A combination of direct viable count and fluorescent in situ hybridization for estimating Helicobacter pylori cell viability

2006 ◽  
Vol 157 (4) ◽  
pp. 345-349 ◽  
Author(s):  
Patricia Piqueres ◽  
Yolanda Moreno ◽  
Jose L. Alonso ◽  
Maria A. Ferrús
Keyword(s):  
Helicobacter Pylori ◽  
In Situ Hybridization ◽  
Cell Viability ◽  
Fluorescent In Situ Hybridization ◽  
Viable Count

scholarly journals Molecular detection of Helicobacter pylori in a large Mediterranean river, by direct viable count fluorescent in situ hybridization (DVC-FISH)

2014 ◽  
Vol 12 (4) ◽  
pp. 868-873 ◽  
Author(s):  
Ilias Tirodimos ◽  
Mattheos Bobos ◽  
Evangelos Kazakos ◽  
Anna-Bettina Haidich ◽  
Theodore Dardavessis ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Potable Water ◽  
Molecular Detection ◽  
Viable Count ◽  
Potential Health ◽  
Viable Cells ◽  
H Pylori

Although the precise route and mode of transmission of Helicobacter pylori are still unclear, molecular methods have been applied for the detection of H. pylori in environmental samples. In this study, we used the direct viable count fluorescent in situ hybridization (DVC-FISH) method to detect viable cells of H. pylori in the River Aliakmon, Greece. This is the longest river in Greece, and provides potable water in metropolitan areas. H. pylori showed positive detection for 23 out of 48 water samples (47.9%), while no seasonal variation was found and no correlation was observed between the presence of H. pylori and indicators of fecal contamination. Our findings strengthen the evidence that H. pylori is waterborne while its presence adds to the potential health hazards of the River Aliakmon.

scholarly journals Combination of Direct Viable Count and Fluorescent In Situ Hybridization (DVC-FISH) as a Potential Method for Identifying Viable Vibrio parahaemolyticus in Oysters and Mussels

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1502
Author(s):  
Jorge García-Hernández ◽  
Manuel Hernández ◽  
Yolanda Moreno
Keyword(s):  
In Situ Hybridization ◽  
Vibrio Parahaemolyticus ◽  
Fluorescent In Situ Hybridization ◽  
Potential Method ◽  
Viable Count ◽  
Hybridization Technique ◽  
Fish Technique ◽  
Viable Cells

Vibrio parahaemolyticus is a human food-borne pathogen with the ability to enter the food chain. It is able to acquire a viable, non-cultivable state (VBNC), which is not detected by traditional methods. The combination of the direct viable count method and a fluorescent in situ hybridization technique (DVC-FISH) makes it possible to detect microorganisms that can present VBNC forms in complex samples The optimization of the in vitro DVC-FISH technique for V. parahaemolyticus was carried out. The selected antibiotic was ciprofloxacin at a concentration of 0.75 μg/mL with an incubation time in DVC broth of 5 h. The DVC-FISH technique and the traditional plate culture were applied to detect and quantify the viable cells of the affected pathogen in artificially contaminated food matrices at different temperatures. The results obtained showed that low temperatures produced an important logarithmic decrease of V. parahaemolyticus, while at 22 °C, it proliferated rapidly. The DVC-FISH technique proved to be a useful tool for the detection and quantification of V. parahaemolyticus in the two seafood matrices of oysters and mussels. This is the first study in which this technique has been developed to detect viable cells for this microorganism.

scholarly journals Use of fluorescent in situ hybridization to evidence the presence of Helicobacter pylori in water

2003 ◽  
Vol 37 (9) ◽  
pp. 2251-2256 ◽  
Author(s):  
Y. Moreno ◽  
M.A. Ferrús ◽  
J.L. Alonso ◽  
A. Jiménez ◽  
J. Hernández
Keyword(s):  
Helicobacter Pylori ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization
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