Low cost pretreatment of lignocellulosic waste by white rot fungi for ethanol production using Saccharomyces cerevisiae

Saccharomyces cerevisiae is a species of yeast. It has been instrumental in winemaking, baking, and brewing since ancient times. The present study was performed to produce lignin degrading enzymes to degrade lignocellulosic substrates and to produce ethanol using Saccharomyces cerevisiae by performing FTIR method. The yeast culture Saccharomyces cerevisiae was isolated and screened for the production of lignolytic enzymes. Then it was pretreated to produce lignocellulosic substrates. Lignocellulosic materials are considered the most abundant renewable resource available for the production of ethanol by FTIR method. The present study concluded the utilization of lignocellulosic biomass for ethanol production in future. Keywords: Saccharomyces cerevisiae; Lignocellulosic; Ethanol; FTIR

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Research on the changes of physiological characteristics of algal cells in the process of algae dissolving by immobilized white rot fungi

E3S Web of Conferences ◽

10.1051/e3sconf/202016505033 ◽

2020 ◽

Vol 165 ◽

pp. 05033

Author(s):

Guoming Zeng ◽

Jing Luo ◽

Xiaowan Liu ◽

Maolan Zhang ◽

Hengjun Tang

Keyword(s):

Low Cost ◽

White Rot Fungi ◽

Physical Method ◽

White Rot ◽

Ecological Security ◽

Algal Control ◽

The Cost ◽

Water Eutrophication ◽

By Products ◽

Control Water

With the increasing of water pollution, water eutrophication is seriously affecting people’s daily life and production. Therefore, it is particularly important to explore safe and efficient algae control technology. In the current algal bloom treatment methods, the physical method is not complete in algae dissolving, and the cost of algal control is high. The chemical method is easy to produce secondary pollution and toxic by-products, and the safety is not high. However, the biological method has the advantages of low cost, high ecological security and good ecological compatibility. It is considered to be a more promising method to remove algae and biological toxins, and it is also an inevitable trend to control water eutrophication in the future.

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Comparative of Lignocellulosic Ethanol Production by Kluyveromyces marxianus and Saccharomyces cerevisiae

Special Topics in Renewable Energy Systems ◽

10.5772/intechopen.78685 ◽

2018 ◽

Author(s):

Lorena Amaya-Delgado ◽

Guillermo Flores-Cosío ◽

Dania Sandoval-Nuñez ◽

Melchor Arellano-Plaza ◽

Javier Arrizon ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Ethanol Production ◽

Kluyveromyces Marxianus ◽

Lignocellulosic Ethanol

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Bioethanol Production from Pineapple Peel Juice Using Saccharomyces cerevisiae

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.875-877.242 ◽

2014 ◽

Vol 875-877 ◽

pp. 242-245

Author(s):

Jutarut Pornpunyapat ◽

Wilaiwan Chotigeat ◽

Pakamas Chetpattananondh

Keyword(s):

Saccharomyces Cerevisiae ◽

Ethanol Production ◽

Ethanol Concentration ◽

Renewable Resource ◽

Reducing Sugar ◽

Total Sugar ◽

Bioethanol Production ◽

Pineapple Peel ◽

Initial Ph ◽

Production Characteristics

Bioethanol is widely used as renewable resource due to its safe to produce and environmentally friendly. However, knowledge on ethanol production from pineapple peel juice (Pattawia spp) is far from sufficient. In this work, pineapple peel juice (initial pH at 5) was fermented at various yeast contents (1, 3 and 5% by wt.) and fermentation times (3, 5 and 7 days) in order to investigate ethanol production characteristics. Yeast, Sacchromyces cerevisiae was grown on pineapple peel juice. The squeezed juice contained 11% of total sugar and 5% of reducing sugar. The results indicated that the optimum ethanol production was yeast contents of 5% by wt. and fermentation times of 5 days which gave the ethanol production of 9.08g/l. The ethanol at a higher yeast content also had a higher ethanol concentration.

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Investigation of the toxicity of the ionic liquid 1-butyl-3-methylimidazolium chloride to Saccharomyces cerevisiae AY93161 for lignocellulosic ethanol production

Polish Journal of Chemical Technology ◽

10.2478/pjct-2013-0029 ◽

2013 ◽

Vol 15 (2) ◽

pp. 94-98 ◽

Cited By ~ 18

Author(s):

