Evaluation of environmental impact on sperm DNA integrity by Sperm Chromatin Dispersion Test and p53 ELISA. Preliminary data (ECOFOODFERTILITY Project)

2015 ◽  
Vol 56 ◽  
pp. 20 ◽  
Author(s):  
Luigi Montano ◽  
Tiziana Notari ◽  
Salvatore Raimondo ◽  
Paolo Bergamo ◽  
Mauro Rossi ◽  
...  
Keyword(s):  
Environmental Impact ◽  
Preliminary Data ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Dispersion Test ◽  
Sperm Chromatin Dispersion Test

scholarly journals In Vitro Studying the Effect of Adding Autologous Platelet Rich Plasma (PRP) to the Human Semen on the Sperm DNA Integrity

2021 ◽  
Vol 10 (2) ◽  
pp. 90-100
Author(s):  
Dhafer Hamdan ◽  
Ali Rahim ◽  
Ula Al-Kawaz
Keyword(s):  
Dna Fragmentation ◽  
Assisted Reproductive Technologies ◽  
Platelet Rich Plasma ◽  
Reproductive Technologies ◽  
Human Reproduction ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Dispersion Test

For conception and the development of healthy embryos, sperm DNA integrity is crucial. According to a growing body of studies, there is a strong correlation between sperm DNA damage and male infertility. Among the new medicines being developed in the medical field, the application of Platelet Rich Plasma (PRP) in human reproduction has yet to be examined. A total of 100 semen samples were used in the current experimental investigation. From November 2020 to June 2021, the research was conducted at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies. Masturbation was used to get an ejaculated semen sample. After semen analysis, the samples were separated into two equal parts, one without autologous PRP and the other with 2% autologous PRP, with the DNA fragmentation assessed using the Sperm Chromatin Dispersion Test. There was highly significant reduction in DNA fragmentation index (p < 0.001). The mean sperm DNA integrity was reduced after adding PRP (33.85±16.73 vs 38.55±16.64), Mean (± SE). PRP has been shown to improve human sperm DNA integrity.

scholarly journals Beneficial effect of antioxidant therapy on sperm DNA integrity is not associated with a similar effect on sperm chromatin integrity

2019 ◽  
Vol 4 ◽  
pp. 31-31 ◽  
Author(s):  
Cécile Le Saint ◽  
Isaac-Jacques Kadoch ◽  
François Bissonnette ◽  
Julie Choi ◽  
Jonathan Zini ◽  
...  
Keyword(s):  
Beneficial Effect ◽  
Antioxidant Therapy ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Chromatin Integrity

Sperm DNA fragmentation: causes and identification

Zygote ◽  
2019 ◽  
Vol 28 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Thais Rose dos Santos Hamilton ◽  
Mayra Elena Ortiz D’Ávila Assumpção
Keyword(s):  
Dna Fragmentation ◽  
Semen Analysis ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Functional Capability ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Fertility Disorders ◽  
Chromatin Integrity

SummarySperm DNA fragmentation is referred to as one of the main causes of male infertility. Failures in the protamination process, apoptosis and action of reactive oxygen species (ROS) are considered the most important causes of DNA fragmentation. Action of ROS or changes in sperm protamination would increase the susceptibility of sperm DNA to fragmentation. Routine semen analysis is unable to estimate sperm chromatin damage. Sperm DNA integrity influences sperm functional capability, therefore tests that measure sperm DNA fragmentation are important to assess fertility disorders. Actually, there is a considerable number of methods for assessing sperm DNA fragmentation and chromatin integrity, sperm chromatin stability assay (SCSA modified), sperm chromatin dispersion (SCD), comet assay, transferase dUTP nick end labelling (TUNEL); and protamine evaluation in sperm chromatin assay, such as toluidine blue, CMA3, protamine expression and evaluation of cysteine radicals. This review aims to describe the main causes of sperm DNA fragmentation and the tests commonly used to evaluate sperm DNA fragmentation.

Evaluation of stallion sperm DNA during cryopreservation using the Toluidine Blue stain and the Sperm Chromatin Dispersion test

2012 ◽  
Vol 32 (8) ◽  
pp. 480 ◽  
Author(s):  
M.I. Carretero ◽  
C.C. Arraztoa ◽  
A. Ferrante ◽  
M. Caldevilla ◽  
R. Santa Cruz ◽  
...  
Keyword(s):  
Toluidine Blue ◽  
Sperm Chromatin ◽  
Sperm Dna ◽  
Blue Stain ◽  
Dispersion Test ◽  
Sperm Chromatin Dispersion Test

Sperm chromatin dispersion test (SCDt) for the assessment of sperm DNA fragmentation in black tiger prawn, Penaeus monodon

Aquaculture ◽  
2018 ◽  
Vol 491 ◽  
pp. 281-288 ◽  
Author(s):  
Tianyi Feng ◽  
Jamie Gosálvez ◽  
Carmen Lopez-Fernandez ◽  
Francisca Arroyo ◽  
Brian Paterson ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Penaeus Monodon ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Sperm Dna ◽  
Black Tiger Prawn ◽  
Tiger Prawn ◽  
Dispersion Test ◽  
Sperm Chromatin Dispersion Test

Assessment of Sperm DNA Fragmentation in Stallion (Equus caballus) and Donkey (Equus asinus) Using the Sperm Chromatin Dispersion Test

2009 ◽  
Vol 44 (5) ◽  
pp. 823-828 ◽  
Author(s):  
EI Cortés-Gutiérrez ◽  
F Crespo ◽  
C Serres-Dalmau ◽  
AL Gutiérrez de las Rozas ◽  
MI Dávila-Rodríguez ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Equus Caballus ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Equus Asinus ◽  
Sperm Dna ◽  
Dispersion Test ◽  
Sperm Chromatin Dispersion Test

