Lignocellulosic ethanol production employing immobilized Saccharomyces cerevisiae in packed bed reactor

2016 ◽  
Vol 98 ◽  
pp. 57-63 ◽  
Author(s):  
Abhishek Mishra ◽  
Ajay K. Sharma ◽  
Sumit Sharma ◽  
Rashmi Bagai ◽  
Anshu S. Mathur ◽  
...  
Author(s):  
Lorena Amaya-Delgado ◽  
Guillermo Flores-Cosío ◽  
Dania Sandoval-Nuñez ◽  
Melchor Arellano-Plaza ◽  
Javier Arrizon ◽  
...  

2013 ◽  
Vol 15 (2) ◽  
pp. 94-98 ◽  
Author(s):  
Shengdong Zhu ◽  
Pei Yu ◽  
Mingke Lei ◽  
Yanjie Tong ◽  
Lu Zheng ◽  
...  

Ionic liquid (IL) pretreatment of lignocellulosic materials has provided a new technical tool to improve lignocellulosic ethanol production. To evaluate the influence of the residual IL in the fermentable sugars from enzymatic hydrolysis of IL pretreatment of lignocellulosic materials on the subsequent ethanol fermentation, the toxicity of the IL 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) to Saccharomyces cerevisiae AY93161 was investigated. Firstly, the morphological structure, budding and metabolic activity of Saccharomyces cerevisiae AY93161 at different [BMIM]Cl concentrations were observed under an optical microscope. The results show that its single cell morphology remained unchanged at all [BMIM]Cl concentrations, but its reproduction rate by budding and its metabolic activity decreased with the [BMIM]Cl concentration increasing. The half effective concentration (EC50) and the half inhibition concentration (IC50) of [BMIM]Cl to Saccharomyces cerevisiae AY93161 were then measured using solid and liquid suspension culture and their value were 0.53 and 0.39 g.L-1 respectively. Finally, the influence of [BMIM]Cl on ethanol production was investigated. The results indicate that the [BMIM]Cl inhibited the growth and ethanol production of Saccharomyces cerevisiae AY93161. This toxicity study provides useful basic data for further development in lignocellulosic ethanol production by using IL technology and it also enriches the IL toxicity data.


2020 ◽  
Vol 4 (4) ◽  
pp. 1963-1973 ◽  
Author(s):  
Nulee Jang ◽  
Muhammad Yasin ◽  
Mungyu Lee ◽  
Hyunsoo Kang ◽  
In Seop Chang

A packed-bed reactor (PBR) which has recyclable internal gas and medium exchange functions for carbon monoxide (CO) fermentation was operated using an ethanol producing acetogen, Clostridium autoethanogenum DSM 10061.


2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Cristiane Marques dos Reis ◽  
Edson Luiz Silva

This study evaluated the use of an anaerobic packed-bed reactor for hydrogen production at different hydraulic retention times (HRT) (1–8 h). Two reactors filled with expanded clay and fed with glucose (3136–3875 mg L−1) were operated at different total upflow velocities: 0.30 cm s−1(R030) and 0.60 cm s−1(R060). The effluent pH of the reactors was maintained between 4 and 5 by adding NaHCO3and HCl solutions. It was observed a maximum hydrogen production rate of 0.92 L H2 h−1 L−1in R030 at HRT of 1 h. Furthermore, the highest hydrogen yield of 2.39 mol H2 mol−1glucose was obtained in R060. No clear trend was observed by doubling the upflow velocities at this experiment. High ethanol production was also observed, indicating that the ethanol-pathway prevailed throughout the experiment.


Author(s):  
Thamilmaraiselvi B ◽  
Steffi PF ◽  
Sathammaipriya N ◽  
Sangeetha K

Saccharomyces cerevisiae is a species of yeast. It has been instrumental in winemaking, baking, and brewing since ancient times. The present study was performed to produce lignin degrading enzymes to degrade lignocellulosic substrates and to produce ethanol using Saccharomyces cerevisiae by performing FTIR method. The yeast culture Saccharomyces cerevisiae was isolated and screened for the production of lignolytic enzymes. Then it was pretreated to produce lignocellulosic substrates. Lignocellulosic materials are considered the most abundant renewable resource available for the production of ethanol by FTIR method. The present study concluded the utilization of lignocellulosic biomass for ethanol production in future. Keywords: Saccharomyces cerevisiae; Lignocellulosic; Ethanol; FTIR


2016 ◽  
Vol 10 (1) ◽  
pp. 391-397
Author(s):  
Wangxiang Huang ◽  
Jiancheng Jin ◽  
Liang Feng ◽  
Wenjing Huang ◽  
Ke Wang ◽  
...  

The effects of ionic liquid 1-butyl-3-methylimidazolium chloride (BMIMCl) on the ethanol fermentation process of Saccharomyces cerevisiae AY93161 were investigated by using microcalorimetry. On the basis of microcalorimetric and process data, the thermokinetic parameters of the ethanol fermentation process at different BMIMCl concentrations from 0.001 to 5 gL-1 were calculated. Compared to the control, the BMIMCl caused a decreased value of the maximum specific growth rate µm (from 0.226 to 0.105 h-1), and an increased value of the maximum specific produced heat rate pm (from 2.08 to 7.06 mWlg-1) and the total heat output H for producing 1 g ethanol (from 990 to 1871 Jg-1). The decreased µm and increased pm and H led to lower final yeast concentration (from 3.85 to 2.39 gL-1) and ethanol concentration (from 40.3 to 25.1 gL-1). This gives useful information for improving the lignocellulosic ethanol production process using the ionic liquid technology.


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