Shengdong Zhu ◽

Pei Yu ◽

Mingke Lei ◽

Yanjie Tong ◽

Lu Zheng ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Ionic Liquid ◽

Ethanol Production ◽

Metabolic Activity ◽

Morphological Structure ◽

Optical Microscope ◽

Reproduction Rate ◽

Lignocellulosic Ethanol ◽

Lignocellulosic Materials ◽

Liquid Suspension

Ionic liquid (IL) pretreatment of lignocellulosic materials has provided a new technical tool to improve lignocellulosic ethanol production. To evaluate the influence of the residual IL in the fermentable sugars from enzymatic hydrolysis of IL pretreatment of lignocellulosic materials on the subsequent ethanol fermentation, the toxicity of the IL 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) to Saccharomyces cerevisiae AY93161 was investigated. Firstly, the morphological structure, budding and metabolic activity of Saccharomyces cerevisiae AY93161 at different [BMIM]Cl concentrations were observed under an optical microscope. The results show that its single cell morphology remained unchanged at all [BMIM]Cl concentrations, but its reproduction rate by budding and its metabolic activity decreased with the [BMIM]Cl concentration increasing. The half effective concentration (EC50) and the half inhibition concentration (IC50) of [BMIM]Cl to Saccharomyces cerevisiae AY93161 were then measured using solid and liquid suspension culture and their value were 0.53 and 0.39 g.L-1 respectively. Finally, the influence of [BMIM]Cl on ethanol production was investigated. The results indicate that the [BMIM]Cl inhibited the growth and ethanol production of Saccharomyces cerevisiae AY93161. This toxicity study provides useful basic data for further development in lignocellulosic ethanol production by using IL technology and it also enriches the IL toxicity data.

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Purification and characterization of heterologous laccase isolated from white-rot fungi Pycnoporus sanguineus in Saccharomyces cerevisiae

Current Opinion in Biotechnology ◽

10.1016/j.copbio.2011.05.255 ◽

2011 ◽

Vol 22 ◽

pp. S84

Author(s):

Funda Ozmen ◽

Esra Varisli ◽

Gunseli Kurt Gur ◽

Ayten Yazgan Karatas ◽

Candan Tamerler

Keyword(s):

Saccharomyces Cerevisiae ◽

White Rot Fungi ◽

White Rot ◽

Purification And Characterization ◽

Pycnoporus Sanguineus

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Effect of initial fermentation medium on bioacetone, biobutanol, and bioethanol (BioABE) production from fermentable sugars of Acacia mangium using Clostridium acetobutylicum YM1

BioResources ◽

10.15376/biores.15.3.6912-6927 ◽

2020 ◽

Vol 15 (3) ◽

pp. 6912-6927

Author(s):

Rafidah Jalil ◽

Mohd Sahaid Kalil ◽

Norliza Abd Rahman ◽

Abdulalati Ibrahim Al-Tabib ◽

Aidil Abdul Hamid ◽

...

Keyword(s):

Clostridium Acetobutylicum ◽

Low Cost ◽

White Rot Fungi ◽

Acacia Mangium ◽

Product Yield ◽

Fermentation Medium ◽

White Rot ◽

Fermentable Sugars ◽

Process Variables ◽

Initial Ph

Bioacetone, biobutanol, and bioethanol (BioABE) production is dependent on the fermentable sugars produced from lignocellulosic biomass and on the composition and initial pH of the medium. Understanding these process variables and their interconnectedness could enhance the BioABE product yield. Acacia mangium is available abundantly and it is a potential feedstock for BioABE production. In this study, BioABE was produced from fermentable sugars of A. mangium using Clostridium acetobutylicum YM1. Alkaline treated A. mangium (70 °C, 3 h, 5.50 %w/v NaOH) was further hydrolyzed via enzymatic hydrolysis using a multi-enzyme of white rot fungi to convert it into fermentable sugars. Approximately 15 g/L of fermentable sugars was produced from A. mangium (100 g/L) and was used for BioABE production in comparison with glucose. Initial findings showed that only 0.94 g/L of BioABE was produced in comparison with glucose (2.86 g/L) at a pH of 6.2. Decreasing the initial pH of the medium to 4.50 increased the BioABE (2.87 g/L), and after the medium was supplemented with tryptone-yeast-acetate (TYA), the BioABE yield increased by more than 100% to 6.84 g/L. This study discovered that BioABE produced from A. mangium was comparable to using commercial glucose, thus offering high potential as a low-cost feedstock.

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Comparison of the Ability of Several White-rot Fungi to Biobleach Acacia Oxygen-delignified Kraft Pulp

Asian Journal of Biotechnology and Bioresource Technology ◽

10.9734/ajb2t/2019/v5i330061 ◽

2019 ◽

pp. 1-10

Author(s):

Sitompul Afrida ◽

Toshihiro Watanabe ◽

Yutaka Tamai

Keyword(s):

Kraft Pulp ◽

Extracellular Enzymes ◽

Low Cost ◽

White Rot Fungi ◽

Acacia Mangium ◽

White Rot ◽

Irpex Lacteus ◽

Lentinus Tigrinus

Previous screening analyses demonstrated that the in vivo biobleaching activities of the white-rot fungi Irpex lacteus KB-1.1 and Lentinus tigrinus LP-7 are higher than those of Phanerochaete chrysosporium and Trametes versicolor. The purpose of the current study was to examine the production of extracellular enzymes of these four white-rot fungi grown on three types of low-cost media containing agricultural and forestry waste, and to evaluate the ability of the produced extracellular enzymes to biobleach Acacia oxygen-delignified kraft pulp (A-OKP). The biobleaching activity of extracellular fractions of I. lacteus, L. tigrinus, T. versicolor, and P. chrysosporium cultures was the most pronounced after 3 days of incubation with Acacia mangium wood powder supplemented with rice bran and 1% glucose (WRBG) with resultant Kappa number reduction of 4.4%, 6.7%, 3.3%, and 3.3%, respectively. Therefore, biobleaching ability of I. lacteus and L. tigrinus have been shown to be higher than of T. versicolor and P. chrysosporium, both in vivo and in vitro.

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