Applications of immobilized lipases in enzymatic reactors: A review

2022 ◽  
Author(s):  
Daniela Remonatto ◽  
Rodney Helder Miotti Júnior ◽  
Rubens Monti ◽  
Juliana Cristina Bassan ◽  
Ariela Veloso de Paula
Keyword(s):  
Immobilized Lipases

scholarly journals Design of a New Gemini Lipoaminoacid with Immobilized Lipases Based on an Eco-Friendly Biosynthetic Process

Catalysts ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 164
Author(s):  
Patrícia M. Carvalho ◽  
Rita C. Guedes ◽  
Maria R. Bronze ◽  
Célia M. C. Faustino ◽  
Maria H. L. Ribeiro
Keyword(s):  
High Performance ◽  
Transfection Efficiency ◽  
Sol Gel ◽  
Reaction Rates ◽  
Solvent Free ◽  
Bioactive Molecules ◽  
Thermomyces Lanuginosus ◽  
Chemical Production ◽  
Immobilized Lipases ◽  
Free Media

Lipoaminoacids (LAA) are an important group of biosurfactants, formed by a polar hydrophilic part (amino acid) and a hydrophobic tail (lipid). The gemini LAA structures allow the formation of a supramolecular complex with bioactive molecules, like DNA, which provides them with good transfection efficiency. Since lipases are naturally involved in lipid and protein metabolism, they are an alternative to the chemical production of LAA, offering an eco-friendly biosynthetic process option. This work aimed to design the production of novel cystine derived gemini through a bioconversion system using immobilized lipases. Three lipases were used: porcine pancreatic lipase (PPL); lipase from Thermomyces lanuginosus (TLL); and lipase from Rizhomucor miehei (RML). PPL was immobilized in sol-gel lenses. L-cystine dihydrochloride and dodecylamine were used as substrates for the bioreaction. The production of LAA was evaluated by thin layer chromatography (TLC), and colorimetric reaction with eosin. The identification and quantification was carried out by High Performance Liquid Chromatographer-Mass Spectrometry (HPLC-MS/MS). The optimization of media design included co-solvent (methanol, dimethylsulfoxide), biphasic (n-hexane and 2-propanol) or solvent-free media, in order to improve the biocatalytic reaction rates and yields. Moreover, a new medium was tested where dodecylamine was melted and added to the cystine and to the biocatalyst, building a system of mainly undissolved substrates, leading to 5 mg/mL of LAA. Most of the volume turned into foam, which indicated the production of the biosurfactant. For the first time, the gemini derived cystine lipoaminoacid was produced, identified, and quantified in both co-solvent and solvent-free media, with the lipases PPL, RML, and TLL.

Partial purification and characterization of free and immobilized lipases fromMucor miehei

Lipids ◽  
1987 ◽  
Vol 22 (9) ◽  
pp. 680-680
Author(s):  
Birgitte Huge-Jensen ◽  
Donna Rubano Galluzzo ◽  
Robert G. Jensen
Keyword(s):  
Partial Purification ◽  
Purification And Characterization ◽  
Immobilized Lipases

Gas-Phase Enzymatic Esterification on Immobilized Lipases in MCM-41 Molecular Sieves

2002 ◽  
pp. 963-976
Author(s):  
Emerson Luis Pires ◽  
Everson Alves Miranda ◽  
Gustavo Paim Valença
Keyword(s):  
Gas Phase ◽  
Molecular Sieves ◽  
Enzymatic Esterification ◽  
Immobilized Lipases ◽  
Mcm 41

Oxidation kinetics of sardine oil in the presence of commercial immobilized lipases commonly used as biocatalyst

LWT ◽  
2018 ◽  
Vol 96 ◽  
pp. 228-235 ◽  
Author(s):  
Ángela García Solaesa ◽  
María Teresa Sanz ◽  
Rodrigo Melgosa ◽  
Sagrario Beltrán
Keyword(s):  
Oxidation Kinetics ◽  
Sardine Oil ◽  
Immobilized Lipases ◽  
Kinetics Of

scholarly journals Ultrasound affects the selectivity and activity of immobilized lipases applied to fatty acid ethyl ester synthesis

2019 ◽  
Vol 42 ◽  
pp. e46582 ◽  
Author(s):  
José Carlos Quilles Junior ◽  
Ana Lucia Ferrarezi ◽  
Janaina Pires Borges ◽  
Jessika Souza Rossi ◽  
Daniela Alonso Bocchini ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ethyl Ester ◽  
Acid Ethyl Ester ◽  
Fatty Acid Ethyl Ester ◽  
Ethyl Linoleate ◽  
Biodiesel Production ◽  
Novozym 435 ◽  
Thermomyces Lanuginosus ◽  
Ethyl Palmitate ◽  
Immobilized Lipases

Hydrophobic carriers can be used to improve the activity, stability and other properties of enzymes. Physical agents, like ultrasound, may also contribute to improving the dispersion and collision of the reagent molecules, decreasing the reaction time and intensifying the catalytic process. However, its effect on the enzyme activity and reaction selectivity is still not entirely understood. Here, enzyme modulation of immobilized lipases was studied under pulsed ultrasound irradiation in fatty acid ethyl ester (FAEE) synthesis for biodiesel production. Novozym 435® and two commercial lipases from Thermomyces lanuginosus and Rhizomucor miehei, immobilized on Octadecyl-Sepabeads were used as a biocatalyst in the transesterification reaction of vegetable oils and ethanol. The use of ultrasound associated with catalysis by the Novozym 435 increased the production of FAEE by about three times (from 8.9 to 26.4%) using soybean oil and changes were observed in the profile of the products. From the sonicated reaction, ethyl-palmitate production decreased from 23.4 to 11.7%, while the ethyl-linoleate content rose from 47.5 to 59.2%. On the other hand, the T. lanuginosus lipase was less affected by sonication with the overall production of FAEE increasing from 17.2 to 24.1%, with ethyl-palmitate and ethyl-linoleate content changing from 16.2 to 17.5% and 55.0 to 47.8%, respectively. Although the changes in the production yield are not too high, the main idea here was to show that ultrasound modulates the lipase activity as well as its respective selectivity. Thus, ultrasound, is responsible for changing the ethyl ester production, which can be applied to many other biochemical processes to improve or modulate their synthesis yield.

scholarly journals The Immobilized Lipases in Biodiesel Production

10.5772/25246 ◽  
2011 ◽  
Author(s):  
Margarita Stoytcheva ◽  
Gisela Montero ◽  
Lydia Toscano ◽  
Velizar Gochev ◽  
Benjamin Valdez
Keyword(s):  
Biodiesel Production ◽  
Immobilized Lipases
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