170 ASSESSMENT OF FRESH AND FROZEN - THAWED BOTTLENOSE DOLPHIN (TURSIOPS TRUNCATUS) SPERM DNA FRAGMENTATION USING THE SPERM CHROMATIN DISPERSION TEST

2013 ◽  
Vol 25 (1) ◽  
pp. 233
Author(s):  
M. J. Sanchez-Calabuig ◽  
J. de la Fuente ◽  
P. Beltrán-Breña ◽  
E. Martinez-Nevado ◽  
J. F. Perez-Gutierrez ◽  
...  
Keyword(s):  
Bottlenose Dolphin ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Nick Translation ◽  
Neutral Comet Assay ◽  
Sperm Dna ◽  
Dispersion Test ◽  
Dynamic Loss ◽  
Sperm Chromatin Dispersion Test

There has been significant development over the last 20 years to improve genetic management of the captive bottlenose dolphin (Tursiops truncatus) by means of genome resource banking and assisted reproduction. Although standard semen parameters have been analysed in some detail, very little is known about sperm DNA fragmentation (SDF) in this species. The aim of this study was to develop a sperm chromatin dispersion test (SCDt) for the bottlenose dolphin to establish the baseline level of SDF immediately after ejaculation and cryopreservation and to determine the dynamic loss of sperm DNA quality after ex vivo handing and incubation in conditions that mimic the female reproductive tract. Semen from 8 bottlenose dolphins was collected by manual stimulation. Initial validation of the SCDt was conducted by means of in situ nick translation and neutral comet assay using a proven fertile male. To investigate the dynamic loss of sperm chromatin (rate of sDF loss), thawed sperm samples were incubated at 37.9°C for up to 48 h, and aliquots of spermatozoa were assessed after 1, 4, 8, 24, and 48 h. Dolphin sperm nuclei with fragmented DNA exhibited large halos of dispersed DNA fragments, whereas those with nonfragmented DNA displayed small halos of nondispersed DNA. A high correlation (r2 = 0.82; P ≤ 0.01) was found between the respective assessments of the SCDt and the neutral comet assay. All nucleoids resulting in a large halo of dispersed chromatin were intensely positive to in situ nick translation. The level of sDF fragmentation observed immediately after ejaculation in fresh and frozen samples was relatively low (1–5%). After comparing different ejaculates of the same individual, differences were found. Chromatin stability was high during the first 48 h of ejaculation or post-thawing and incubation. Evaluation of the sDF dynamics of fresh and frozen–thawed spermatozoa revealed no significant increase in the baseline level of sDF or in the relative increase of DNA damage after 48 h of incubation. Our data suggest that cryopreservation does not induce a dramatic increase in sperm chromatin damage. Interestingly, sperm samples derived from aged animals resulted in an increased rate of DNA loss, which was observed after 60 min post-incubation.

65 IMPACT OF AIRPORT RADIATION ON BOVINE SPERM DNA INTEGRITY, FERTILIZING ABILITY, AND EMBRYO DEVELOPMENT

2009 ◽  
Vol 21 (1) ◽  
pp. 132
Author(s):  
K. E. M. Hendricks ◽  
D. Evenson ◽  
P. J. Hansen ◽  
M. Kaproth ◽  
L. M. Penfold
Keyword(s):  
Embryo Development ◽  
Blastocyst Stage ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Bovine Sperm ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Fertilizing Ability ◽  
The Mean ◽  
The Impact

Biological samples, including cryopreserved sperm, are routinely shipped using air transportation, in dry shippers that are x-rayed along with routine baggage. Accordingly, it is important to demonstrate that there are no potential risks associated with semen transport. The goal of this study was to investigate the impact of airport radiation used for a) checked luggage and b) carry-on luggage on bovine sperm DNA integrity, fertilizing ability, and embryo development. Frozen domestic bull sperm collected from known fertile bulls (n = 9) and stored in a dry shipper (–196°C) were x-rayed 0, 1, 2, and 3 times as a) checked luggage and b) carry-on luggage. Duplicate straws were thawed and assessed for DNA damage using the sperm chromatin structure assay (SCSA®, SCSA Diagnostics, Brookings, SD) and fertilization and embryo development by in vitro fertilization. The SCSA® parameters are the mean and SD of the DNA fragmentation index (mean DFI and SD DFI). Multiple x-rays did not significantly (P > 0.05) affect sperm chromatin heterogeneity assessed by SCSA® and no differences were observed in the mean, SD, and DFI for any of the sperm treatments. No differences (P > 0.05) were seen in embryo cleavage or blastocyst development rates (expressed as percentage of oocytes becoming blastocysts or percentage of cleaved embryos becoming blastocysts) for sperm x-rayed 0, 1, 2, or 3 times using either checked or carry-on luggage doses. The percentage of oocytes developing to the blastocyst stage was 13.8, 11.5, 12.8, and 9.0% (SEM = 2.3%) for sperm exposed to the checked luggage dose 0, 1, 2, and 3 times. The percentage of oocytes developing to the blastocyst stage was 13.0, 12.8, 14.0, and 13.5% (SEM = 3.5%) for sperm exposed to the carry-on luggage dose 0, 1, 2, and 3 times. As future x-ray machines are planned that deliver greater doses of radiation to scan large quantities of baggage with a single scan, it is important that continued monitoring of shipped sperm is performed. The authors are grateful to Lara Metrione, Brian Delauter, and the TSA staff at Jacksonville Airport for assistance with this study.

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