors. Putative transgenic hairy roots were confirmed by polymerase chain reaction using rolA specific primers. Biomass of randomly selected 5 hairy root lines was also significantly enhanced. No significant difference of growth was recorded among the lines except line no 4. In the present study, an enhanced system for Agrobacterium rhizogenesmediated hairy root culture was established which offer an effective means to attain improved transformation efficiency and ultimately beneficial for the industrial scale in vitro production of the secondary metabolite.

scholarly journals Agrobacterium rhizogenes Mediated Hairy Root Induction in Parasponia andersonii Planch

2017 ◽  
Vol 16 (4) ◽  
pp. 227-234
Author(s):  
Knyazev Aleksey ◽  
Kuluev Bulat ◽  
Vershinina Zilya ◽  
Yasybaeva Gulnar ◽  
Chemeris Aleksey
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Root Induction ◽  
Hairy Root Induction
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